A new transparent multi-unit recording array system fabricated by in-house laboratory technology

Amthor, Franklin R.; Tootle, John S.; Yildirim, Abidin

doi:10.1016/s0165-0270(03)00094-3

Cited by 9 publications

(9 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ganglion cell somas were sometimes visualized using Azure B (Sigma-Aldrich) solution, a few drops of which were added to the superfusate flowing over the retina at the beginning of recording. 30 Under these conditions, retinas can remain stably responsive for 8 or more hours.…”

Section: Methodsmentioning

confidence: 99%

“…Multi-electrode arrays allowed testing the effects of tACS current on neighboring ganglion cells simultaneously. The multi-electrode arrays were similar to those of Amthor et al, 30 except that stainless steel wires were used rather than carbon fibers. Extracellularly recorded signals of spiking ganglion cells were amplified conventionally and digitally stored via a Measurement Computing data acquisition boards (USB-1608 and USB-201, Measurement Computing Corp., Norton, MA) and analyzed offline as described below.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of tACS-Like Electrical Stimulation on On-Center Retinal Ganglion Cells: Part I

Amthor¹,

Strang²

2021

Self Cite

View full text Add to dashboard Cite

Electrical stimulation of the human central nervous system via surface electrodes has been used for both learning enhancement and the amelioration of neurodegenerative or psychiatric disorders. However, data are sparse on how such electrical stimulation affects neural circuits at the cellular level. This study assessed the effects of tACS-like currents at 10 Hz on On-center retinal ganglion cell responsiveness, using the rabbit retina eyecup preparation as a model for central nervous system effects. Methods: We made extracellular recordings of light-evoked spike responses in different classes of On-center retinal ganglion cells before, during and after brief applications of 1 microampere alternating currents using single electrodes and microelectrode arrays. Results: tACS-like currents (tACS) of 1 microampere produced effects on On-center ganglion cell response profiles immediately after initiation or cessation of tACS, without driving phase-locked firing in the absence of light stimuli. tACS affected the initial transient responses to light stimulation for all cells, sustained response components (if any) more strongly for sustained cells, and the center-surround balance more strongly for transient cells. Conclusion: tACS sculpted light-evoked responses that lasted for one or more hours after cessation of current without, itself, directly inducing significant firing changes. Functionally, tACS effects could result in effects on contrast thresholds for both broad classes of cells, but because tACs differentially affects the center-surround balance of transient On-center cells, there may be greater effects on the spatial resolution and gain. The isolated retina appears to be a useful model to understand tACS actions at the neuronal level.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of tACS-Like Electrical Stimulation on On-Center Retinal Ganglion Cells: Part I

Amthor¹,

Strang²

2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Extracellular recordings from single cells were obtained using a single carbon-fiber tipped, copper wire in-glass electrode plated with silver chloride (Amthor et al 2003;Armstrong-James and Millar 1979). The signal was amplified (A-M Systems, Sequim, WA), filtered with a custom-made learning filter to remove 60-Hz noise, thresholded with a custom-made Schmitt trigger, and digitized at a 1-kHz sampling rate using PCI-DAS1002 acquisition board (Measurement Computing, Norton, MA).…”

Section: Data Recording and Analysismentioning

confidence: 99%

Separability of stimulus parameter encoding by on-off directionally selective rabbit retinal ganglion cells

Nowak

Dobbins²,

Gawne³

et al. 2011

Journal of Neurophysiology

View full text Add to dashboard Cite

The ganglion cell output of the retina constitutes a bottleneck in sensory processing in that ganglion cells must encode multiple stimulus parameters in their responses. Here we investigate encoding strategies of On-Off directionally selective retinal ganglion cells (On-Off DS RGCs) in rabbits, a class of cells dedicated to representing motion. The exquisite axial discrimination of these cells to preferred vs. null direction motion is well documented: it is invariant with respect to speed, contrast, spatial configuration, spatial frequency, and motion extent. However, these cells have broad direction tuning curves and their responses also vary as a function of other parameters such as speed and contrast. In this study, we examined whether the variation in responses across multiple stimulus parameters is systematic, that is the same for all cells, and separable, such that the response to a stimulus is a product of the effects of each stimulus parameter alone. We extracellularly recorded single On-Off DS RGCs in a superfused eyecup preparation while stimulating them with moving bars. We found that spike count responses of these cells scaled as independent functions of direction, speed, and luminance. Moreover, the speed and luminance functions were common across the whole sample of cells. Based on these findings, we developed a model that accurately predicted responses of On-Off DS RGCs as products of separable functions of direction, speed, and luminance (r = 0.98; P < 0.0001). Such a multiplicatively separable encoding strategy may simplify the decoding of these cells' outputs by the higher visual centers.

show abstract

“…For eyecup recordings, data were digitized and spike times detected using custom-template software described elsewherẽ Amthor et al, 2003!. For isolated-retina recordings, selected channels of data were digitized with a commercial data-acquisition system~Cyberkinetics!.…”

Section: Preparation and Recordingmentioning

confidence: 99%

Properties of stimulus-dependent synchrony in retinal ganglion cells

et al. 2007

View full text Add to dashboard Cite

Neighboring retinal ganglion cells often spike synchronously, but the possible function and mechanism of this synchrony is unclear. Recently, the strength of the fast correlation between ON-OFF directionally selective cells of the rabbit retina was shown to be stimulus dependent. Here, we extend that study, investigating stimulus-dependent correlation among multiple ganglion-cell classes, using multi-electrode recordings. Our results generalized those for directionally selective cells. All cell pairs exhibiting significant spike synchrony did it for an extended edge but rarely for full-field stimuli. The strength of this synchrony did not depend on the amplitude of the response and correlations could be present even when the cells' receptive fields did not overlap. In addition, correlations tended to be orientation selective in a manner predictable by the relative positions of the receptive fields. Finally, extended edges and full-field stimuli produced significantly greater and smaller correlations than predicted by chance respectively. We propose an amacrine-network model for the enhancement and depression of correlation. Such an apparently purposeful control of correlation adds evidence for retinal synchrony playing a functional role in vision.

show abstract

A new transparent multi-unit recording array system fabricated by in-house laboratory technology

Cited by 9 publications

References 9 publications

Effects of tACS-Like Electrical Stimulation on On-Center Retinal Ganglion Cells: Part I

Effects of tACS-Like Electrical Stimulation on On-Center Retinal Ganglion Cells: Part I

Separability of stimulus parameter encoding by on-off directionally selective rabbit retinal ganglion cells

Properties of stimulus-dependent synchrony in retinal ganglion cells

Contact Info

Product

Resources

About