A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system

Habicher, Judith; Manuel, Remy; Pedroni, Andrea; Ferebee, Charles; Ampatzis, Konstantinos; Boije, Henrik

doi:10.1016/j.gep.2022.119246

Cited by 3 publications

(4 citation statements)

References 41 publications

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“…Cluster MN 1 was differentially enriched with a marker gene of slow MN in zebrafish, pcdh9 (ref. 37 ), while a marker for slow MNs in mammals, sv2a , was not differentially expressed in any of the clusters (Fig. 1f ) 38 .…”

Section: Resultsmentioning

confidence: 92%

Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish

Pallucchi,

Bertuzzi,

Madrid

et al. 2023

Nat Neurosci

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The flexibility of motor actions is ingrained in the diversity of neurons and how they are organized into functional circuit modules, yet our knowledge of the molecular underpinning of motor circuit modularity remains limited. Here we use adult zebrafish to link the molecular diversity of motoneurons (MNs) and the rhythm-generating V2a interneurons (INs) with the modular circuit organization that is responsible for changes in locomotor speed. We show that the molecular diversity of MNs and V2a INs reflects their functional segregation into slow, intermediate or fast subtypes. Furthermore, we reveal shared molecular signatures between V2a INs and MNs of the three speed circuit modules. Overall, by characterizing how the molecular diversity of MNs and V2a INs relates to their function, connectivity and behavior, our study provides important insights not only into the molecular mechanisms for neuronal and circuit diversity for locomotor flexibility but also for charting circuits for motor actions in general.

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Section: Resultsmentioning

confidence: 92%

Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish

Pallucchi,

Bertuzzi,

Madrid

et al. 2023

Nat Neurosci

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“…It indicates that the member of the protocadherin family and cadherin superfamily of transmembrane proteins containing cadherin domains and networks play important roles in the epithelial-telocyte interaction, like in other cells. 72 In conclusion, OPN expression increased in various stages of smoke-associated lung diseases and regulated CSE-induced over-production of cytokines from human bronchial epithelia evidenced by genetically modified cells and animals. CSE-induced lung inflammation and injury reduced in animals with genome or lung conditional OPN knockout.…”

Section: Discussionmentioning

confidence: 88%

“…OPN‐downregulated epithelia activated with CSE mainly suppressed Protocadherin‐9 expression on TCs with or without CSE. It indicates that the member of the protocadherin family and cadherin superfamily of transmembrane proteins containing cadherin domains and networks play important roles in the epithelial‐telocyte interaction, like in other cells 72 …”

Section: Discussionmentioning

confidence: 99%

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Regulatory roles of Osteopontin in lung epithelial inflammation and epithelial‐telocyte interaction

Fu,

Liu,

Shi

et al. 2023

Clinical & Translational Med

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BackgroundLung epithelial cells play important roles in lung inflammation and injury, although mechanisms remain unclear. Osteopontin (OPN) has essential roles in epithelial damage and repair and in lung cancer biological behaviours. Telocyte (TC) is a type of interstitial cell that interacts with epithelial cells to alleviate acute inflammation and lung injury. The present studies aim at exploring potential mechanisms by which OPN regulates the epithelial origin lung inflammation and the interaction of epithelial cells with TCs in acute and chronic lung injury.MethodsThe lung disease specificity of OPN and epithelial inflammation were defined by bioinformatics. We evaluated the regulatory roles of OPN in OPN‐knockdown or over‐expressed bronchial epithelia (HBEs) challenged with cigarette smoke extracts (CSE) or in animals with genome OPN knockout (gKO) or lung conditional OPN knockout (cKO). Acute lung injury and chronic obstructive pulmonary disease (COPD) were induced by smoking or lipopolysaccharide (LPS). Effects of OPN on PI3K subunits and ERK were assessed using the inhibitors. Spatialization and distribution of OPN, OPN‐positive epithelial subtypes, and TCs were defined by spatial transcriptomics. The interaction between HBEs and TCs was assayed by the co‐culture system.ResultsLevels of OPN expression increased in smokers, smokers with COPD, and smokers with COPD and lung cancer, as compared with healthy nonsmokers. LPS and/or CSE induced over‐production of cytokines from HBEs, dependent upon the dysfunction of OPN. The severity of lung inflammation and injury was significantly lower in OPN‐gKO or OPN‐cKO mice. HBEs transferred with OPN enhanced the expression of phosphoinositide 3‐kinase (PI3K)CA/p110α, PIK3CB/p110β, PIK3CD/p110δ, PIK3CG/p110γ, PIK3R1, PIK3R2 or PIK3R3. Spatial locations of OPN and OPN‐positive epithelial subtypes showed the tight contact of airway epithelia and TCs. Epithelial OPN regulated the epithelial communication with TCs, and the down‐regulation of OPN induced more alterations in transcriptomic profiles than the up‐regulation.ConclusionOur data evidenced that OPN regulated lung epithelial inflammation, injury, and cell communication between epithelium and TCs in acute and chronic lung injury. The conditional control of lung epithelial OPN may be an alternative for preventing and treating epithelial‐origin lung inflammation and injury.

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Perturbed development of calb2b expressing dI6 interneurons and motor neurons underlies locomotor defects observed in calretinin knock-down zebrafish larvae

Iglesias Gonzalez,

Koning,

Tuz-Sasik

et al. 2024

Developmental Biology

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A new transgenic reporter line reveals expression of protocadherin 9 at a cellular level within the zebrafish central nervous system

Cited by 3 publications

References 41 publications

Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish

Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish

Regulatory roles of Osteopontin in lung epithelial inflammation and epithelial‐telocyte interaction

Perturbed development of calb2b expressing dI6 interneurons and motor neurons underlies locomotor defects observed in calretinin knock-down zebrafish larvae

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