A new Toxoplasma gondii chimeric antigen containing fragments of SAG2, GRA1, and ROP1 proteins—impact of immunodominant sequences size on its diagnostic usefulness

Ferra, Bartłomiej; Holec-Gąsior, Lucyna; Kur, Józef

doi:10.1007/s00436-015-4552-6

Cited by 35 publications

(37 citation statements)

References 29 publications

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“…Recent studies revealed that choosing recombinant chimeric multiepitope antigens (54,55) was instrumental in improving the performance of commercialized enzyme immunoassay kits in detecting T. gondii-specific antibodies, using either soluble antigens from crude extracts or recombinant antigens (56)(57)(58). The selection of the reagent that allows for both IgG and IgM detection should take into account the assay's performance under both routine conditions and specified clinical settings (such as high sensitivity for IgM in pregnancy and for IgG in immunosuppressed patients).…”

Section: Discussionmentioning

confidence: 99%

Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis

et al. 2016

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h Toxoplasmosis, a benign infection, is asymptomatic or paucisymptomatic in over 80% of cases, except in immunocompetent patients suffering from ocular toxoplasmosis or in immunocompromised patients with opportunistic or congenital toxoplasmosis. Diagnosis is based mainly on serology testing. Thus, we compared the performance of the nine most commonly used commercial automated or semiautomated immunoassays for IgG and IgM Toxoplasma gondii antibody detection, that is, the Advia Centaur, Architect, AxSYM, Elecsys, Enzygnost, Liaison, Platelia, VIDAS, and VIDIA assays. The assays were conducted on four panels of serum samples derived during routine testing from patients with an interfering disease and who exhibited a low IgG antibody level in one of two clinical settings, namely, acute or chronic toxoplasmosis. As a result, IgG sensitivities ranged from 97.1% to 100%, and IgG specificities ranged from 99.5% to 100%. For IgG quantification, strong differences in IgG titers (expressed in IU/ml) were noted depending on the assay used. IgM sensitivities ranged from 65% to 97.9%, and IgM specificities ranged from 92.6% to 100%. For defining the best serological strategies to be implemented, it appears crucial to compare the diagnostic performance of the different tests with respect to their specificity and sensitivity in detecting the presence of IgG and IgM antibodies.

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Section: Discussionmentioning

confidence: 99%

Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis

et al. 2016

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“…Some studies suggested ROP1 as one of the potent stimulators of mouse cell‐mediated and humoral immunities (Ferra et al. ). It was revealed by the work of Yuan that ROP16 could be used to produce anti‐ T. gondii DNA vaccines and that CTLs specific to T. gondii were enhanced by a plasmid containing a ROP18 gene (Yuan et al.…”

mentioning

confidence: 99%

“…In addition, genes that encode specific ROPs of T. gondii have been regarded as ideal materials for producing anti-T. gondii DNA vaccines. Some studies suggested ROP1 as one of the potent stimulators of mouse cellmediated and humoral immunities (Ferra et al 2015). It was revealed by the work of Yuan that ROP16 could be used to produce anti-T. gondii DNA vaccines and that CTLs specific to T. gondii were enhanced by a plasmid containing a ROP18 gene (Yuan et al 2011a,b).…”

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confidence: 99%

The Molecular Characterization and Immunity Identification of Rhoptry Protein 22 of Toxoplasma gondii as a DNA Vaccine Candidate Against Toxoplasmosis

Zhang

Xie

et al. 2018

J Eukaryotic Microbiology

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Toxoplasma gondii (T. gondii) rhoptry proteins (TgROPs) have been considered main targets and indicator molecules for immune diagnosis and prophylaxis since they initially present during the process of invasion. In this study, the effect of intramuscularly injecting the genetic vaccine pVAX-ROP22 was evaluated, made by inserting the TgROP22 sequence into the eukaryotic expression vector of pVAX I, into BALB/c mice. The levels of IgG, IgG1, and IgG2a in pVAX-ROP22 vaccinated animals were integrally increased. It was uncovered by cytokine profile analyses that the levels of IFN-γ and IL-2 were significantly increased, while no significant changes were detected in IL-4 and IL-10 levels. In addition, we found that immunization with pVAX-ROP22 significantly prolonged the survival time (13.80 ± 1.75 d) of mice after challenge infection with the virulent T. gondii RH strain, in comparison with those of control animals (died within 10 d). Moreover, the number of brain cysts (1,406 ± 277) in the animals subjected to pVAX-TgROP22 vaccination decreased remarkably (P < 0.05) compared with the blank control mice (2,333 ± 473), and the size of brain cysts in pVAX-TgROP22 group was significantly smaller than the groups of blank, PBS and pVAXI. These results suggested that TgROP22 as DNA vaccine could trigger strong humoral and cellular responses and induce partial protection against toxoplasmosis.

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“…Ferrandiz et al reported a sensitivity of the protein of 78.2% in chronic infection against 34% in acute infection [48]. However, recent serological studies showed that GRA1 can be used to detect specific IgG in sera of both acute and chronic phase of disease with a similar sensitivities [33,45,51]. In our study, GRA1 was identified in ST-32 kDa and ST-33 kDa bands that showed a sensitivity of 93.3% and 71.3%, respectively, and a specificity of 92.0% and 93.0%, respectively; and in MT-30 kDa and MT-32 kDa bands that showed a sensitivity of 76.2% and 62.7%, respectively and a specificity of 99.0% and 97.9% respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The protein is secreted into the parasitophore vacuole and plays an important role in host cell invasion [47][48][49]. GRA1 is also associated with strong stimulation of the host immune system [47,50,51]. The overall sensitivity of ELISA using the recombinant form of the protein in the detection of anti-Toxoplasma IgG varies from 60% to 98% [33,47,48,52].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Soluble and Membrane-Bound Proteins of Toxoplasma gondii as Diagnostic Markers of Infection

Khammari¹,

Lakhal²,

Westermann³

et al. 2015

J Bacteriol Parasitol

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In the present study, we applied the combination of one-dimensional gel electrophoresis, immunoblot and nanoliquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) to identify potential immunogenic proteins of Toxoplasma gondii tachyzoites that can be used for the development of reliable assays in the serodiagnosis of acquired toxoplasmosis in immunocompetent subjects. For this purpose, we developed an immunoblot using soluble and membrane extracts of GT1 Toxoplasma gondii tachyzoites and tested 194 positive and 100 negative sera obtained from pregnant women.Five bands of soluble antigens (98 kDa, 36 kDa, 33 kDa, 32 kDa and 21 kDa) and 4 bands of membrane antigens (41 kDa, 35 kDa, 32 kDa and 30 kDa) were selected as the most valuable in terms of sensitivity and specificity. Among these bands, only 2 bands of soluble antigen (33 kDa and 32 kDa) and 2 bands of membrane antigen (32 kDa and 30 kDa) showed a specificity ≥ 90%.After mass spectrometry and bioinformatics analysis, 7 proteins were identified as potential markers for serodiagnosis of toxoplasmosis. These proteins are: SRS34A, GRA7, GRA1, DG32, MIC5, ROP5 and Toxofilin. These proteins showed a 86% to 100% homology with proteins of both VEG and ME49 strains of T. gondii and a 58% to 87% homology with Hammondia hammondi; and can be considered as attractive candidates for the development of an immunochromatography test that can be used for the rapid diagnosis of toxoplasmosis and as a confirmatory test when routine techniques give equivocal results.

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A new Toxoplasma gondii chimeric antigen containing fragments of SAG2, GRA1, and ROP1 proteins—impact of immunodominant sequences size on its diagnostic usefulness

Cited by 35 publications

References 29 publications

Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis

Help in the Choice of Automated or Semiautomated Immunoassays for Serological Diagnosis of Toxoplasmosis: Evaluation of Nine Immunoassays by the French National Reference Center for Toxoplasmosis

The Molecular Characterization and Immunity Identification of Rhoptry Protein 22 of Toxoplasma gondii as a DNA Vaccine Candidate Against Toxoplasmosis

Characterization of Soluble and Membrane-Bound Proteins of Toxoplasma gondii as Diagnostic Markers of Infection

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