A New Tomato-Infecting Tospovirus from Iran

Hassani-Mehraban, A.; Saaijer, Janneke; Peters, D.; Goldbach, Rob; Kormelink, Richard

doi:10.1094/phyto-95-0852

Cited by 64 publications

(51 citation statements)

References 30 publications

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“…To avoid formation of defective interfering (DI) isolates due to serial mechanical passages, new virus inocula were prepared from liquid nitrogen stocks every 4-5 passages [18]. TYRV and the soybean isolate were inoculated on a large range of plant species including those tested for TYRV [15] and different Nicotiana species and N. tabacum cultivars. The plants were monitored for the expression of symptoms during several weeks while being kept at 22-25 • C 12 h (light/dark) under greenhouse conditions.…”

Section: Virus Isolates and Host Range Studymentioning

confidence: 99%

“…The plants were monitored for the expression of symptoms during several weeks while being kept at 22-25 • C 12 h (light/dark) under greenhouse conditions. To confirm systemic infections in infected plants, both symptomatic Differentiation of TYRV isolates 87 and asymptomatic leaf samples, next to uninfected leaf material as negative control, were tested by DAS-ELISA using polyclonal antiserum directed to the N protein of TYRV [7,15].…”

Section: Virus Isolates and Host Range Studymentioning

confidence: 99%

“…However, serological differentiation soon appeared insufficient as a taxonomic criterion, and nowadays the N protein sequence in combination with biological characters such as thrips vector species and host range represent the main classification criteria for the establishment of a new tospovirus species, with the N protein sequence identity threshold set at 90% [5,11]. Based on these criteria, 16 tospovirus species have been recognized with the largest diversity being observed within the Asian continent [15]. With its worldwide distribution and wide host range tomato spotted wilt virus (TSWV) is the most prominent member of all tospoviruses.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, an isolate of a new tentative tospovirus species, named Tomato yellow ring virus (TYRV), was collected from tomato in Iran, and this virus was most closely related (by N protein sequence identity) to iris yellow spot virus (IYSV) [3,15]. In a limited field survey, mainly in Teheran province, Iran, four additional tospovirus isolates were collected from chrysanthemum, gazania, potato, and soybean during [2000][2001][2002].…”

Section: Introductionmentioning

confidence: 99%

“…In a limited field survey, mainly in Teheran province, Iran, four additional tospovirus isolates were collected from chrysanthemum, gazania, potato, and soybean during [2000][2001][2002]. Upon further analysis, the first two isolates were found to represent TYRV isolates [15], whereas preliminary RT-PCR analyses of their N gene suggested that the isolates from soybean and potato represented a different tospovirus.…”

Section: Introductionmentioning

confidence: 99%

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Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept

et al. 2006

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Summary. Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus),one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared. Their genomic S RNA segments differed in size by 55 nucleotides.Comparison of the S RNA intergenic regions revealed the absence of a stretch of 115 nucleotides within the S RNA segment of TYRV-s and, conversely, of 56 nts in that of TYRV-t, apparently a stable genetic difference as it was also found in another isolate of TYRV-s collected from potato. Sequence comparison of the N protein ORFs revealed an identity of 92% between the N proteins of both strains, and the observed strong cross-reaction of TYRV-s in DAS-ELISA with a polyclonal antiserum directed against the TYRV-t N protein confirmed this high identity. Host range analysis revealed several differences, e.g. TYRV-s, but not TYRV-t, being able to systemically infect Nicotiana species, and TYRV-s being localised in tomato. The observed molecular and biological differences of both viruses call into question the currently used criteria for Tospovirus species demarcation.

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Section: Virus Isolates and Host Range Studymentioning

confidence: 99%

Section: Virus Isolates and Host Range Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept

et al. 2006

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Molecular Techniques for Detection of Microbial Pathogens

Narayanasamy

Molecular Biology in Plant Pathogenesis and Disease Management

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Detection, identification and differentiation of microbial plant pathogens -oomycetes, fungi, bacteria, phytoplasmas, viruses and viroids -infecting various crops constitute the basic step for the development of effective crop disease management systems. The conventional cultural methods involving isolation and studying the morphological characteristics using microscope are labor intensive and cumbersome, yielding sometimes, inconclusive results. The molecular techniques, on the other hand, are able to provide precise, reliable and reproducible results rapidly, facilitating early disease management decisions. Biochemical, immunological and nucleic acid-based assays have been preferred, because of the distinct advantages over the conventional methods. The molecular techniques have been very useful in the identification of obligate pathogens causing diseases such as downy mildews, powdery mildews and rusts and also fungi that grow very slowly in the culture media, taking several weeks to produce spore forms that can be used for identification. The availability of antisera, primers and commercial kits has led to widespread application of the molecular techniques for on-site detection during field surveys for assessing the distribution of existing pathogens, occurrence of new/introduced pathogens or strains and for preventing introduction of new pathogens through seeds or planting materials. Furthermore, molecular techniques have been demonstrated to be useful in breeding programs to identify sources of resistance to disease(s). Several protocols for molecular techniques, useful for researchers and students are presented in the Appendix.

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