A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus

Wang, Weihong; Park, Kyu-Hyung; Lee, Jusung; Oh, Eunseok; Park, Chanyoon; Kang, Eunmo; Lee, Juni; Kang, Heonjoong

doi:10.3390/molecules25194383

Cited by 11 publications

(5 citation statements)

References 17 publications

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“…More recent work includes the ribosomal production of micrococcin P1 and micrococcin P2 antibiotics through prepeptide gene replacement [37]. Most recently, the antibiotic thiopeptide micrococcin P3 was isolated from a marine-derived strain of Bacillus stratosphericus [38]. In this study, we established the following MS-MS fragmentation pattern for micrococcin P1 (1) from the maXis 4G measurements (Table 3) that unequivocally matched with that reported by Walsh et al, in 2010 [37].…”

Section: Isolation Of Micrococcin P1 (1) and Micrococcin P2 (2)supporting

confidence: 81%

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

2022

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Epibiotic bacteria associated with the filamentous marine cyanobacterium Moorea producens were explored as a novel source of antibiotics and to establish whether they can produce cyclodepsipeptides on their own. Here, we report the isolation of micrococcin P1 (1) (C48H49N13O9S6; obs. m/z 1144.21930/572.60381) and micrococcin P2 (2) (C48H47N13O9S6; obs. m/z 1142.20446/571.60370) from a strain of Bacillus marisflavi isolated from M. producens’ filaments. Interestingly, most bacteria isolated from M. producens’ filaments were found to be human pathogens. Stalked diatoms on the filaments suggested a possible terrestrial origin of some epibionts. CuSO4.5H2O assisted differential genomic DNA isolation and phylogenetic analysis showed that a Kenyan strain of M. producens differed from L. majuscula strain CCAP 1446/4 and L. majuscula clones. Organic extracts of the epibiotic bacteria Pseudoalteromonas carrageenovora and Ochrobactrum anthropi did not produce cyclodepsipeptides. Further characterization of 24 Firmicutes strains from M. producens identified extracts of B. marisflavi as most active. Our results showed that the genetic basis for synthesizing micrococcin P1 (1), discovered in Bacillus cereus ATCC 14579, is species/strain-dependent and this reinforces the need for molecular identification of M. producens species worldwide and their epibionts. These findings indicate that M. producens-associated bacteria are an overlooked source of antimicrobial compounds.

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Section: Isolation Of Micrococcin P1 (1) and Micrococcin P2 (2)supporting

confidence: 81%

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

2022

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“…They also have applications in medicine, as siderophoreantibiotic conjugates are effective against multi-drugresistant pathogens, and can be used as imaging agents, and biosensors for metal ions and pathogens (Fan and Fang 2021;Ghosh et al 2020). Thiopeptides are also useful in medicine where they can be applied as antibiotics (Wang et al 2020). AMPs with haemolytic or antibacterial properties were detected in all samples, except for the one collected at level 6.…”

Section: Discussionmentioning

confidence: 98%

A first insight into the Polish Bochnia Salt Mine metagenome

Lach

Królikowska

Baranowska

et al. 2023

Environ Sci Pollut Res

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The Bochnia Salt Mine is one of the oldest mines in Europe. It was established in the thirteenth century, and actively operated until 1990. The mine has been placed on the UNESCO World Heritage List. Previous research describing Polish salt mines has been focused on bioaerosol characteristics and the identification of microorganisms potentially important for human health. The use of Polish salt mines as inhalation chambers for patients of health resorts has also been investigated. Nevertheless, the biodiversity of salt mines associated with biotechnological potential has not been well characterized. The present study paper examines the biodiversity of microorganisms in the Bochnia Salt Mine based on 16S rRNA gene and shotgun sequencing. Biodiversity studies revealed a significantly higher relative abundance of Chlamydiae at the first level of the mine (3.5%) compared to the other levels (< 0.1%). Patescibacteria microorganisms constituted a high percentage (21.6%) in the sample from site RA6. Shotgun sequencing identified 16 unique metagenome-assembled genomes (MAGs). Although one was identified as Halobacterium bonnevillei, the others have not yet been assigned to any species; it is possible that these species may be undescribed. Preliminary analyses of the biotechnological and pharmaceutical potential of microorganisms inhabiting the mine were also performed, and the biosynthetic gene cluster (BGC) profiles and antimicrobial peptide (AMP) coding genes in individual samples were characterized. Hundreds of BGCs and dozens of AMP coding genes were identified in metagenomes. Our findings indicate that Polish salt mines are promising sites for further research aimed at identifying microorganisms that are producers of potentially important substances with biotechnological and pharmaceutical applications.

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“…In contrast, the antibacterial activities of 73 and 74 against V. parahaemolyticus, Photobacterium damselae, Shewanella algae, Bacillus amyloliquefaciens sspplantarum, and Pseudomonas stutzeri (0.5-8 µg/mL) were lower than those of the positive controls. Both compounds did not show cytotoxicity up to a concentration of 10 µM in the MTT assay [69]. Compounds 75-78 displayed antibacterial activity against P. aeruginosa PA-01 and Acinetobacter baumannii ATCC19606 with MIC values 58-64 µg/mL, while the positive control ofloxacin showed MIC values of 1-16 µg/mL [70].…”

Section: Antimicrobial Activitiesmentioning

confidence: 95%

“…Figure 9 lists the other compounds produced by marine-derived Bacillus species. A novel thiopeptide named micrococcin P3 (73) and a known compound named micrococcin P1 (74) were isolated from the fermentation broth of B. stratosphericus [69]. Five new bacillamidins A (75), B (76), C (77), D (78) and E (79), along with two known synthetic analogs, bacillamidins F (80) and G (81), were isolated from the marine-derived B. pumilus strain RJA1515.…”

Section: Other Compoundsmentioning

confidence: 99%

Secondary Metabolites from Marine-Derived Bacillus: A Comprehensive Review of Origins, Structures, and Bioactivities

et al. 2022

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The marine is a highly complex ecosystem including various microorganisms. Bacillus species is a predominant microbialflora widely distributed in marine ecosystems. This review aims to provide a systematic summary of the newly reported metabolites produced by marine-derived Bacillus species over recent years covering the literature from 2014 to 2021. It describes the structural diversity and biological activities of the reported compounds. Herein, a total of 87 newly reported metabolites are included in this article, among which 49 compounds originated from marine sediments, indicating that marine sediments are majority sources of productive strains of Bacillus species Therefore, marine-derived Bacillus species are a potentially promising source for the discovery of new metabolites.

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A New Thiopeptide Antibiotic, Micrococcin P3, from a Marine-Derived Strain of the Bacterium Bacillus stratosphericus

Cited by 11 publications

References 17 publications

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

Micrococcin P1 and P2 from Epibiotic Bacteria Associated with Isolates of Moorea producens from Kenya

A first insight into the Polish Bochnia Salt Mine metagenome

Secondary Metabolites from Marine-Derived Bacillus: A Comprehensive Review of Origins, Structures, and Bioactivities

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