A New Technique for the Recovery of Infective Strongyle Larvae from Soil and Pasture

Abstract

A new technique for the recovery of Trichostrongyle larvae from soil and pasture samples is described.The technique is based on the difference in density of larvae and debris under the influence of a stream of water flowing upwards at a gradually decreasing flow rate.The results from trials with known numbers of larvae gave an average recovery of 74% from pasture, and of 78% from soil.

Evaluation of modified Baermann apparatus for the recovery of infective trichostrongylid larvae from herbage samples

“…DISCUSSION Pasture larval counts have been used to great advantage by several investigators for estimating levels of contamination of pasture with the L3 of many species of nematode parasites of grazing animals (CROFTON, 1954;MICHEL et al, 1970;GIIISON & EVERETT, 1973;GALLIE & NUNNS, 1976). Some of the techniques available are elaborate but efficient (DURIE, 1959;DONALD, 1967;SMEAL & HENDRY, 1972;JORGENSEN, 1975); others, e.g., the standard Baermann technique and the methods of PARFITT(1955) and LANCASTER (1970) are simpler but less efficient.…”

A Technique for the Recovery of Strongyloid Infective Larvae from Pasture

“…The method used for washing herbage and for collection of larvae in the sediment is similar to procedures, described by previous workers (Durie, 1959;Donald, 1907;Heath and Major, 1968). The pasture collected from each traverse is placed in plastic containers of 45 1 capacity and soaked overnight in 91 of water to which has been added 1 ml of a non-ionic detergent ("Lissapol NDB", Imperial Chemical Industries Ltd., or "Nisant E44", Polymer Corporation).…”

The Recovery of Infective Nematode Larvae from Herbage Samples

“…Furthermore, this recovery rate has been determined by adding larvae at various stages during the process. For example, Parfitt (1955) and Heath and Major (1968) added larvae to grass, whilst Durie (1959) and Donald (1967) added larvae to the deposit obtained from washed herbage on the supposition that if larvae were added to grass it did not simulate the relationship of natural populations on herbage.…”