A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process

Huang, Lien‐Hung; Rau, Cheng-Shyuan; Zeng, Wen-Hong; Lu, Tsu-Hsiang; Wu, Yi-Chan; Chiu, Yi-Hong; Wu, Kaiming; Tsai, Chia‐Wen; Lin, Chia-Wei; Wu, Chi; Chung, Yi‐Chang; Hsieh, Ching-Hua

doi:10.1016/j.biopha.2022.113481

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…Surviving curves were determined upon 24 h of GNDs(S) treatments on glioma (C6 and U87-MG) and fibroblast (3T3 and CG1639) cell lines to assess the cytotoxicity of the as-prepared GNDs(S) nanoparticles (Figure ). Above all, we employed optical microscopy to visualize and locate GNDs(S) inside the cells that express gelatinolytic activity. ,− Figure A shows a large number of GNDs(S) locations inside all of the cell lines. The black spots in the figures are the GNDs aggregates surrounding the perinuclear region.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanodandelions as Nanozymes and Reactive Oxygen Species Scavengers in Tumor Microenvironment Components

Lee,

Chuang,

Tsai

et al. 2023

ACS Appl. Nano Mater.

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Gold nanomaterials have attracted increasing attention in biomedical applications due to their biocompatibility and intrinsic optical and physicochemical properties. Oxidative stress, which is mainly contributed by reactive oxygen species (ROS), has been crucial in various cancers and diseases. Here, we report a gold nanodandelion (GND)-based ROS-scavenging system using the antioxidant properties of GNDs. GNDs scavenge superoxide anions and hydroxyl radicals in situ and induce oxidative etching with hydrogen peroxide (H 2 O 2 ). The size and surface passivation of gold nanomaterials regulated the efficiency of ROS scavenging. The differential effect of GNDs to scavenge intracellular ROS was studied through cell viability, nanoparticle uptake, ROS production, and antioxidant enzymes. Results indicated that GNDs in normal fibroblasts and macrophages display minimal toxicity and provide protection from H 2 O 2 -induced oxidative stress. For the first time, GNDs exhibit outstanding nitric oxide (NO • ) generation performance in M1 macrophage. Interestingly, the presence of GNDs in glioma cells attenuated cell proliferation, migration, and invasion behaviors. GNDs are expected to be a promising antioxidant in cancer treatment and tumor microenvironment modulation.

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Section: Resultsmentioning

confidence: 99%

Gold Nanodandelions as Nanozymes and Reactive Oxygen Species Scavengers in Tumor Microenvironment Components

Lee,

Chuang,

Tsai

et al. 2023

ACS Appl. Nano Mater.

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“…It is possible that centrifugation at higher speeds and/or longer times would produce better yields, but this would require more expensive equipment and longer wait times in the clinic. It seems likely that methods other than single-spin centrifugation will be needed to enhance successful separation of platelets from other blood components [46].…”

Section: Discussionmentioning

confidence: 99%

Optimizing Platelet-Rich Plasma: Spin Time and Sample Source

Harrison,

Bowler,

Cheng

et al. 2023

Bioengineering

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The buff-colored layer separating the plasma from red blood cells (RBCs) in centrifuged blood was named the “buffy coat” in the late 19th century. The division of platelets (PLTs) and leukocytes (WBCs) between the buffy coat, plasma, and RBC layers in centrifuged blood has not been described before. In this study, we centrifuged 8.5 mL anticoagulated blood samples at 1000× g for 1, 2, 3, 5, 10, and 20 min. We then divided each sample into ten layers and analyzed each layer for cellular composition and mean platelet volume (MPV). Our results show that even after 20 min of centrifugation, about 15% of platelets remain in the plasma layers and 65% in the RBC layers. We found that the platelet count achieved from aspiration of 1 mL volume was optimal, with aspiration beginning 1/2 mL below the buffy coat and extending 1/2 mL above the buffy coat rather than beginning at the buffy coat itself and aspirating only plasma. Using this method of aspiration, we found that the total platelet count means reached a maximum in the 1 mL around the buffy coat after only 5 min of centrifugation.

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“…Autologous PRP products are complex and naturally variable in their composition and the need for thorough quality controls in view of clinical applications makes it crucial to demonstrate consistency in the manufacturing process [ 4 , 15 , 51 , 52 ]. General protocols for PRP preparation consist of blood collection and a first centrifugation step to separate the red blood cells (RBC), which is then followed by a subsequent second centrifugation step to concentrate the platelets and other components and to provide activation of the biological product (i.e., mechanical action of injection, Figure S2 ) [ 61 , 62 , 63 , 64 , 65 , 66 ].…”

Section: Importance Of Manufacturing Process Standardization For Ther...mentioning

confidence: 99%

Current Status of PRP Manufacturing Requirements & European Regulatory Frameworks: Practical Tools for the Appropriate Implementation of PRP Therapies in Musculoskeletal Regenerative Medicine

Sebbagh

Cannone²,

Gremion

et al. 2023

Bioengineering

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Providing accurate and up-to-date practical tools enabling oversight of platelet-rich plasma (PRP) legislation and of the appropriate standards to be implemented for its manufacture and use in Europe is a demanding task. This is due to rapid medico-technological advancements, slowness and disparity in legislation updates and enforcement between member states, and many reported gray-zone practices, notably for autologous PRP use. The levels of risk associated with blood manipulation processes generally dictate the manufacturing requirements for PRP preparations, which have gradually shifted toward good manufacturing practices (GMP) for standardization and overall quality enhancement. This work firstly outlines Western European and Swiss legislation for PRP products/preparations, providing key simplified information and recommendations for medical doctors seeking to implement this biological-based therapy for safe use in hospital settings, clinics, or private offices. This work secondly shows the importance of PRP-based product manufacturing standardization, which subsequently enables sound clinical evaluation of therapeutic interventions. Although the applicable legal bases provide guidelines for GMP manufacturing infrastructure and basic process design, paramount importance is set on the definition of workflows, technical specifications, and key parameters for PRP preparation and delivery. Overall, the development of simple and robust technologies and processes for PRP preparation is critical for guaranteeing the high therapeutic quality of the intervention, in collaboration with qualified GMP manufacturing platforms. Importantly, this work aims to serve as a practical tool for clinicians based in Western Europe who are willing to appropriately (i.e., administratively and technically) implement autologous PRP treatments in musculoskeletal regenerative medicine workflows, to ensure they make informed and optimal regulatory or process-based decisions.

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A new technique for separating platelet-rich plasma by a copolymer device － without a centrifugation process

Cited by 6 publications

References 35 publications

Gold Nanodandelions as Nanozymes and Reactive Oxygen Species Scavengers in Tumor Microenvironment Components

Gold Nanodandelions as Nanozymes and Reactive Oxygen Species Scavengers in Tumor Microenvironment Components

Optimizing Platelet-Rich Plasma: Spin Time and Sample Source

Current Status of PRP Manufacturing Requirements & European Regulatory Frameworks: Practical Tools for the Appropriate Implementation of PRP Therapies in Musculoskeletal Regenerative Medicine

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