A new technique for promoting cyclic utilization of cyclodextrins in biotransformation

Abstract

Cyclodextrins (CDs) can improve the productivity of steroid biotransformation by enhancing substrate solubility. CDs can be recycled by grafting them with appropriate carriers. Loofah fiber is an excellent grafting material for CDs, and can be applied to the biotransformation and recycling of β-cyclodextrin (β-CD). In this work, a technique for recycling β-CD in cortisone acetate (CA) biotransformation by Arthrobacter simplex CPCC 140451 was studied. Loofah fiber-grafted β-CD (LF-β-CD) was prepared using epichlorohydrin, which is a cross-linking agent. The grafting yield of β-CD was 74.8 mg g dried fibers. LF-β-CD could increase the solubility of CA and enhance biotransformation. The initial conversion rate of CA was 1.5-fold higher than that of the blank group. LF-β-CD was also used in biocatalytic reactions for eight cycles, and it maintained the conversion ratio of CA at approximately 90%. Given the above positive results, LF-β-CD can be utilized in biotechnological recycling applications. This method can also be applied to CD derivatives and hydrophobic compounds.

Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

“…This is consistent with previous observations [ 21 , 22 ]. However, enzyme activity was inhibited by exposure to 1 mM CuCl 2 , FeCl 3 , ZnCl 2 , CdCl 2 , NiCl 2 , or CoCl 2 , and even more so at concentrations of 5 mM, consistent with the results from another report [ 23 ], although these data conflict with the results reported by Birol et al [ 24 ]. No apparent effects on dextranase activity were observed after exposure to BaCl 2 , NH 4 Cl, CaCl 2 , KCl, or LiCl.…”

“…However, to the best of our knowledge, this is the first report of the purification and characterization of dextranase from Catenovulum. Purification of crude dextranase by ammonium sulfate fractionation and Sepharose 6B chromatography, which resulted in a 6.69-fold increase in specific activity and an 11.27% recovery, was previously reported [ 23 ]. This system of the aforementioned procedure may be used to produce homogenetic dextranase.…”

An alkaline and surfactant-tolerant lipase from Trichoderma lentiforme ACCC30425 with high application potential in the detergent industry

“…Moreover, Trichoderma has significant lignocellulose-degrading capability because it can produce a variety of hydrolytic, lytic and auxiliary enzymes including cellulase, xylanase, chitinase, laccase, lipase, etc. (Zhang and Xia 2017 ). Besides, Trichoderma represents one of the most important expression systems that is widely used to produce industrial enzymes on large scale (Singh et al 2015 ).…”

Recent advances in bio-based multi-products of agricultural Jerusalem artichoke resources

“…W0 95.5 g/L [ 95 ] Zymomonas mobilis TRSTR548 95.9 g/L [ 129 ] Kluyveromyces cicerisporus 96.3 g/L [ 130 ] Saccharomyces cerevisiae DTN 109.4 g/L [ 131 ] Saccharomyces cerevisiae Z-06 mixed with Aspergillus niger SL-09 154.7 g/L [ 94 ] Biodiesel Chlorella protothecoides using hydrolysate of Jerusalem artichoke 7.1 g/L [ 100 ] Rhodotorula mucilaginosa TJY15a 10.2 g/L [ 132 ] Rhodosporidium toruloides Y4 39.6 g/L [ 102 ] 2,3-Butanediol Paenibacillus polymyxa ZJ-9 36.92 g/L [ 110 ] Bacillus polymyxa ATCC 12321 44 g/L [ 107 ] Klebsiella pneumoniae 91.63 g/L [ 108 ] Bacillus licheniformis ATCC 14580 103.0 g/L [ 105 ] A recombinant Bacillus sp. strain BRC1 with increased inulinase activity 28.6 g/L [ 133 ] …”