A new technique for isolating pathogenic fungi from mixed flora inocula

Smith, Andrew G.; Rohde, Paul A.

doi:10.1007/bf02126158

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1973

1974

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Cited by 3 publications

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“…A variety of media are available for the selective isolation of fungi from clinical specimens (1,4,5,11,15,16). The addition of cycloheximide to fungal growth media will also inhibit numerous saprophytic and some pathogenic fungi (10).…”

mentioning

confidence: 99%

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Smith¹,

Blasi²,

Dayton³

1974

Applied Microbiology

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mentioning

confidence: 99%

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Smith¹,

Blasi²,

Dayton³

1974

Applied Microbiology

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Chapter 13. Antifungal Agents

Waitz¹,

Drube²

1973

Annual Reports in Medicinal Chemistry

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Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Smith¹,

Blasi²,

Dayton³

1974

Appl Microbiol

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Six media were evaluated to determine their ability to isolate yeasts and inhibit bacteria. The media included the following: Snyder, Snyder tellurite, Sabouraud tellurite, Littman-gentamicin, molybdate, and Mycosel (BBL). Doses of mixed intestinal gram-negative bacilli and enterococci were most effectively inhibited by Snyder tellurite agar. Klebsiella pneumoniae was the most common bacterial contaminant of the other media. All six media were comparable in isolating yeasts while preventing the growth of the oral bacterial flora. The selection of a basal fungal growth medium for tellurite incorporation to inhibit bacteria but permit growth of yeasts was affected by pH. The bacteriostatic effect of tellurite was decreased with increasing pH of media while fungistatic action was increased. The arbitrary selection of Snyder and Littman agars to isolate yeast from burn wound cultures demonstrated the need to include a selective medium for these specimens. Blood, phenylethyl alcohol blood agar, and Columbia CN blood agar were all inadequate for isolating yeasts from burns. Growth of a variety of filamentous saprophytic and pathogenic dimorphic fungi grew adequately on four of five selective media tested.

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A new technique for isolating pathogenic fungi from mixed flora inocula

Cited by 3 publications

References 19 publications

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

Chapter 13. Antifungal Agents

Evaluation of Media for Selective Isolation of Yeasts from Oral, Rectal, and Burn Wound Specimens

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