A new system of sperm cryopreservation: evaluation of survival, motility, <scp>DNA</scp> oxidation, and mitochondrial activity

Pabón, D.; Meseguer, Marcos; Sevillano, G.; Cobo, Ana; Romero, J.L.; Remohı́, J.; Santos, María José de los

doi:10.1111/andr.12607

Cited by 35 publications

(29 citation statements)

References 42 publications

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“…It was demonstrated that conventional freezing resulted in higher motility and viability, while spermatozoa undergoing vitrification were healthier regarding morphology and had fewer defects of sperm head, midpiece and tail [32]. Very recently, Pabon et al [33] used 47 human sperm samples to compare the efficiency of vitrification and conventional freezing protocols. They found vitrification was optimal for sperm cryopreservation as vitrification protocol resulted in better motility recovery and higher mitochondrial activity [33].…”

Section: Is Vitrification Superior To Conventional Freezing?mentioning

confidence: 99%

Human sperm vitrification: the state of the art

Tao

Sanger

Saewu

et al. 2020

Reprod Biol Endocrinol

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Sperm cryopreservation has been widely used in assisted reproductive technology (ART) and has resulted in millions of live births. Two principal approaches have been adopted: conventional (slow) freezing and vitrification. As a traditional technique, slow freezing has been successfully employed and widely used at ART clinics whereas the latter, a process to solidify liquid into an amorphous or glassy state, may become a faster alternative method of sperm cryopreservation with significant benefits in regard to simple equipment and applicability to fertility centers. Sperm vitrification has its own limitations. Firstly, small volume of load is usually plunged to liquid nitrogen to achieve high cooling rate, which makes large volume sample cryopreservation less feasible. Secondly, direct contact with liquid nitrogen increases the potential risk of contamination. Recently, new carriers have been developed to facilitate improved control over the volume and speed, and new strategies have been implemented to minimize the contamination risk. In summary, although sperm vitrification has not yet been applied in routine sperm cryopreservation, its potential as a standard procedure is growing.

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Section: Is Vitrification Superior To Conventional Freezing?mentioning

confidence: 99%

Human sperm vitrification: the state of the art

Tao

Sanger

Saewu

et al. 2020

Reprod Biol Endocrinol

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“…Cryopreservation of spermatozoa is an important technology of reproductive medicine [1,2]. Since data on the cryopreservation of human spermatozoa in the presence of cryoprotectants were first published in the late 1950s [3], several cryopreservation methods have been introduced, including conventional freezing and vitrification (cryopreservation by direct plunging into liquid nitrogen) techniques [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…It was noted the high effectiveness of technology for cryoprotectant-free vitrification by direct dropping of human spermatozoa suspension into liquid nitrogen [6,[19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability

Wang¹,

Isachenko²,

Todorov

et al. 2020

BioMed Research International

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This study aimed to compare the quality of human spermatozoa vitrified by direct plunging into liquid nitrogen vs. liquid air. Spermatozoa were divided into three groups: fresh spermatozoa (Group F) were used as a control. Spermatozoa suspension (20 μl) was vitrified in open granules by direct dropping into liquid nitrogen (Group LN) or clean liquid air (Group LA). After warming at 37°C, the progressive motility rate of Group F was reduced from 65.9 ± 2.5% to 34.0 ± 1.9% (Group LN) and 38.1 ± 2.3% (Group LA), respectively (P1-2,3 < 0.05). The reductions in viability were 65.6 ± 2.2%, 29.0 ± 1.8%, and 36.6 ± 2.6% for Groups F, LN, and LA, respectively (P1-2,3 < 0.05). Comparing spermatozoa vitrified in liquid nitrogen vs. liquid air, no significant differences were detected in motility (34.0 ± 1.9% vs. 38.1 ± 2.3%), viability (29.0 ± 1.8% vs. 36.6 ± 2.6%), early apoptosis (13.8 ± 1.5% vs. 14.3 ± 1.8%), late apoptosis (45.5 ± 1.8% vs. 43.7 ± 2.2%), and necrosis (19.5 ± 2.0% vs. 15.0 ± 1.8%; p>0.01 for all respective differences). There was a statistical tendency for increasing rates of “progressive motility” and “viability” and decreasing rates of “apoptosis” and “necrosis” when comparing spermatozoa vitrified in liquid air vs. liquid nitrogen. It is concluded that cryoprotectant-free vitrification by the direct dropping of human spermatozoa in a clean cooling agent (liquid air) is a good alternative to the use of nonsterile liquid nitrogen and can be used to cool cells while minimising the risk of microbial contamination.

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“…Currently, three cryopreservation methods are used including slow freezing, rapid freezing and vitrification. Since slow freezing is time consuming with the need for an expensive programmable freezer, research activities have increasingly been oriented towards the rapid freezing and vitrification methods in recent years [1]- [5]. Rapid freezing and vitrification are also superior to slow freezing in preserving sperm motility and DNA integrity [5]- [10].…”

Section: Introductionmentioning

confidence: 99%

“…Since slow freezing is time consuming with the need for an expensive programmable freezer, research activities have increasingly been oriented towards the rapid freezing and vitrification methods in recent years [1]- [5]. Rapid freezing and vitrification are also superior to slow freezing in preserving sperm motility and DNA integrity [5]- [10]. In particular, rapid freezing and vitrification rely only on non-permeating cryoprotective agents (CPA), including natural sugars such as sucrose and trehalose, for sperm cryopreservation omitting the harmful effects of permeating CPA such as glycerol on human sperm [3] [4] [11] [12].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Rapid Freezing and Vitrification for Human Sperm Cryopreservation Using Trehalose as a Cryoprotective Agent

Hu¹,

Li²,

Shi³

et al. 2019

OJOG

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Rapid freezing and vitrification are becoming popular for human sperm cryopreservation; however, it remains unclear which method is better. The aims of the present study were to determine the optimal trehalose concentration and to compare the cryoprotective effects of rapid freezing and vitrification. The results showed that: 1) The optimal trehalose concentration was 0.25 mol/L; 2) The post-thaw recovery rates of total and progressive sperm motilities after rapid freezing (38.6% ± 3.0% and 41.1% ± 5.0%) were significantly higher (P < 0.05) than that after vitrification (26.1% ± 3.1% and 27.2% ± 1.3%) when 0.5 mL straws were used; 3) However, the recovery rates of total and progressive motilities after rapid freezing in 0.5 mL straw (26.7% ± 9.6% and 26.8% ± 8.7%) were significantly lower (P < 0.05) than that after vitrification in a novel straw-in-straw system (43.1% ± 4.2% and 41.8% ± 15.5%); and 4) The post-thaw sperm nuclear DNA damage level after rapid freezing in 0.5 mL straw (8.7% ± 2.8%) was not significantly different from that of sperm after vitrification in the straw-in-straw system (9.2% ± 2.5%). It was concluded that rapid freezing is superior to vitrification when using 0.5 mL straws; however, vitrification is superior to rapid freezing when using the straw-in-straw systems.

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A new system of sperm cryopreservation: evaluation of survival, motility, DNA oxidation, and mitochondrial activity

Cited by 35 publications

References 42 publications

Human sperm vitrification: the state of the art

Human sperm vitrification: the state of the art

Aseptic Technology for Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into Clean Liquid Air: Apoptosis, Necrosis, Motility, and Viability

Comparison of Rapid Freezing and Vitrification for Human Sperm Cryopreservation Using Trehalose as a Cryoprotective Agent

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