A new system for the treatment of retinal degeneration

Wrobel, Walter-G.; Banzhaf, André; Blaess, Guido; Eipper, Carmen; Harscher, Alex; Hekmat, Anuschirawan; Möller, Andreas; Mohrlok, Rainer; Pätzold, J

doi:10.1515/bmt.2011.106

Cited by 1 publication

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Like TES, WES places an active electrode on the cornea, but the reference electrode is placed in the mouth or elsewhere on the head, permitting the current to become more uniformly distributed throughout the eye (Rahmani et al, 2013). This approach has often been referred to as TES in the literature and can be applied with a DTL electrode in animals and humans (Schatz et al, 2011, 2012) or contact lens in animal models (Willmann et al, 2011; Wrobel et al, 2011). Current magnitudes for TES and WES treatments range from 1.5 to 1000 μA (Pardue et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Whole-eye electrical stimulation therapy preserves visual function and structure in P23H-1 rats

Hanif

Kim

Thomas

et al. 2016

Experimental Eye Research

View full text Add to dashboard Cite

Low-level electrical stimulation to the eye has been shown to be neuroprotective against retinal degeneration in both human and animal subjects, using approaches such as subretinal implants and transcorneal electrical stimulation. In this study, we investigated the benefits of whole-eye electrical stimulation (WES) in a rodent model of retinitis pigmentosa. Transgenic rats with a P23H-1 rhodopsin mutation were treated with 30 min of low-level electrical stimulation (4 μA at 5 Hz; n = 10) or sham stimulation (Sham group; n = 15), twice per week, from 4 to 24 weeks of age. Retinal and visual functions were assessed every 4 weeks using electroretinography and optokinetic tracking, respectively. At the final time point, eyes were enucleated and processed for histology. Separate cohorts were stimulated once for 30 min, and retinal tissue harvested at 1 h and 24 h post-stimulation for real-time PCR detection of growth factors and inflammatory and apoptotic markers. At all time-points after treatment, WES-treated rat eyes exhibited significantly higher spatial frequency thresholds than untreated eyes. Inner retinal function, as measured by ERG oscillatory potentials (OPs), showed significantly improved OP amplitudes at 8 and 12 weeks post-WES compared to Sham eyes. Additionally, while photoreceptor segment and nuclei thicknesses in P23H-1 rats did not change between treatment groups, WES-treated eyes had significantly greater numbers of retinal ganglion cell nuclei than Sham eyes at 20 weeks post-WES. Gene expression levels of brain-derived neurotrophic factor (BDNF), caspase 3, fibroblast growth factor 2 (FGF2), and glutamine synthetase (GS) were significantly higher at 1 h, but not 24 h after WES treatment. Our findings suggest that WES has a beneficial effect on visual function in a rat model of retinal degeneration and that post-receptoral neurons may be particularly responsive to electrical stimulation therapy.

show abstract