A New Swivel Model for Parenteral and Enteral Infusion in Rats

Galizia, Mauricio S.; Alves, Cléber R.; Tamanaha, Erika Midori; Torrinhas, Raquel Susana; Leite, Fernando; Neto, Alberto Hernandez; Gama-Rodrigues, Joaquim; Waitzberg, Dan Linetzky

doi:10.1016/j.jss.2004.12.004

Cited by 11 publications

(10 citation statements)

References 8 publications

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“…Animals were anesthetized with an intraperitoneal injection of ketamine (100 mg/kg of body weight), and parenteral access was achieved by jugular vein cannulation, according to a standard technique, 12 followed by a connection to a swivel apparatus to allow the animals free mobility. 13 All rats were then housed in metabolic cages. All intravenous treatments were delivered with a peristaltic pump (0.5 mL/h rate) for 72 hours.…”

Section: Parenteral Accessmentioning

confidence: 99%

Partial Replacement of ω‐6 Fatty Acids With Medium‐Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis

Bertevello

Nardi

Torrinhas

et al. 2012

J Parenter Enteral Nutr

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Background: Soybean oil is rich in ω‐6 fatty acids, which are associated with higher incidence and more severe cases of inflammatory bowel diseases. The authors evaluated whether partial replacement of soybean oil by medium‐chain triglycerides (MCTs) or olive oil influenced the incidence and severity of experimental ulcerative colitis by using different parenteral lipid emulsions (LEs). Methods: Wistar rats (n = 40) were randomized to receive parenteral infusion of the following LE: 100% soybean oil (SO), 50% MCT mixed with 50% soybean oil (MCT/SO), 80% olive oil mixed with 20% soybean oil (OO/SO), or saline (CC). After 72 hours of infusion, acetic acid experimental colitis was induced. After 24 hours, colon histology and cytokine expression were analyzed. Results: SO was not significantly associated with overall tissue damage. MCT/SO was not associated with necrosis (P < .005), whereas OO/SO had higher frequencies of ulcer and necrosis (P < .005). SO was associated with increased expression of interferon‐γ (P = .005) and OO/SO with increased interleukin (IL)–6 and decreased tumor necrosis factor–α expression (P < .05). MCT/SO appeared to decrease IL‐1 (P < .05) and increase IL‐4 (P < .001) expression. Conclusions: Parenteral SO with high concentration of ω‐6 fatty acids was not associated with greater tissue damage in experimental colitis. SO partial replacement with MCT/SO decreased the frequency of histological necrosis and favorably modulated cytokine expression in the colon; however, replacement with OO/SO had unfavorable effects.

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Section: Parenteral Accessmentioning

confidence: 99%

Partial Replacement of ω‐6 Fatty Acids With Medium‐Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis

Bertevello

Nardi

Torrinhas

et al. 2012

J Parenter Enteral Nutr

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“…In conclusion, the main problems in the usage of the orogastric route can be enumerated as time-consuming processes, sedation requirements in many experiment protocols, two personnels for the handling and the manipulation, need for experience, and the mortality due to tracheal intubation [1,2,[7][8][9][10]. Tube ostomies are frequently used but anesthesia requirement, operative mortality, wound infections, damage to the ostomies of the animals between themselves, limitation in the movements of the animals, and high cost are the disadvantages of these routes.…”

Section: Discussionmentioning

confidence: 99%

“…Application of the research material to the gastrointestinal tract may be available by adding the material to the water of the experiment animal, through an orogastric tube or with a surgically managed ostomy [1,2]. Here in we presented a novel and simple tube gastrostomy model in an experimental rat model in which rats had unrestrained movements.…”

Section: Aimmentioning

confidence: 99%

A New Tube Gastrostomy Model in Animal Experiments

Sezer¹

2013

Ann Clin Anal Med

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ÖzetAmaç: Orogastrik yol, deney hayvanlarına orogastrik tüp veya cerrahi olarak açılan bir osteotomi aracılığıyla su vermek amacıyla sıklıkla kullanılan yoldur. Biz bu çalışmada hareketleri dizginlenmemiş deneysel rat modellerinde yeni ve basit tüp gastrostomi modelini sunmayı amaçladık. Gereç ve Yön-tem: Bu deney on iki erkek Sprague-Dawley ratının rastgele olarak kontrol grubu ( grup C, n: 6) ve tüp gastrostomi grubu ( group TG, n: 6) olarak iki gruba ayrılmasıyla oluşturuldu. Silikon bir foley kateterden(6 French) basit bir gastrostomi tüpü türetildi. TG grubunda bir kesi yapıldı ve mide görüntülen-di. Peritonyum açılışında orta hatta 1 cmlik bir kesi yapıldı. Askı dikişler atıl-dı ve ön gastrik duvar kaldırıldı. Sonrasında gastrik duvar açıldı. Cihaz mideye yerleştirildi ve cilt altından tünel içinden ilerletilip yan karın duvarına 2/0 dikiş ile tespit edildi. Bulgular: Prosedür deneyin onuncu gününde sona erdirildi. Grup C'de mortalite gözlenmedi. Ratlar günlük olarak takip edildi ve herhangi bir kendi kendine zarar verme, ısırık izi, ağızdan beslenmeye direnç göster-me, kaçmaya çalışma gibi anormal davranış gözlenmedi. Deney sonunda TG grubunda alanin transaminaz düzeyindeki artışta (p<0.05) ve vücut ağırlığı ve proteinindeki azalışta (p<0.05) istatistiksel anlamlı farklılık gözlendi. Grup C'de ise üre seviyesindeki artış (p<0.05) istatistiksel olarak anlamlı idi. Aynı zamanda Grup C'deki aspartat transaminaz, albumin, vücut ağırlığı ve total protein seviyesindeki düşüş de (p<0.05) istatistiksel olarak anlamlı idi. Preoperatif dönemden deneyin sonuna kadar olan dönemde gastrostomi ve laparotomi gruplarındaki glikoz (p=0.047) ve aspartat transaminaz (p=0.050) düzeylerindeki düşüş ve tartı kaybı (p=0.034) istatistiksel olarak anlamlı idi.Tartışma: Biz, yeni geliştirdiğimiz tüp gastrostomi modelinin orogastrik yolun kullanılamadığı deneysel modellerde iyi bir alternatif olacağına inanmaktayız. Anahtar KelimelerHayvan Deneyleri; Deneysel Dizayn; Gastrostomi Abstract Aim: The orogastric route is the most preferred application method in the vast majority of the animal experiments in which application can be achieved by adding the material to the water of the experiment animal, through an orogastric tube or with a surgically managed ostomy. Material and Method: This experiment was constructed with twelve male Sprague-Dawley rats which were randomly assigned to one of two groups consist of control group ( group C, n: 6) and tube gastrostomy group ( group TG, n: 6).A novel and simple gastrostomy tube was derivated from a silicone foley catheter. Tube gastrostomy apparatus was constituted with a silicone foley catheter (6 French). In the group TG an incision was performed, and the stomach was visualized. A 1 cm incision was made in the midline and opening of the peritoneum. Anchoring sutures were placed and anterior gastric wall was lifted. The gastric wall is then opened. The apparatus was placed into the stomach and pulled through from a tunnel under the skin and fixed to the lateral abdominal wall with a 2/0 silk...

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“…Intravenous access was achieved by jugular central venous catheterization (CVC), according to a standard technique, followed by connection to a swivel apparatus that allowed the animals to have free mobility [11, 12]. After CVC, all animals received 0.9% saline solution infusion for 24 h. After this period, the animals were randomized to receive 48 h intravenous infusion of 6 mL/day 0.9% saline solution (saline group, n = 44) or 1 g/kg body weight dipeptide alanyl-glutamine (Dipeptiven® 20%, Fresenius-Kabi, Bad Homburg, Germany; glutamine group, n = 42), or no infusion (nontreatment group, n = 45).…”

Section: Methodsmentioning

confidence: 99%

Effect of Previous High Glutamine Infusion on Inflammatory Mediators and Mortality in an Acute Pancreatitis Model

Garib

Garla

Torrinhas

et al. 2016

Mediators of Inflammation

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Parenteral glutamine supplementation in acute inflammatory conditions is controversial. We evaluated the inflammatory and survival responses after parenteral glutamine infusion in sodium taurocholate-induced acute pancreatitis (AP) model. Lewis rats received 1 g/kg parenteral glutamine (n = 42), saline (n = 44), or no treatment (n = 45) for 48 h before AP induction. Blood, lung, and liver samples were collected 2, 12, and 24 h after AP to measure serum cytokines levels and tissue heat shock protein (HSP) expression. From each group, 20 animals were not sacrificed after AP for a 7-day mortality study. Serum cytokine levels did not differ among groups at any time point, but the intragroup analysis over time showed higher interferon-γ only in the nontreatment and saline groups at 2 h (versus 12 and 24 h; both p ≤ 0.05). The glutamine group exhibited greater lung and liver HSP90 expression than did the nontreatment group at 2 and 12 h, respectively; greater liver HSP90 and HSP70 expression than did the saline group at 12 h; and smaller lung HSP70 and liver HSP90 expression than did the nontreatment group at 24 h (all p ≤ 0.019). The 7-day mortality rate did not differ among groups. In experimental AP, pretreatment with parenteral glutamine was safe and improved early inflammatory mediator profiles without affecting mortality.

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A New Swivel Model for Parenteral and Enteral Infusion in Rats

Cited by 11 publications

References 8 publications

Partial Replacement of ω‐6 Fatty Acids With Medium‐Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis

Partial Replacement of ω‐6 Fatty Acids With Medium‐Chain Triglycerides, but Not Olive Oil, Improves Colon Cytokine Response and Damage in Experimental Colitis

A New Tube Gastrostomy Model in Animal Experiments

Effect of Previous High Glutamine Infusion on Inflammatory Mediators and Mortality in an Acute Pancreatitis Model

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