Abstract:To explore the formation, morphological characteristics, cell composition, and differentiation potential of cardiomyocyte annulation (cardio‐annulation) during in vitro culture of cardiac cells. Cardiac cells were isolated and cultured. A live‐cell imaging system was used to observe cardio‐annulation. Cardiac troponin‐T (cTnT) and vimentin were labeled with double immunofluorescence staining, and coexpressions of cTnT and connexin43 (Cx43), cTnT and nanog, c‐kit and nanog, and c‐kit and stem cell antigen (sca‐… Show more
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