A new strategy to construct a FRET platform for ratiometric sensing of hydrogen sulfide

Abstract

We introduce a new FRET strategy to construct a ratiometric fluorescent H2S sensor. The ratio emission signal of the coumarin-naphthalimide dyad is modulated by the FRET process, which works in coordination with the ICT mechanism. The FRET process on/off is controlled through tuning the overlap level of the donor emission spectrum with the acceptor absorption via modulation of the acceptor fluorophore absorption wavelength. was applied to visualize both the intracellular exogenous and endogenous H2S through blue and green emission channels.

Design and synthesis of a novel chromium(III) selective fluorescent chemosensor bearing a thiodiacetamide moiety and two rhodamine B fluorophores

“…Over the past several years, fluorescent chemosensors have been developed as useful sensors for various metal ions due to their simplicity, specificity and sensitivity monitoring with a fast response time [27][28][29]. Therefore, various fluorophores with different excitation and emission wavelengths, such as coumarin [30,31], pyrene [32,33], 1, 8-naphthalimide [34,35], xanthenes [36,37], squaraine [38], cyanine [39], boron dipyrromethene difluoride (BODIPY) [40], and nitrobenzofurazan [41], have been employed to develop various chemical sensors. Among the developed fluorophores, rhodamine and its derivatives [42,43] employ an ideal framework in the construction of fluorescent probes due to their excellent spectroscopic properties including high fluorescence quantum yield, large molar extinction coefficient, and visible wavelength excitation [44][45][46][47].…”

Dual Mechanism of an Intramolecular Charge Transfer (ICT)–FRET‐Based Fluorescent Probe for the Selective Detection of Hydrogen Peroxide

“…It is not easy to build these FRET‐based ratiometric fluorescent platforms because they require considerable spectral overlap between the emission of the donor and the absorption of the acceptor. However, intramolecular charge transfer (ICT) could trigger the absorption redshift and quantum yield increase of the fluorophore . It could be used to change the level of spectral overlap of the FRET‐based fluorescent platforms to switch the FRET process on/off.…”

“…Coumarin and naphthalimide are widely used as fluorophores owing to their high photophysical properties . Furthermore, there is good spectral overlap between the emission of coumarin and the absorption of naphthalimide . To obtain more efficient probes, we changed the overlap level by adjusting the naphthalimide absorption wavelength to exert on/off control over the FRET process.…”

Colorimetric and Fluorometric Discrimination of Geometrical Isomers (Maleic Acid vs Fumaric Acid) with Real-Time Detection of Maleic Acid in Solution and Food Additives

“…Compared to the large number of chromogenic receptors for the sensing of metal ions, [11][12][13] very few chromogenic receptors for anions and small molecules have been developed that are based on recognition approaches. [13][14][15][16] In fact, a thorough literature survey revealed that there are very few colorimetric or fluorogenic sensors which dealt with the use of synthetically constructed receptors in carboxylic acids sensing purpose. [17][18] Supramolecular concepts [19][20] in designing new optical chemosensors has become a very common trend in recent-past based on changes in fluorescence [21][22][23] and in absorbance [24][25][26] as the output signals.…”