A new strategy for the diagnosis of MAGE-expressing cancers

Park, Jong Wook; Kwon, Taeg Kyu; Kim, In Ho; Sohn, Soo Sang; Kim, You Sah; Kim, Chun Il; Bae, Ok Suk; Lee, Kyung Seop; Lee, Kang Dae; Lee, Cheong Sam; Chang, Hee Kyung; Choe, B. K.; Ahn, Sungjin; Jeon, Chang‐Ho

doi:10.1016/s0022-1759(02)00105-9

Cited by 67 publications

(79 citation statements)

References 22 publications

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“…All primers used for amplification steps are summarized in Table 1. High sensitive RT-nested PCR methods (nPCR) were used for the detection of MAGE-A1-A6 (MAGE-A1-6 Assay) and A12 (RT-nPCR) expressing tissues [15,24,25].…”

Section: Detection Of Mage-a Expression By Rt-pcrmentioning

confidence: 99%

“…These primers can bind to the sequences of MAGE-A1, A2, A3, A4, A5 and A-6 together and allow a simultaneous detection of all subtypes by the same method as described previously [15,23]. For nPCR, 1 µl of first PCR product was used as a template for the second PCR.…”

Section: Detection Of Mage-a Expression By Rt-pcrmentioning

confidence: 99%

“…The MAGE-A family consists of 12 subtypes including MAGE-A1 to -A12 [7][8][9][10]. Based on the reverse transcriptase polymerase chain reaction (RT-PCR) typing results for MAGE-A genes, except MAGE-A7 which is not found to be not transcribed, MAGE-A is expressed almost exclusively in various tumor types and testis [9][10][11][12][13][14][15][16][17][18][19]. Expression of single MAGE-A genes has already been extensively examined for the diagnostic application of different malignancies.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, expression frequency was higher when multiple MAGE-A was applied. Previous studies reported that multiple MAGE-A analysis does increase the sensitivity of tumor cell detection in solid tumors including OSCC compared to single MAGE-A analysis [15][16][17][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…There are several reports regarding to establishment of molecular diagnostic techniques for the early diagnosis of primary tumors, and also detection of rare occult tumor cells or DTC's using multiple molecular markers with definitive accuracy to malignancies including the one based on MAGE-A expression pattern [15,[23][24][25][26][27][28]. Therefore, in order to increase the sensitivity of the previously established methods, it was aimed to investigate the expression rate of mRNA transcripts of 10 different MAGE-A subtypes using RT-PCR in a large number of OSCC samples to determine whether multiple expression analysis of MAGE-A is a more sensitive application for the detection of OSCC.…”

Section: Introductionmentioning

confidence: 99%

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A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

et al. 2009

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Abstract. Objective: Melanoma associated antigens-A (MAGE-A) expression is highly specific to cancer cells. Thus, they can be the most suitable targets for the diagnosis of malignancy. The aim of this study was to evaluate the sensitivity of multiple MAGE-A expression analysis for the diagnosis of oral squamous cell carcinoma (OSCC). Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of otherwise healthy volunteers were examined for the expression of 10 different single antigens out of 12 different MAGE-A subtypes by highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) methods. The results were correlated to clinicopathological parameters of tumor samples. Results: Expression of MAGE-A was restricted to OSCC. The expression frequency of single antigen was between 10% and 55%. However, expression rate was increased up to 93% by the elevated number of genes examined. A significant correlation was found between the expression of MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A detection has also correlated to the incidence of lymph node metastasis, grading and advanced clinical stages. Conclusions: Analysis of multiple MAGE-A expression is more sensitive than the analysis of a single MAGE-A for the diagnostic evaluation of OSCC. Multiple MAGE-A expression analysis may be a very sensitive method to be used for the diagnosis even in the early stage of OSCC.

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Section: Detection Of Mage-a Expression By Rt-pcrmentioning

confidence: 99%

Section: Detection Of Mage-a Expression By Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

et al. 2009

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The melanoma antigen gene as a surveillance marker for the detection of circulating tumor cells in patients with breast carcinoma

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Kang

et al. 2005

Cancer

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Modified internucleotide linkage featuring the C3′‐O‐P‐CH2‐O‐C4″ phosphonate grouping as an isosteric alternative to the phosphodiester C3′‐O‐P‐O‐CH2‐C4″ bond was studied in order to learn more on its stereochemical arrangement, which we showed earlier to be of prime importance for the properties of the respective oligonucleotide analogues. Two approaches were pursued: First, the attempt to prepare the model dinucleoside phosphonate with 13C‐labeled CH2 group present in the modified internucleotide linkage that would allow for a more detailed evaluation of the linkage conformation by NMR spectroscopy. Second, the use of ab initio calculations along with molecular dynamics (MD) simulations in order to observe the most populated conformations and specify main structural elements governing the conformational preferences. To deal with the former aim, a novel synthesis of key labeled reagent (CH3O)2P(O)13CH2OH for dimer preparation had to be elaborated using aqueous 13C‐formaldehyde. The results from both approaches were compared and found consistent. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 514–529, 2009. This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Expression of cancer testis antigens in head and neck squamous cell carcinomas

et al. 2006

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We conclude that the tumor-specific antigen genes are expressed in variable frequencies and intensities in the primary lesions of head and neck squamous cell carcinomas and in their metastases, with expression of the PRAME gene being always present in the metastastatic lymph nodes. In primary lesions, gene expression correlated with smoking habit and with advanced tumors with a higher malignant potential, with the frequent expression of two or more of these genes.

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A new strategy for the diagnosis of MAGE-expressing cancers

Cited by 67 publications

References 22 publications

A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma

The melanoma antigen gene as a surveillance marker for the detection of circulating tumor cells in patients with breast carcinoma

Expression of cancer testis antigens in head and neck squamous cell carcinomas

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