A new strain of tomato severe leaf curl virus and a unique variant of tomato yellow leaf curl virus from Mexico

Bañuelos-Hernández, Bernardo; Mauricio-Castillo, J. A.; Cárdenas-Conejo, Yair; Guevara-González, Ramón Gerardo; Argüello‐Astorga, Gerardo R.

doi:10.1007/s00705-012-1358-z

Cited by 13 publications

(5 citation statements)

References 29 publications

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“… Localization of mutations found in the Intergenic region (IR) of begomovirus quasispecies. (A) Schematic representation of the intergenic region of tomato yellow leaf curl-like viruses showing the location of the replication associated (Rep) and the transcription activator (TrAP) protein binding regions, TATA boxes and ERE-like motifs (Bañuelos-Hernández et al, 2012 ). The nonanucleotide sequence conserved in all geminiviruses and the cleavage site for the initiation of rolling-circle replication in the stem-loop are also shown.…”

Section: Discussionmentioning

confidence: 99%

Differential Shape of Geminivirus Mutant Spectra Across Cultivated and Wild Hosts With Invariant Viral Consensus Sequences

et al. 2018

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Geminiviruses (family Geminiviridae) possess single-stranded circular DNA genomes that are replicated by cellular polymerases in plant host cell nuclei. In their hosts, geminivirus populations behave as ensembles of mutant and recombinant genomes, known as viral quasispecies. This favors the emergence of new geminiviruses with altered host range, facilitating new or more severe diseases or overcoming resistance traits. In warm and temperate areas several whitefly-transmitted geminiviruses of the genus Begomovirus cause the tomato yellow leaf curl disease (TYLCD) with significant economic consequences. TYLCD is frequently controlled in commercial tomatoes by using the dominant Ty-1 resistance gene. Over a 45 day period we have studied the diversification of three begomoviruses causing TYLCD: tomato yellow leaf curl virus (TYLCV), tomato yellow leaf curl Sardinia virus (TYLCSV) and tomato yellow leaf curl Malaga virus (TYLCMaV, a natural recombinant between TYLCV and TYLCSV). Viral quasispecies resulting from inoculation of geminivirus infectious clones were examined in plants of susceptible tomato (ty-1/ty-1), heterozygous resistant tomato (Ty-1/ty-1), common bean, and the wild reservoir Solanum nigrum. Differences in virus fitness across hosts were observed while viral consensus sequences remained invariant. However, the complexity and heterogeneity of the quasispecies were high, especially in common bean and the wild host. Interestingly, the presence or absence of the Ty-1 allele in tomato did not lead to differences in begomovirus mutant spectra. However, the fitness decrease of TYLCSV and TYLCV in tomato at 45 dpi might be related to an increase in CP (Coat protein) mutation frequency. In Solanum nigrum the recombinant TYLCMaV, which showed lower fitness than TYLCSV, at 45 dpi actively explored Rep (Replication associated protein) ORF but not the overlapping C4. Our results underline the importance of begomovirus mutant spectra during infections. This is especially relevant in the wild reservoir of the viruses, which has the potential to maintain highly diverse mutant spectra without modifying their consensus sequences.

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Section: Discussionmentioning

confidence: 99%

Differential Shape of Geminivirus Mutant Spectra Across Cultivated and Wild Hosts With Invariant Viral Consensus Sequences

et al. 2018

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“…From there, TYLCV quickly spread to the USA, where it was identified in Virginia, Tennessee and South Carolina in the mid1990s (Polston et al, 1995), then in Florida (Polston and Anderson, 1997), Georgia (Momol et al, 1999), Mississippi (Ingram and Henn, 2001) and in Central America (Mexico) (Ascencio-Ibáñez et al, 1999;Banuelos-Hernandez et al, 2012;Brown and Idris, 2006). Other independent introductions of TYLCV into the Caribbean likely occurred when TYLCV-IL moved from East-Asia between 2006 and 2011 (Figs.…”

Section: Considered (Summarised Inmentioning

confidence: 98%

Ongoing geographical spread of Tomato yellow leaf curl virus

et al. 2016

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“…The same could be explained in regard to the isolation of Sinaloa‐Mexico (access number FJ012358). As mentioned by Bañuelos‐Hernández et al (2012), there are different introductions of the virus in Mexico: the first one was detected in the Yucatan peninsula (Ascencio‐Ibáñez et al, 1999) and later in the state of Sinaloa (Brown & Idris, 2006; Gámez‐Jiménez et al, 2009). The Sinaloa isolate is associated with infections from California in the United States, which also come from a migration of TYLCV‐IL from Australia (Duffy & Holmes, 2007; Lefeuvre et al, 2010).…”

Section: Discussionmentioning

confidence: 96%

Begomovirus diversity in tomato crops in Costa Rica

Valverde‐Méndez,

Hernández,

Matamoros

et al. 2023

Annals of Applied Biology

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Begomoviruses (Geminiviridae family) are characterized by their high recombination rate and a wide range of hosts, making their control difficult. In Costa Rica, various species of bipartite begomoviruses have been reported, which are Pepper golden mosaic virus (PepGMV), Tomato yellow mottle virus (ToYMoV), Tomato leaf curl Sinaloa virus (ToLCSiV) and the monopartite begomovirus Tomato yellow leaf curl virus (TYLCV). Since the TYLCV first report in Costa Rica, neither additional knowledge has been produced on how this begomovirus has spread in the country's territory nor on the distribution of the other bipartite species. A total of 429 tomato samples collected during the years 2015–2016 were used to study these aspects. Each sample was georeferenced and analysed with various techniques such as nucleic acid hybridization, polymerase chain reaction (PCR) and sequencing for the begomoviruses previously reported in Costa Rica. It was found that the presence/absence of the different species can vary, depending on the province. TYLCV is present in the six provinces analysed in this work, with a proportion from 3.7 to 86.6 per cent. Alajuela, Cartago, and Heredia are the provinces most affected by tomato‐infecting begomoviruses. Fourteen different haplotypes of TYLCV were detected, but all were identified as TYLCV‐IL. The distribution of TYLCV was related to the presence of the whitefly Bemisia tabaci MED, especially in the country's main tomato production areas. This information allows the phytosanitary surveillance services to develop strategies for the integrated management of the disease and to contribute data to the genetic improvement programmes of the crop.

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A new strain of tomato severe leaf curl virus and a unique variant of tomato yellow leaf curl virus from Mexico

Cited by 13 publications

References 29 publications

Differential Shape of Geminivirus Mutant Spectra Across Cultivated and Wild Hosts With Invariant Viral Consensus Sequences

Differential Shape of Geminivirus Mutant Spectra Across Cultivated and Wild Hosts With Invariant Viral Consensus Sequences

Ongoing geographical spread of Tomato yellow leaf curl virus

Begomovirus diversity in tomato crops in Costa Rica

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