A new stonefly species (Plecoptera, Perlidae) from the Interior Highlands USA, with morphological and molecular comparison to other congeneric species

South, Eric J.; DeWalt, R. Edward; Davis, Mark A.; Thomas, M. Jared

doi:10.3897/zookeys.858.33818

Cited by 2 publications

(2 citation statements)

References 17 publications

(26 reference statements)

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“…So far, sequencing of the COI gene fragment has been used to elucidate the systematics and phylogeography of Plecoptera ( Fochetti et al, 2009 ; Fochetti et al, 2011 ; Weiss, Stradner & Graf, 2011 ), and identify new species ( Boumans & Murányi, 2014 ; Graf, Pauls & Vitecek, 2018 ; Pelingen & Freitag, 2020 ; South et al, 2019 ) as part of DNA barcoding initiatives ( Morinière et al, 2017 ; Gattolliat et al, 2016 ; Ferreira et al, 2020 ) and revisionary systematics ( Fochetti et al, 2011 ). Distinct DNA lineages obtained within morphospecies indicate the need for re-examination of morphological characters ( Muster & Michalik, 2020 ; Wachter et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

DNA barcoding for biodiversity assessment: Croatian stoneflies (Insecta: Plecoptera)

Hlebec

Sivec

Podnar

et al. 2022

PeerJ

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Background The hemi-metabolous aquatic order Plecoptera (stoneflies) constitutes an indispensable part of terrestrial and aquatic food webs due to their specific life cycle and habitat requirements. Stoneflies are considered one of the most sensitive groups to environmental changes in freshwater ecosystems and anthropogenic changes have caused range contraction of many species. Given the critical threat to stoneflies, the study of their distribution, morphological variability and genetic diversity should be one of the priorities in conservation biology. However, some aspects about stoneflies, especially a fully resolved phylogeny and their patterns of distribution are not well known. A study that includes comprehensive field research and combines morphological and molecular identification of stoneflies has not been conducted in Croatia so far. Thus, the major aim of this study was to regenerate a comprehensive and taxonomically well-curated DNA barcode database for Croatian stoneflies, to highlight the morphological variability obtained for several species and to elucidate results in light of recent taxonomy. Methods A morphological examination of adult specimens was made using basic characteristics for distinguishing species: terminalia in males and females, head and pronotum patterns, penial morphology, and egg structures. DNA barcoding was applied to many specimens to help circumscribe known species, identify cryptic or yet undescribed species, and to construct a preliminary phylogeny for Croatian stoneflies. Results Sequences (658 bp in length) of 74 morphospecies from all families present in Croatia were recovered from 87% of the analysed specimens (355 of 410), with one partial sequence of 605 bp in length for Capnopsis schilleri balcanica Zwick, 1984. A total of 84% morphological species could be unambiguously identified using COI sequences. Species delineation methods confirmed the existence of five deeply divergent genetic lineages, with monophyletic origin, which also differ morphologically from their congeners and represent distinct entities. BIN (Barcode Index Number) assignment and species delineation methods clustered COI sequences into different numbers of operational taxonomic units (OTUs). ASAP delimited 76 putative species and achieved a maximum match score with morphology (97%). ABGD resulted in 62 and mPTP in 61 OTUs, indicating a more conservative approach. Most BINs were congruent with traditionally recognized species. Deep intraspecific genetic divergences in some clades highlighted the need for taxonomic revision in several species-complexes and species-groups. Research has yielded the first molecular characterization of nine species, with most having restricted distributions and confirmed the existence of several species which had been declared extinct regionally.

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Section: Discussionmentioning

confidence: 99%

DNA barcoding for biodiversity assessment: Croatian stoneflies (Insecta: Plecoptera)

Hlebec

Sivec

Podnar

et al. 2022

PeerJ

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“…Furthermore, the use of an integrative approach could help to solve the problem of delimiting several closely related Perlidae species since some variations/species are difficult to delimit using only morphological adult male characters. In addition to morphology, molecular tools have been used successfully to study stoneflies around the world, mainly to associate life stages [47][48][49], identify cryptic species problems [50], delimit species [51], and address phylogenetic and biogeographic questions [52][53][54][55][56][57][58][59]. In Brazil, molecular tools have been mainly used to successfully associate the nymphs of Plecoptera [21,24,38].…”

Section: Introductionmentioning

confidence: 99%

Perlidae (Plecoptera) from the Paranapiacaba Mountains, Atlantic Forest, Brazil: Diversity and implications of the integrative approach and teneral specimens on taxonomy

Almeida

Bispo

2020

PLoS ONE

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The study of complementary sources of biological variation (e.g. morphological, molecular) has allowed a better understanding of biodiversity through the construction of an integrative taxonomy. Using this approach, specimens from the Paranapiacaba Mountains, southeastern Brazil, were studied to update the knowledge on the stonefly family Perlidae from the region, characterize the species, and make associations between nymphs and adults using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene. The study also discusses the implications of integrative taxonomy and teneral specimens for the study of South American Perlidae. The molecular data were analyzed using Bayesian inference, Neighbor-joining, and delimiting species methods. Our results revealed that, in general, there was a morphological and molecular congruence between species. In the Paranapiacaba Mountains, three genera and 15 species were recorded: Anacroneuria boraceiensis Froehlich 2004, A. debilis (Pictet 1841) (new record), A. fiorentini De Ribeiro and Froehlich 2007 (new record), A. flintorum Froehlich 2002, A. iporanga Bispo and Froehlich 2004, A. itajaimirim Bispo and Froehlich 2004, A. polita (Burmeister 1913), A. subcostalis Klapálek 1921, A. tupi Bispo and Froehlich 2004 (with a description of the nymph), Kempnyia auberti Froehlich 1996, K. colossica (Navás 1934), K. flava Klapálek 1916, K. neotropica (Jacobson and Bianchi 1905) (including its new junior synonym K. petersorum Froehlich 1996), Kempnyia sp., and Macrogynoplax veneranda Froehlich 1984. COI sequences were obtained for 11 species, five of which had nymphs associated with adults. Among the five associated nymphs, the nymph of A. tupi is described here. The results of this study indicate that the color of adult teneral specimens differs from that of mature specimens. Given this, the synonym of K. neotropica and K. petersorum was proposed since these species have high morphological and molecular similarities and differ only in color patterns. In addition, the previous record of A. petersi Froehlich 2002 from the Paranapiacaba Mountains was invalidated since it was considered a teneral specimen of A. flintorum. These results suggest that the development of an integrative taxonomy is essential to continue advancing the study of Perlidae diversity in South America.

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A new stonefly species (Plecoptera, Perlidae) from the Interior Highlands USA, with morphological and molecular comparison to other congeneric species

Cited by 2 publications

References 17 publications

DNA barcoding for biodiversity assessment: Croatian stoneflies (Insecta: Plecoptera)

DNA barcoding for biodiversity assessment: Croatian stoneflies (Insecta: Plecoptera)

Perlidae (Plecoptera) from the Paranapiacaba Mountains, Atlantic Forest, Brazil: Diversity and implications of the integrative approach and teneral specimens on taxonomy

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