A New Stability Indicating Validated RP-HPLC Method for Simultaneous Estimation of Escitalopram and Clonazepam in Bulk and Tablet Dosage Form

Mondal, Prasenjit; Kola, Venu

doi:10.5958/2231-5675.2019.00032.2

Cited by 12 publications

(8 citation statements)

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“…The limit of detection was defined as the concentration for which a signal-to-noise ratio of 3 was obtained and for quantitation limit, a signal-to-noise ratio of 10 was considered. [ 22 ] standard solution of darunavir was prepared by sequential dilution and injected into the chromatographic system in decreasing order of concentration in the range of 0.01–10 µg/mL of darunavir.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

A Novel Ultra Performance Liquid Chromatography-PDA Method Development and Validation for Darunavir in Bulk and Its Application to Marketed Dosage Form

Biswal

Mondal

Mondal³

2021

Journal of Pharmacy and Bioallied Sciences

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A BSTRACT Aims and Objective: The aim of this study was to develop and validate a novel ultra-performance liquid chromatographic method for estimation of darunavir in a bulk and tablet dosage form. Materials and Methods: The chromatographic separation was achieved using DIKMA Endoversil (2.1mm x 50mm, 1.7 µm) column. A mixture of 40% buffer (0.1% octa sulfonic acid) and 60% acetonitrile was used as a mobile phase with the isocratic elution mode and eluent was monitored at 281nm using UV detector. The method was continued and validated in accordance with International Conference on Harmonization Guidelines. Validation study revealed the specificity and reliability of the method. Results: In this method, darunavir was eluted with retention time of 0.516 min. Calibration curve plots were found linear over the concentration ranges 10–50 μg/mL for darunavir. Limit of detection was 0.02 μg/mL and limit of quantification was found 0.07 μg/mL. The present method was also found stable in force degradation study. Conclusion: The empirical evidences of all the study results revealed the suitability of the estimation of darunavir in bulk and tablet dosage form without any interference from the excipients.

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Section: Aterials and M Ethodsmentioning

confidence: 99%

A Novel Ultra Performance Liquid Chromatography-PDA Method Development and Validation for Darunavir in Bulk and Its Application to Marketed Dosage Form

Biswal

Mondal

Mondal³

2021

Journal of Pharmacy and Bioallied Sciences

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“…To achieve the various levels, different quantities of standard metformin and repaglinide were added to the combined tablet sample solution at a fixed concentration. This study [20] was repeated three times, with the percentage recovery and percentage mean recovery measured each time.…”

Section: Accuracymentioning

confidence: 99%

“…The limit of detection was define as the concentration for which a signal-to-noise ratio of 3 was obtained and for Quantitation limit, a signal-to-noise ratio of 10 was considered [22]. Standard solutions of metformin and repaglinide was prepared as described earlier.…”

Section: Detection and Quantitation Limitmentioning

confidence: 99%

“…By systematically adjusting the chromatographic conditions, the robustness of the established method was investigated [22]. Six sample solutions of metformin and repaglinide were prepared and analysed in triplicate under optimised conditions, with analytical conditions such as flow rate, mobile phase ratio, and detection wavelength varied at three different levels.…”

Section: Robustnessmentioning

confidence: 99%

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Development and Validation of Novel Ultra Performance Liquid Chromatography Technique for the Simultaneous Determination of Metformin and Repaglinide in Bulk and Formulation

Kumar¹,

Acharyya

Patra³

2021

JPRI

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A novel ultra-performance liquid chromatographic technique for the estimation of metformin and repaglinide in a API and tablet dosage form. The chromatographic separation was achieved using DIKMA Endoversil (2.1 x 50mm, 1.7µm) column with a mobile phase of phosphate buffer, pH 4.2 and methanol as a mobile phase (38:62) with a flow rate of 0.3 mL/min and the detection wavelength was monitored at 241 nm. The method was validated in accordance with International conference on harmonization guidelines. In this present method metformin was eleued at 0.516 minute and repaglinide was eluted at 1.152 min. Limit of detection was 0.05 μg/ml for metformin and1.152 μg/ml for repaglinide limit of quantification was found 0.5 μg/mL. Calibration curve plots were found linear over the concentration ranges 1-50 μg/mL for both the analytes. The % assay of the marketed dosage form was found 99.45 % for metformin and 97.08 % for repaglinide. The present study approach was found to be effective in the analysis of both analytes in force degradation conditions, because both the analytes has been specifically eluted in presence of other chromatograms. The experiential evidences of all the study results revealed the suitability of the estimation of metformin and repaglinide in API and tablet dosage form.

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“…There are number of analytical methods for the analysis of pharmaceutical drugs from different formulations . Literature survey revealed various analytical methods have been reported for estimation of ESC alone and in combination with other drugs [28][29][30][31][32][33][34]. Similarly, there are also various methods for determination of FLU alone and in combination with other drugs [35][36].…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC method for simultaneous determination of escitalopram oxalate and flupentixol HCl in tablet dosage form

Panchale¹,

Nimbokar²,

Gudalwar³

et al. 2021

GSC Biol. and Pharm. Sci.

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RP-HPLC method was developed for simultaneous determination of escitalopram oxalate (ESC) and flupentixol HCl (FLU) in tablet dosage form. Mobile phase consisting of mixture of acetonitrile and potassium phosphate buffer (pH 7.0 with 0.1% triethylamine) in the ratio 60: 40 at flow rate of 1ml/min using C18 Grace (250mmX 4.6mm) column at 231 nm. The retention time of ESC with FLU was found to be 2.96 min and 6.98 min, respectively. The linearity range for ESC with FLU observed was 5-25 µg/ml and 10-50 µg/ml, respectively. Method was validated as per ICH guidelines. Validation parameters studied were linearity and range, recovery study, precision, LOD, LOQ and robustness. Statistical data obtained was found to satisfactorily.

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A New Stability Indicating Validated RP-HPLC Method for Simultaneous Estimation of Escitalopram and Clonazepam in Bulk and Tablet Dosage Form

Cited by 12 publications

References 0 publications

A Novel Ultra Performance Liquid Chromatography-PDA Method Development and Validation for Darunavir in Bulk and Its Application to Marketed Dosage Form

A Novel Ultra Performance Liquid Chromatography-PDA Method Development and Validation for Darunavir in Bulk and Its Application to Marketed Dosage Form

Development and Validation of Novel Ultra Performance Liquid Chromatography Technique for the Simultaneous Determination of Metformin and Repaglinide in Bulk and Formulation

RP-HPLC method for simultaneous determination of escitalopram oxalate and flupentixol HCl in tablet dosage form

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