A new speculum electrode for electroretinography

Lachapelle, Pierre; Blain, Lucie

doi:10.1016/0165-0270(90)90147-8

Cited by 14 publications

(7 citation statements)

References 8 publications

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“…Briefly, after a period of dark adaptation of 12 h and under a dim-red light illumination, the rats were anaesthetized with an intramuscular injection of ketamine (85 mg/kg) and xylazine (5 mg/kg) and the pupils were dilated with drops of 1% cyclopentolate hydrochloride. The rats were then placed, lying on their sides, in a modified recording chamber of our design [28] in which the top part housed the rod desensitizing background light and the photostimulator (model PS 22, Grass Instruments, Quincy, MA). A DTL fibre electrode (27/7 X-StaticÒ silver coated conductive nylon yarn: Sauquoit Industries, Scranton, PA, USA) was used as the active electrode [29].…”

Section: Animalsmentioning

confidence: 99%

Structural and functional consequences of bright light exposure on the retina of neonatal rats

et al. 2006

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In a previous study we showed that juvenile rats exposed, for various durations of time, to a bright luminous environment between P14 (eye opening) and P34 developed a light-induced retinopathy (LIR), the severity of which depending on the duration of exposure as well as the age of the rat at the onset of exposure. Our study also revealed that the severity of the LIR increased as the time elapsed between the cessation of exposure and the structural/functional evaluation increased, suggesting that the LIR degenerative process proceeded in two distinct steps namely, an initial (rapid) acute phase that was followed by a (slower) chronic phase. In view of the above, the purpose of the present study was to reinvestigate previous claims suggesting that exposure to bright light prior to eyelid opening had no measurable consequences on the retinal structure and function; the claim being that despite a non-detectable acute phase, bright light exposure prior to eyelid opening could nonetheless yield a significant retinopathy during the chronic phase of development of LIR. In order to test our hypothesis, neonatal rats were raised in a bright luminous environment from birth to P14. At P30, analysis of the results obtained from rats exposed between P0-P14 did not reveal, as previously acknowledged by others, significant LIR damages. However, results obtained at P60 disclosed significant functional anomalies with relative sparing of the retinal ultrastructure. Our results confirm that, in spite of closed eyelids, postnatal exposure to bright environment did trigger a slow degenerative process.

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Section: Animalsmentioning

confidence: 99%

Structural and functional consequences of bright light exposure on the retina of neonatal rats

et al. 2006

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“…For example, for the photopic ERG the largest ERGs (a-and b-waves) were obtained from 5-day-old specimens and the amplitude of the b-wave at P1 is not significantly different from that measured at P30 or beyond. As indicated in the method section, our recording system [35] is quite different from that used elsewhere; the animal being literally housed in a small (light-proof and almost airtight) ganzfeld (L: 60 cm, W: 50 cm, H: 22 cm) and not in an open environment, something that would definitively accelerated body heat exchange. The animals lie on their side facing the light source (background and flash).…”

Section: Discussionmentioning

confidence: 99%

“…The guinea pigs were then placed in a recording box (ganzfeld-like) of our own design [35], which also housed the flash stimulator and background light. Throughout the recording session the temperature inside the recording box remained at 28°C.…”

Section: Erg Recordingsmentioning

confidence: 99%

Structural and functional maturation of the retina of the albino Hartley guinea pig

2007

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“…Animals also had their pupils dilated with 2 drops of 1% cyclopentolate hydrochloride. The guinea pigs were then placed in a modified recording box (ganzfeld-like) of our own design, which also housed the flash stimulator and background light [20]. Throughout the recording session, the temperature inside the recording box was maintained at 23°C.…”

Section: Electroretinogram Recordingsmentioning

confidence: 99%

Longitudinal assessment of retinal structure and function reveals a rod-cone degeneration in a guinea pig model initially presented as night blind

2011

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We have previously reported a naturally occurring retinopathy in a population of guinea pigs, where the affected animals presented a defect of the rod-mediated vision. The purpose of this study was to investigate if the mutants were affected with a stationary or degenerative retinopathy and to identify the cellular origin of this unique disorder. Electroretinogram (ERG) [postnatal day 1 (P1) to P450], light (LM) and electron microscopy (EM) [P5, P150, P450], and immunohistochemistry [P30, P150, P450] were evaluated from normal and mutant animals. Irrespective of age, the scotopic ERGs of mutants could only be evoked by bright flashes, and the resulting ERGs were of photopic waveform. Interestingly, the amplitude of the cone and the rod/cone a-waves was always of smaller amplitude in mutants, but this difference tended to decrease with age. In contrast, the b-waves were of larger amplitude than normal in photopic ERGs obtained prior to age 25 (days) and prior to age 10 for rod/cone ERGs. LM revealed, in mutants, an absence of the outer segment layer (OSL) with a reduction in the outer nuclear layer (ONL) thickness. EM disclosed the presence of cone outer segment (OS) while no rod OS could be evidenced. Immunohistochemistry revealed the presence of rhodopsin, both cone opsins as well as normal synaptophysin immunoreactivity. Finally, neither the retinal structure nor the function in the mutants achieved normal development. Results suggest that mutant animals are suffering from a degenerative retinal disorder that affects the structure and function of rods and cones.

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A new speculum electrode for electroretinography

Cited by 14 publications

References 8 publications

Structural and functional consequences of bright light exposure on the retina of neonatal rats

Structural and functional consequences of bright light exposure on the retina of neonatal rats

Structural and functional maturation of the retina of the albino Hartley guinea pig

Longitudinal assessment of retinal structure and function reveals a rod-cone degeneration in a guinea pig model initially presented as night blind

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