A new species of the genusBullera Derx

Ninety-nine strains of ballistospore-forming yeasts were isolated from 43 samples of dead leaves and stems of the Japanese rice plant (Oryza sativa L.), and identified as belonging to the genera Bullera, Sporobolomyces, and Tilletiopsis. Thirty-one strains out of 55 of Bullera were assigned to B. alba (15 strains), B. crocea (13 strains), and B. piricola (3 strains). The remaining 24 strains of this genus were divided into 6 groups (species) which could not be assigned to any hitherto known species. Thirty-nine strains out of 41 of Sporobolomyces were assigned to S. roseus (35 strains) and S. shibatarrus (4 strains). The remaining 2 strains of this genus were divided into 2 groups (species) which could not be assigned to any hitherto described species. Three strains of Tilletiopsis were assigned to T. lilacina. Of the 8 undescribed species, two were described respectively as the new species, B. oryzae and S. subbrunneus. Strains JCM 5281 and JCM 5278 respectively were designated as the types of the two species. Further study of the remaining 6 undescribed species is required before we determine their taxonomic position. The frequency of isolation of ballistosporeforming yeasts from Oryza sativa L. was 86°c. Sporobolomyces roseus was found in the highest frequency, 72 %, followed by Bullera sp. 1 (35 %), B. crocea (300) , and B. alba (28 %). The remaining species were found in relatively low frequencies below 9 %. Ballistospore-forming yeasts are known as one of the most common yeasts. They are often found on the surface of various kinds of plants. Since the description of Torula shibatana and Pseudomonilia rubicundula by OKUNUKI (1), several investigations of the classification and distribution of these yeasts have been carried out in Japan (2-5). YAMASAKI and Full (2) described 5 new species of ballistospore-forming yeasts; 4 in Sporobolomyces and 1 in Bu//era. However, three of these Sporobolomyces species were re-identified as known species (6) and the culture of Bullera (B. stramina) was lost. Sporobolomyces coralliformis (4) 457

Section: And Discussionmentioning

confidence: 56%

Ballistospore-forming yeasts found on the surface of the Japanese rice plant, Oryza sativa L.

Nakase

Suzuki

1985

“…We reported a strain from a dead leaf of Oryza sativa (1) with characteristics similar to the present 6 isolates, and described it as a new species in the genus Bullera because it assimilated 2-keto-and 5-ketogluconic acids (3). Assimilation of these compounds is not common in the genus Sporobolomyces but it is common in the genus Bullera as pointed out by STADELMANN (19). We assumed that this species may be a yeast phase of Itersonilia species since strains of Itersonilia were reported to have Q-9 as the major coenzyme Q system (ubiquinone) (16).…”

Section: Tilletiopsis Lilacina Tubakimentioning

confidence: 92%

Studies on ballistospore-forming yeasts from the dead leaves of Miscanthus sinensis with descriptions of the new species Sporobolomyces miscanthi, Sporobolomyces subroseus, and Sporobolomyces weijmanii.

Nakase

Suzuki

1987

One hundred and one strains of ballistospore-forming yeasts isolated from 48 samples of dead leaves of a weed, Miscanthus sinensis, were identified as 16 species in the genera Bullera, Sporobolomyces, and Tilletiopsis: Forty-six of 51 strains of Bullera were assigned to B. megalospora (18 strains), B, crocea (15 strains), B. alba (3 strains), B. piricola (3 strains), B. oryzae (3 strains), B. pseudoalba (3 strains), and B. derxii (1 strain). Five strains resembled unidentified yeasts isolated from the dead leaves of Oryza saliva. Forty-one of 47 strains of Sporobolomyces were assigned to Sp. roseus (37 strains), Sp. salmonicolor (2 strains), and Sp, shibatanus (2 strains). Four strains represented 3 new species and were described as Sp. miscanthi (2 strains), Sp. subroseus (1 strain), and Sp. weijmanii (1 strain). These new species had Q-9 as the major ubiquinone. The remaining 2 strains of Sporobolomyces represent a single species, still unidentified. Three strains of Tilletiopsis were assigned to T. lilacina. The frequency of isolation of each species from Miscanthus sinensis is compared with that from Oryza sativa.In a preceding paper (1), we reported the isolation and identification of ballistospore-forming yeasts living on the surface of dead leaves of the Japanese rice plant, Oryza sativa, collected during the period of winter to early spring. Ballistospore-forming yeasts were found on 86% of the samples examined and were considered to be common inhabitants of this plant. Further, seven new species of ballistospore-forming yeasts were described based on the isolates from Oryza

“…S. antarcticus YK 425 produced a peculiar pattern for FA and 6PGDH which was dissimilar to those of other Sporobolomyces species. According to STADELMANN (27), this species is probably identical with Candida edax. In this study, no activity of FA, 6PGDH, and Cat was detected in C. edax YK 513, and these two strains differed in the pattern of Fma, MDH, G6PDH, HK, and PGM.…”

Section: Discussionmentioning

confidence: 96%

“…The formation of ballistospores was reported by AHEARN et al (24) and YARROW (25), therefore, it was reclassified in the genus Sporobolomyces (26). STADELMANN (27) speculated that S. puniceus and S. singularis should be transferred from the genus Sporobolomyces to the genus Bullera considering their assimilation patterns of carbon compounds. Moreover, FIASON (28) stated that the pale pink color of S. puniceus was not due to carotenoids and suggested that this species might be included in the genus Bullera.…”

Section: Discussionmentioning

confidence: 98%

An electrophoretic comparison of enzymes of ballistosporogenous yeasts.

Yamazaki

Komagata

1983

The patterns produced by nine enzymes of ballistosporogenous yeasts and supposedly related yeasts were studied by electrophoresis on polyacrylamide gel. Forty-two strains belonged to the genera Sporobolomyces, Sporidiobolus, Aessosporon and Bullera and twenty-four strains to Rhodotorula, Rhodosporidium, Cryptococcus, unidentified ballistosporogenous yeasts, and Candida edax. Four Sporobolomyces salmonicolor strains, two Sporobolomyces holsaticus strains, two Sporobolomyces odorus strains, five Sporidiobolus salmonicolor strains, two Aessosporon salmonicolor strains, and one strain of Aessosporon dendrophilum produced similar electrophoretic patterns. Moreover, mating was observed between some of these strains. Sporobolomyces roseus and Sporobolomyces shibatanus differed from Sporobolomyces salmonicolor in their glucose-6-phosphate dehydrogenase (EC 1.1.1.49.), but the patterns of the other enzymes were similar; all three species differed clearly from Sporobolomyces singularis, Sporobolomyces gracilis, Sporobolomyces puniceus, and Sporobolomyces antarcticus. Sporobolomyces albo-rubescens showed a peculiar 6-phosphogluconate dehydrogenase (EC 1.1.1.41.) pattern and was similar to two Rhodotorula rubra strains in the pattern of their enzymes. Close relationships were also seen between Sporidiobolus ruinenii and Rhodotorula graminis, and between Bullera alba and a strain in Cryptococcus albidus var. albidus in the electrophoretic patterns of their enzymes. Four unidentified strains which had lost the ability to produce ballistospores had patterns similar to those of Bullera alba, Rhodotorula glutinis, Cryptococcus laurentii var. flavescens, and Cryptococcus macerans. Three colorless strains, putatively derived from a strain of Sporobolomyces roseus, showed the same electrophoretic patterns as the strains from which they originated.