A new species of rust fungus on the New Zealand endemic plant, Myosotidium, from the isolated Chatham Islands

Abstract

Pucciniastrum myosotidii sp. nov. is described from plants of the Chatham Island forget-me-not (Myosotidium hortensium), a host plant that has a conservation status of "nationally endangered". The rust has been found only on cultivated plants and not on wild plants. Although no teliospores were found, LSU and SSU sequence analysis showed that the new rust is closely related to some species of Pucciniastrum and Thekopsora forming a weakly supported clade together with P. boehmeriae, P. epilobii, P. circaeae, P. goeppertianum, P. guttatum, P. pustulatum, T. minima and Melampsorella symphyti. If this rust is endemic to Chatham Islands, then it must be accepted as a species of conservation value since the host plant is under threat from grazing animals and habitat loss.

Uromyces hawksworthii nom. nov. for Aecidium goyazense, on Phthirusa stelis (Loranthaceae) from the Brazilian Cerrado

“…Based on the BLASTn results, sequences were selected for the greatest similarity, and data from recent phylogenetic studies focused on Pucciniaceae ( Bruns et al 1992 , Maier et al 2003 , Chung et al 2004 , Aime 2006 , Matheny et al 2006 , Henricot et al 2007 , Maier et al 2007 , Yun et al 2010 , Dixon et al 2010 , Deadman et al . 2011 , Zuluaga et al 2011 , Busby et al 2012 , McTaggart 2014 , Padamse and McKenzie 2014 , Liu et al 2015 ). After selection, the sequences were downloaded in FASTA format and aligned by the multiple sequence alignment program MUSCLE® ( Edgar 2004 ), built in MEGA v. 6 software ( Tamura et al 2011 ).…”

Distribution of Pucciniastrum symphyti (Pucciniales) in Ukraine

“…Bubák but recent phylogenetic analysis has shown that it should be recombined in the genus Pucciniastrum G.H. Otth (Padamsee, McKenzie, 2014). Pucciniastrum symphyti is one of not so numerous rust species of predominantly European distribution with a few records from the Caucasus and Asia Minor.…”

Pucciniastrum minimum is the causal agent of rust on blueberries in New Zealand

“…corymbosum in Queensland and New South Wales in Australia, which prompted a re-examination of the New Zealand collections of rust fungi on blueberries. DNA from two recent collections (PDD 104137 and PDD 102321) was extracted and the 28S region (nuLSU) was amplified according to the protocol outlined in Padamsee and McKenzie (2014). The LSU sequence of PDD 104137 and P D D 1 0 2 3 2 1 ( G e n B a n k M K 3 5 3 4 1 9 a n d MK353420, respectively) had 99% identity (with 1 00 % q ue r y c ov er ag e) t o T he k op s o r a m i n i m a (HM439777) in a BLAST search.…”

“…The newly generated sequences were aligned with five other sequences of P. minimum (as T. minima) deposited in GenBank and 21 sequences from the Padamsee and McKenzie (2014) study, which demonstrated that Thekopsora minima is recovered in a clade that encompasses Pucciniastrum sensu stricto and as a result the appropriate name for this species should be Pucciniastrum miminum. The dataset was analysed in GARLI (Zwickl 2006) using the GTR + Γ + I model of evolution and 1000 maximum likelihood bootstrap support (MLBS) values were generated in PhyML 3.0 (Guindon and Gascuel 2003).…”