A New Species of Pseudopaludicola Miranda-Ribeiro, 1926 (Anura: Leptodactylidae: Leiuperinae) from an Amazonia-Cerrado Transitional Zone, State of Tocantins, Brazil

Reassessment of the taxonomic status of Pseudopaludicola parnaiba (Anura, Leptodactylidae, Leiuperinae), with the description of a new cryptic species from the Brazilian Cerrado

“…Fišer et al (2018) highlighted the importance of research efforts for fully integrating cryptic species into biodiversity science, thereby fostering a better understanding of the heterogeneous role of speciation in biodiversity pattern and process. Integrative taxonomy can notably improve the knowledge of frog diversity through species descriptions and delimitation, especially when dealing with morphologically cryptic species groups, recurrently reported for the frog genus Pseudopaludicola Miranda-Ribeiro, 1926 (e.g., Andrade et al 2016Andrade et al , 2018aAndrade et al , 2018bAndrade et al , 2019Pansonato et al 2016;Cardozo et al 2018).…”

“…canga) from Barreirinhas, MA, northern Brazil. Since then, the putative species has been treated as a species yet to be described (Veiga-Menoncello et al 2014;Pansonato et al 2014a;Andrade et al 2016Andrade et al , 2018aAndrade et al , 2018bAndrade et al , 2019Cardozo et al 2018).…”

“…Pseudopaludicola giarettai Carvalho, 2012 is a well-characterized species, based on acoustic and morphological data (Carvalho 2012;Carvalho et al 2015b). However, the phylogenetic position of the species remains to be tested (Andrade et al 2018a(Andrade et al , 2018b(Andrade et al , 2019. Carvalho et al (2015a) also characterized the call of a population of Pseudopaludicola from the municipality of Palmeiras de Goiás, GO, Brazil, with the same trilled advertisement call pattern as P. facureae.…”

A new tiny toad species ofAmazophrynella(Anura: Bufonidae) from east of the Guiana Shield in Amazonia, Brazil

“…Conditions for PCR amplification consisted of 1× buffer, dNTP at 0.2 mM, each primer at 0.2 µM, MgCl 2 at 2mM, 1U Taq polymerase and 2 µl of template DNA, in a total reaction volume of 25 µl. The PCR cycling program was run according to Lyra, Haddad & Azeredo-Espin (2017;COI) and Andrade et al (2019; 16s and 12s). We purified PCR products by Ethanol/ Sodium Acetate and bidirectionally sequenced them using an ABI 3130 Genetic Analyzer (Applied Biosystems).…”

“…Combining different approaches (e.g., morphological, bioacoustic, population genetics, ecology) have been successfully used to delimit new species in the Neotropics (e.g., Andrade et al, 2019;Mângia et al, 2018;Pereira et al, 2018), and revealed new taxa within many species complexes traditionally classified as a single nominal organism (Fouquet et al, 2012a;Fouquet et al, 2014;Gehara et al, 2013). Although the megadiverse Amazon Forest biome harbors many species that are widespread, many anurans are philopatric with poor dispersal abilities, which frequently results in strong genetic structure and allopatric distributions (Funk, Caminer & Ron, 2012;Pirani et al, 2020;Vacher et al, 2017).…”