A new species of Gieysztoria (Platyhelminthes, Rhabdocoela) from Argentina and a kinship analysis of South American species of the genus

Abstract

A new species of Gieysztoria (Platyhelminthes, Rhabdocoela) from Argentina, and a kinship analysis of South American species of the genus. -Zoologica Scripta , 32 , 449 -457. This study describes a new species of Gieysztoria found in lentic environments in Argentina. Gieysztoria falx sp. n. appears to be closely related to G. therapaina and G. intricata , and is differentiated principally by the presence of two strongly developed pincer-shaped spines of the cuticular stylet. A preliminary analysis of the phylogeny among the South American species of this genus is undertaken, and the monophyly verified. This study constitutes the first approach to interpreting these relationships.

Six freshwater microturbellarian species (Platyhelminthes) in permanent wetlands of the Coastal Plain of southern Brazil: new records, abundance, and distribution

“…The taxonomic works of Marcus (1945aMarcus ( , 1945bMarcus ( , 1946Marcus ( , 1948Marcus ( , 1949Marcus ( , 1950Marcus ( , 1952Marcus ( , 1954, Brusa et al (2003), Damborenea et al (2005), Larsson and Willems (2010), and the Turbellarian Taxonomic Database (Tyler et al 2006(Tyler et al -2016 were used for comparisons. Whenever possible, voucher specimens were fixed in absolute ethanol or Bouin (Romeis 1989) General morphology.…”

“…The specimens of turbellarians were treated by the oxygen reduction method by Braccini and Leal-Zanchet (2013), and after visual detection, they were transferred to a Petri-dish for examination (Gamo 1987a). In the present study, all sampled water was examined under the stereomicroscope (Brusa et al 2003), which favored the detection of small specimens (≤ 1 mm), even when their abundance was low, which is the case for many species of microturbellarians. However, this method requires more time, which reduces the number of samples for sorting after each field trip, because the identification of microturbellarians needs to be done in vivo.…”

“…All sampled water was gradually poured into a Petri-dish and examined under the stereomicroscope (Brusa et al 2003). Each turbellarian was counted and transferred to a histological slide and their external aspect was examined under the stereomicroscope.…”

Microturbellarians (Platyhelminthes and Acoelomorpha) in Brazil: invisible organisms?

“…Microturbellarians should be sorted alive under the stereomicroscope and analyzed under the microscope to study their morphology with identification purposes (Young, 2001;Brusa et al, 2003). Thus, some adjustments in the sampling procedures used for other invertebrates are needed, such as the collection of water for sorting the live microturbellarians at the laboratory.…”

“…The vials should be transported to the laboratory and carefully examined under a stereomicroscope (Braccini and Leal-Zanchet, 2013;Vara and Leal-Zanchet, 2013). For sorting, samples may be treated by the method of reduction of oxygen (Schockaert, 1996) or by examining all sampling water under the stereomicroscope (Brusa et al, 2003). Live turbellarians should be transferred by a pipette to a petri dish and be examined alive for observation of general shape, size and colour pattern.…”