A new specially designed needle significantly increases sample yield during fine needle aspiration of breast lesions

Wiksell, Hans; Ekstrand, Vilhelm; Wadström, C; Auer, Gert

doi:10.1016/j.ejmp.2008.01.009

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…This could in turn provide means to use less invasive clinical diagnostic methods where the amount of extracted sample is a limitation. 31 An interesting perspective would also be to develop new analytical tools which correlate data of multiple high resolution targets. Such algorithms could give better insights into more complex cellular mechanisms, pathways and interactions where many different proteins are involved, such as in system biology elucidations.…”

Section: Resultsmentioning

confidence: 99%

“…By increasing the number of targets, the specificity in this identification can be further increased and the amount of cells needed for a reliable diagnosis can be reduced. This could in turn provide means to use less invasive clinical diagnostic methods where the amount of extracted sample is a limitation . An interesting perspective would also be to develop new analytical tools which correlate data of multiple high resolution targets.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Multicolor Fluorescence Nanoscopy by Photobleaching: Concept, Verification, and Its Application To Resolve Selective Storage of Proteins in Platelets

Rönnlund

Perols

et al. 2014

ACS Nano

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Fluorescence nanoscopy provides means to discern the finer details of protein localization and interaction in cells by offering an order of magnitude higher resolution than conventional optical imaging techniques. However, these super resolution techniques put higher demands on the optical system and the fluorescent probes, making multicolor fluorescence nanoscopy a challenging task. Here we present a new and simple procedure, which exploits the photostability and excitation spectra of dyes to increase the number of simultaneous recordable targets in STED nanoscopy. We use this procedure to demonstrate four-color STED imaging of platelets with e40 nm resolution and low crosstalk. Platelets can selectively store, sequester, and release a multitude of different proteins, in a manner specific for different physiological and disease states. By applying multicolor nanoscopy to study platelets, we can achieve spatial mapping of the protein organization with a high resolution for multiple proteins at the same time and in the same cell. This provides a means to identify specific platelet activation states for diagnostic purposes and to understand the underlying protein storage and release mechanisms. We studied the organization of the pro-and antiangiogenic proteins VEGF and PF-4, together with fibrinogen and filamentous actin, and found distinct features in their respective protein localization. Further, colocalization analysis revealed only minor overlap between the proteins VEGF and PF-4 indicating that they have separate storage and release mechanisms, corresponding well with their opposite roles as pro-and antiangiogenic proteins, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%