A new SNP in the 3′UTR region of the bovine calpain small subunit (CAPNS1) gene

Juszczuk-Kubiak, Edyta; Flisikowski, Krzysztof; Wicińska, K.

doi:10.1007/s11033-009-9654-8

Cited by 14 publications

(7 citation statements)

References 11 publications

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“…These authors showed that nearly half of the animals under investigation were heterozygous at the analysed locus. Other studies support the high frequency of the CT genotype (0.58) within Holstein-Friesian cattle [6]. The same tests carried out for meat-producing breeds showed that the CT genotype was also most frequent-it was identified in about 56% of animals of the Limousine breed and 65% of animals of the Charolaise breed.…”

Section: Itemsupporting

confidence: 52%

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Polymorphisms of the CAPN1, CAST and MSTN Genes, and Their Association with Histological and Textural Parameters of Beef from Polish Holstein-Friesian Cattle

Węglarz¹,

Balakowska²,

Kułaj³

2019

JAST-A

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The purpose of this study was to analyse polymorphisms of the CAPN1, CAST and MSTN genes and their association with the microstructure of the Musculus longissimus thoracis (MLT) and textural parameters in bulls of the Holstein-Friesian breeds, black-and-white variety. The polymorphisms at the three loci: in position 6536 of the 3'UTR region of the CAPN1 gene, in position 230 of intron 5 in CAST gene, and in position 371 of the promoter region of the MSTN gene were analysed. Given the inconsequential genetic diversity at the analysed CAPN1 and MSTN loci in the animal sample, it was considered unreasonable to perform further statistical analyses aimed at determining associations between polymorphisms in these positions and meat characteristics. Based on an analysis of the CAST gene polymorphism, a significant association with certain histological and textural parameters was identified.

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Section: Itemsupporting

confidence: 52%

“…So far, however, few studies in this area concerned cattle reared in Poland, including the Holstein-Friesian breed. Moreover, not all of the studies have included an analysis of significant parameters which are of relevance to the quality of beef [6].…”

Section: Introduction mentioning

confidence: 99%

Polymorphisms of the CAPN1, CAST and MSTN Genes, and Their Association with Histological and Textural Parameters of Beef from Polish Holstein-Friesian Cattle

Węglarz¹,

Balakowska²,

Kułaj³

2019

JAST-A

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“…CAPN1S gene polymorphism identification of bovine muscles was performed with the assistance of the PCR-RFLP technique employing Mbo II endonuclease [10]. Primers with CAPN1S-F 5′-CCTCACTGTCTGTCCCTTCC-3′ and CAPN1S-R 5′-ACACAAATGTTGGGCTTGG-3′ sequences amplifying 332 bp were used in the experiment.…”

Section: Methodsmentioning

confidence: 99%

“…a regulatory subunit of m-calpain with the above-mentioned traits (meat and fat content in valuable cuts, tenderness, colour, taste, consistence, pH). Recently, polymorphism of a new SNP in the 30UTR region of the bovine μ-calpain small subunit ( CAPNS1 ) gene (exon 11—RFLP/ Mbo II) has been discovered [10]. …”

Section: Introductionmentioning

confidence: 99%

Impact of polymorphism of the regulatory subunit of the μ-calpain (CAPN1S) on the proteolysis process and meat tenderness of young cattle

et al. 2010

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The objective of this study was to estimate the impact of the polymorphism of μ-calpain (CAPN1S) gene on protein changes of the cattle muscle tissue and its tenderness during 10-day cold storage. The analysis was performed on the longest dorsal and lumbar muscles collected from 76 bulls 6 to 12 months of age. Polymorphism identification of the above-mentioned gene was conducted using the PCR-RFLP technique. Its effect on the course of the proteolysis process was assessed by monitoring changes in proportions of tissue proteins during 10-day process of meat ageing. Special attention was focused on changes in native titin (T1) share and products of its degradation (proteins of molecular weight (m.w.) of 2400 and 200 kDa), α-actinin and protein of 37 kDa as well as myosin heavy chains (MHC). In the case of the last proteins, their polymorphism was evaluated as well. Meat tenderness was estimated measuring the value of shear force and sensorially. The highest tenderness was ascertained for the heterozygote. Its improvement was associated with a significant decrease in proportions of proteins of molecular weight of approximately 37 kDa accompanied by an increase of those with 200 kDa molecular weight. Muscles derived from cattle of CT genotype were characterised by the highest proportions of type 2a MHC isoform. Value differences between proportions determined for the heterozygote and CC and TT homozygotes of the CAPN1S gene were statistically significant. Therefore, it can be presumed that the process of meat tenderisation was especially connected with MHC polymorphism.

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“…Particularly, 3 0 UTRs are rich in cis-acting regulatory elements [24]. Juszczuk-Kubiak et al [25] showed that the SNP in the 3 0 UTR of the bovine calpain small subunit gene was associated with meat quality. The mutations g.14248 T > C and g.14391 C> T may affect gene regulation and selective splicing regulation, although g.14248 T > C will not affect the amino acid sequence [26], and subsequently affect the serum concentration and activity of MASP2.…”

Section: Relationship Of Genotypes and Haplotypes Of Masp2 With Milk mentioning

confidence: 99%

Polymorphisms of MASP2 gene and its relationship with mastitis and milk production in Chinese Holstein cattle

Zhang

Yan

Zhang

et al. 2019

Biotechnology & Biotechnological Equipment

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Mastitis is a deleterious disease of dairy cattle, and its incidence is closely related with the innate immune response, including the complement system. Mannose-binding lectin-associated serine protease 2 (MASP2) is a functional central enzyme in the complement system. Here, we aimed to study single nucleotide polymorphisms (SNPs) of MASP2 and its relationship with mastitis and milk production in Chinese Holstein cattle. We found three novel SNPs, namely g.14047A > C, g.14248T > C and g.14391C > T, in MASP2. The SNP g.14047 A > C (GAA (Glu)>GAC (Asp)) at position 608aa of MASP2 was correlated with the CH50 level and the protein percentage. Meanwhile, the SNP g.14248 T > C (ATT (Ile) > ATC (Ile)) at position 675aa was also correlated with the CH50 value and the milk production. The haplotype combinations H2H2 and H2H4 showed a significant relationship with the CH50, SCS and the milk production abilities, respectively. Based on the SNPs identified in this gene, several genotypes and haplotypes, respectively, were found in Chinese Holstein cattle. The genotypes AC (g.14047 A > C) and TT (g.14248 T > C) and the haplotype combinations H2H2 and H2H4 may be used as molecular markers for breeding mastitis-resistant dairy cattle. The haplotype combination H1H2 should be gradually phased out in breeding programmes.

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A new SNP in the 3′UTR region of the bovine calpain small subunit (CAPNS1) gene

Cited by 14 publications

References 11 publications

Polymorphisms of the CAPN1, CAST and MSTN Genes, and Their Association with Histological and Textural Parameters of Beef from Polish Holstein-Friesian Cattle

Polymorphisms of the CAPN1, CAST and MSTN Genes, and Their Association with Histological and Textural Parameters of Beef from Polish Holstein-Friesian Cattle

Impact of polymorphism of the regulatory subunit of the μ-calpain (CAPN1S) on the proteolysis process and meat tenderness of young cattle

Polymorphisms of MASP2 gene and its relationship with mastitis and milk production in Chinese Holstein cattle

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