A New Small RNA Virus Persistently Infecting an Established Cell Line ofGalleria mellonella,Induced by a Heterologous Infection

Léry, Xavier; Fédière, Gilles; Taha, Ahmed; Salah, M.; Giannotti, J.

doi:10.1006/jipa.1996.4617

Cited by 11 publications

(8 citation statements)

References 19 publications

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“…However, TNCL virus was detected in AcMNPV-infected High Five cells (passage 98) used as a positive control. Persistent viral infections have been observed occasionally in insect cell lines [22,23] and nodaviruses are thought to occasionally infect wild marine invertebrates either latently or persistently [24]. A betanodavirus was also reported to be latently infecting a cell line derived from the brain tissue of the barramundi fish, Lates calcarifer [25].…”

Section: Discussionmentioning

confidence: 99%

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

2010

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BackgroundThe insect cell line is a critical component in the production of recombinant proteins in the baculovirus expression system and new cell lines hold the promise of increasing both quantity and quality of protein production.ResultsSeventy cell lines were established by single-cell cloning from a primary culture of cells derived from eggs of the black witch moth (Ascalapha odorata; Lepidoptera, Noctuidae). Among 8 rapidly growing lines, cell line 38 (Ao38) was selected for further analysis, based on susceptibility to AcMNPV infection and production of secreted alkaline phosphatase (SEAP) from a baculovirus expression vector. In comparisons with low-passage High Five (BTI-Tn-5B1-4) cells, infected Ao38 cells produced β-galactosidase and SEAP at levels higher (153% and 150%, respectively) than those measured from High Five cells. Analysis of N-glycans of SEAP produced in Ao38 cells revealed two N-glycosylation sites and glycosylation patterns similar to those reported for High Five and Sf9 cells. Glycopeptide isoforms consisted of pauci- or oligomannose, with and without fucose on N-acetylglucosamine(s) linked to asparagine residues. Estimates of Ao38 cell volume suggest that Ao38 cells are approximately 2.5× larger than Sf9 cells but only approximately 74% of the size of High Five cells. Ao38 cells were highly susceptible to AcMNPV infection, similar to infectivity of Sf9 cells. Production of infectious AcMNPV budded virions from Ao38 cells peaked at approximately 4.5 × 107 IU/ml, exceeding that from High Five cells while lower than that from Sf9 cells. Ao38 cells grew rapidly in stationary culture with a population doubling time of 20.2 hr, and Ao38 cells were readily adapted to serum-free medium (Sf-900III) and to a suspension culture system. Analysis of Ao38 and a parental Ascalapha odorata cell line indicated that these lines were free of the alphanodavirus that was recently identified as an adventitious agent in High Five cell lines.ConclusionsAo38 cells represent a highly productive new insect cell line that will be useful for heterologous protein expression and other applications in biotechnology.

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Section: Discussionmentioning

confidence: 99%

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

2010

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“…Two other persistent viruses in lepidopteran cell lines are Galleria mellonella cell line virus (GmCLV) and Perina nuda picorna-like virus. GmCLV replication was induced by infection of Gm120 cells with an unrelated picorna-like virus as well as Autographa californica multiple nucleopolyhedrovirus (AcMNPV; Lery et al 1997). Upon the induction of GmCLV, neither the picorna-like virus nor AcMNPV replicated.…”

Section: Insect Cell Lines Are Tools In Virologymentioning

confidence: 99%

Insect cell culture and applications to research and pest management

Smagghe

Goodman

Stanley

2009

In Vitro Cell.Dev.Biol.-Animal

104

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Building on earlier research, insect cell culture began with the successful establishment of one cell line from pupal ovarian tissue. The field has grown to the extent that now over 500 insect cell lines have been established from many insect species representing numerous insect orders and from several different tissue sources. These cell lines are used as research tools in virology, in studies of signaling mechanisms to study insect immunity, hemocyte migration, and to test hypotheses about gene expression, and in screening programs designed to discover new insecticide chemistries. Virology research is revealing fundamentally new information on virus/host cell interactions. Studies in gene expression are uncovering signal transduction pathways that are new to insect science. Research is leading to the development of high-speed screening technologies that are essential in the search for new insect pest management tools. A few insect cell lines are, in routine industrial processes, designed to produce proteins of biomedical significance. Both primary cell cultures and established lines are used in basic biological studies to reveal how insect cells work. This review is designed to briefly cover the history of insect cell culture, recount some recent advances in the field, and offer a vision of the future of insect cell culture.

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“…The in vitro propagation of two insect picornaviruses, cricket paralysis virus (CrPV) and Drosophila C virus (DCV), in D. melanogaster cells was first successfully achieved in the early 1980s (Moore and Pullin, 1982;Moore et al, 1981). Recently, a persistent infection of GmcIV (Galleria mellonella cell line virus, Picornaviridae) in a G. mellonella cell line was also reported, and in the presence of MSBV, a maize stem borer picornalike virus isolated from Seasamia cretica, observable cytopathic effects have also been induced (Lery et al, 1997). In the present paper, we report for the first time the successful propagation of PnPV in its homogenous cell line, NTU-PN-HH.…”

Section: Discussionmentioning

confidence: 60%

A New Picorna-like Virus, PnPV, Isolated from Ficus Transparent Wing Moth, Perina nuda (Fabricius)

Wang

1999

Journal of Invertebrate Pathology

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A New Small RNA Virus Persistently Infecting an Established Cell Line ofGalleria mellonella,Induced by a Heterologous Infection

Cited by 11 publications

References 19 publications

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

Ao38, a new cell line from eggs of the black witch moth, Ascalapha odorata (Lepidoptera: Noctuidae), is permissive for AcMNPV infection and produces high levels of recombinant proteins

Insect cell culture and applications to research and pest management

A New Picorna-like Virus, PnPV, Isolated from Ficus Transparent Wing Moth, Perina nuda (Fabricius)

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