A new small-angle neutron scattering spectrometer at China Mianyang research reactor

Mei, Peng; Sun, Laixi; Chen, Liang; Sun, Guangai; Chen, Bin; Xie, Changsheng; Qingzhong, Xia; Yan, Guanyun; Tian, Qiang; Huang, Congcong; Pang, Beibei; Zhang, Ying; Wang, Yun; Liu, Yao-Guang; Wu, Kang; Gong, Jian

doi:10.1016/j.nima.2015.11.141

Cited by 70 publications

(41 citation statements)

References 17 publications

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“…The current decade has witnessed an increase in the number and the geographical spread of new SANS instruments (Peng et al, 2016;Mü hlbauer et al, 2016;Dewhurst et al, 2016;Wood et al, 2015;Zhang et al, 2014;Vogtt et al, 2014;Heller et al, 2014;Rehm et al, 2013;Han et al, 2013;Liu et al, 2011;Zhao et al, 2010) and in upgrades of existing ones (Stone et al, 2016;Radulescu et al, 2015;Feoktystov et al, 2015;Wignall et al, 2012;Iwase et al, 2011;Goerigk & Varga, 2011). Most of these instruments are designed to study various sample types (both hard and soft matter) from diverse scientific fields, including biology, chemistry, physics and material sciences.…”

Section: Sans Beamlines and Sample Environmentsmentioning

confidence: 99%

Biological small-angle neutron scattering: recent results and development

Mahieu

Gabel

2018

Acta Cryst Sect D Struct Biol

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Small-angle neutron scattering (SANS) has increasingly been used by the structural biology community in recent years to obtain low-resolution information on solubilized biomacromolecular complexes in solution. In combination with deuterium labelling and solvent-contrast variation (HO/DO exchange), SANS provides unique information on individual components in large heterogeneous complexes that is perfectly complementary to the structural restraints provided by crystallography, nuclear magnetic resonance and electron microscopy. Typical systems studied include multi-protein or protein-DNA/RNA complexes and solubilized membrane proteins. The internal features of these systems are less accessible to the more broadly used small-angle X-ray scattering (SAXS) technique owing to a limited range of intra-complex and solvent electron-density variation. Here, the progress and developments of biological applications of SANS in the past decade are reviewed. The review covers scientific results from selected biological systems, including protein-protein complexes, protein-RNA/DNA complexes and membrane proteins. Moreover, an overview of recent developments in instruments, sample environment, deuterium labelling and software is presented. Finally, the perspectives for biological SANS in the context of integrated structural biology approaches are discussed.

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Section: Sans Beamlines and Sample Environmentsmentioning

confidence: 99%

Biological small-angle neutron scattering: recent results and development

Mahieu

Gabel

2018

Acta Cryst Sect D Struct Biol

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“…Neutron wavelength of 5 and 10Å, and sample-detector distances 1.3 and 5.5 m were used; the measurements were carried out at room temperature. Preliminary SANS experiments were conducted on Suanni SANS instrument installed at CMRR, Mianyang, China [21]. X-ray powder diffraction (XRD) measurements were performed on Panalytical X'Pert Pro MPD diffractometer equipped with Cu-anode (Cu Kα; λ=1.54181Å) and X'Celerator detector.…”

Section: Characterizationmentioning

confidence: 99%

Mesoporous silica obtained with methyltriethoxysilane as co-precursor in alkaline medium

Putz

Wang

Len

et al. 2017

Applied Surface Science

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Mesoporous silica particles have been synthesized by sol-gel method from tetraethoxysilane (tetraethylorthosilicate, TEOS) and methyltriethoxysilane (MTES), in ethanol and water mixture, at different ratios of the of the silica precursors. Ammonia was used as catalyst at room temperature and hexadecyltrimethylammonium bromide (cetyltrimethylammonium bromide, CTAB) as the structure directing agent. Nitrogen sorption, X-ray diffraction and small-angle neutron scattering gave information on the evolution of the gel structure and pore morphologies in the function of MTES/TEOS molar ratio. Thermogravimetric and differential thermal analysis showed that with addition of MTES the exothermic peak indicating the oxidation of the low molecular weight organic fragments shift to higher temperature. A room-temperature, one-pot synthesis of MCM-41 type materials is presented, in which the variation of the MTES concentration allows to change the hydrophobicity, preserving the specific properties materials, like the ordered pore structure, large specific surface area and high porosity, making them suitable for selective uptake of guest species in drug loading applications. Specifically, the obtained materials had cylindrical pores, specific surface areas up to 1101 m 2 /g and total pore volumes up to 0.473 cm 3 /g. The obtained mesoporous materials are susceptible for further functionalization to improve their selective uptake of guest species in drug delivery applications.

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“…The small-angle neutron scattering (SANS) spectrometer Suanni (Peng et al, 2016), which has recently been installed and operated at the liquid hydrogen cold neutron source of the 20 MW China Mianyang Research Reactor (CMRR), is similar to several conventional pinhole SANS (PSANS) instruments operated at major neutron research facilities across the world. SANS is a valuable method for investigating structures on typical length scales ranging from 1 to 500 nm in polymeric (Mortensen, 1996), biological (Liu et al, 2010;Guo et al, 1990), magnetic (Lebech et al, 1989;Wollan & Koehler, 1955) and complex-fluid (Smith et al, 2014;Yurekli et al, 2004) materials.…”

Section: Introductionmentioning

confidence: 99%

“…In this work, we aim to improve the performance of the PSANS spectrometer Suanni at CMRR (Peng et al, 2016). Ultra-thin biconcave MgF 2 lenses with a central thickness down to 0.2 mm are introduced.…”

Section: Introductionmentioning

confidence: 99%

“…Suanni is mainly constituted of a multi-blade mechanical velocity selector, two sets of collimation systems (single-beam and multi-beam modes, four source apertures), a sample stage and a flight tube containing a two-dimensional 3 He position-sensitive detector. Details of the features of the original Suanni PSANS spectrometer have been reported elsewhere (Peng et al, 2016). In the following section, we will briefly explain the design of the focusing lenses, the basic theories of the FSANS setup, the calculation, and the experimental measurements with and without focusing lenses.…”

Section: Introductionmentioning

confidence: 99%

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Small-angle neutron scattering spectrometer Suanni equipped with ultra-thin biconcave focusing lenses

et al. 2016

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The small-angle neutron scattering (SANS) spectrometer Suanni at the liquid hydrogen cold neutron source of the 20 MW China Mianyang Research Reactor has recently been upgraded. Ultra-thin biconcave MgF 2 lenses with a central thickness down to 0.2 mm have been installed between the collimator chamber and the sample stage. The lenses are able to improve the flux without too excessive an increase in the neutron beam size on the detector. A smaller minimum Q (Q min ) can be obtained by decreasing the beam size without changing the total length of the spectrometer. By testing the central beam profiles under different neutron wavelengths ($0.56-1 nm) with both traditional pinhole SANS (PSANS) and focusing SANS (FSANS) geometries, the gain factor thanks to the neutron lenses is about one order of magnitude. Given the loss of intensity due to the absorption of neutrons by the lenses, the benefits of the focusing can only be realized if it is possible to increase the aperture size. With an identical source aperture, FSANS can minimize the nominal Q min from 7.20 Â 10 À3 nm À1 (for PSANS) to 5.55 Â 10 À3 nm À1 at a neutron wavelength of 1 nm. The practical benefit provided by the lenses is verified with a solution of poly(methyl methacrylate) nanospheres, which yields a scattering intensity one order of magnitude higher and a better resolution with the FSANS geometry than with that of PSANS.

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A new small-angle neutron scattering spectrometer at China Mianyang research reactor

Cited by 70 publications

References 17 publications

Biological small-angle neutron scattering: recent results and development

Biological small-angle neutron scattering: recent results and development

Mesoporous silica obtained with methyltriethoxysilane as co-precursor in alkaline medium

Small-angle neutron scattering spectrometer Suanni equipped with ultra-thin biconcave focusing lenses

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