A new single-chain variable fragment (scFv) antibody provides sensitive and specific detection of citrus tristeza virus

Raeisi, Hamideh; Safarnejad, Mohammad Reza; Sadeghkhani, Farideh

doi:10.1016/j.jviromet.2021.114412

Cited by 8 publications

(4 citation statements)

References 44 publications

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“…In addition, the ability of antibodies to block or outcompete specific binding interactions Frontiers in Nanotechnology frontiersin.org has led to their use in neutralising viruses and toxins (Cheedarla and Hanna, 2019). As the classical hybridoma technology developed with murine B cells (Köhler and Milstein, 1975) has proven difficult to adapt to the generation of human monoclonal antibodies, however, recombinant approaches have been widely developed to create human antibodies, or derived antibody fragments (Wronska et al, 2016), which can be used in vivo in applications such as drug delivery and neutralisation, and in vitro methodologies like immunodiagnosis (Raeisi et al, 2022;Wang et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

Antoine

Mohammadi

McDermott

et al. 2022

Front. Nanotechnol.

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COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2. From its initial appearance in Wuhan, China in 2019, it developed rapidly into a global pandemic. In addition to vaccines, therapeutic antibodies play an important role in immediately treating susceptible individuals to lessen severity of the disease. In this study, phage display technology was utilised to isolate human scFv antibody fragments that bind the receptor-binding domain (RBD) of SARS-CoV-2 Wuhan-Hu-1 spike protein. Of eight RBD-binding scFvs isolated, two inhibited interaction of RBD with ACE2 protein on VeroE6 cells. Both scFvs also exhibited binding to SARS-CoV-2 Delta variant spike protein but not to Omicron variant spike protein in a Raman spectroscopy immunotest. The study demonstrates the potential of recombinant antibody approaches to rapidly isolate antibody moieties with virus neutralisation potential.

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Section: Discussionmentioning

confidence: 99%

Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

Antoine

Mohammadi

McDermott

et al. 2022

Front. Nanotechnol.

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“…Additionally, rAbs can be used as an alternative attractive strategy to mAbs and pAbs. Successful application of scFv antibodies in various ELISA platforms is reported in many studies; for instance, the development of ELISA based on phage-displayed scFv antibodies was reported for detecting B. melitensis ( Hayhurst et al, 2003 ), IFN-γ antigen ( Yang et al, 2018 ), cowpea chlorotic dwarf virus ( Raeisi et al, 2020 ), citrus tristeza virus ( Raeisi et al, 2022b ; Chen et al, 2015b ), the protein components of type three secretion system of Xanthomons citri ( Raeisi et al, 2019 ), B. thuringiensis ( Dong et al, 2018 ) and so on. Moreover, several publications have reported the development of diagnostic VHH-based ELISA assays for rapid diagnosis of specific targets.…”

Section: Development Of Rapid Detection Methods Based On Recombinant ...mentioning

confidence: 99%

“…In this regard, antibodies are exploited due to their high affinity and specific binding activity toward target molecules, which highlights their effectiveness as theranostics tools. In recent decades, recombinant antibody (rAb) technologies have widely received much attention as diagnostic tools ( Xu et al, 2017 ; Shali et al, 2018 ; Fouladi et al, 2019 ; Raeisi et al, 2019 ; Alibeiki et al, 2020 ; Raeisi et al, 2020 , 2022b ; Berry et al, 2022 ). The rAb technologies that involve the construction of large libraries of different antibody fragments, such as fragment antigen-binding (Fab), single-chain fragment variable (scFv), minibodies, and nanobodies, can screen antibodies in vitro based on their binding properties, thus help develop more cost-effective antibodies with high sensitivity and specificity ( Bockstaele et al, 2000 ; Angela Chiew Wen et al, 2016 ; Raeisi et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid-format recombinant antibody-based methods for the diagnosis of Clostridioides difficile infection: Recent advances and perspectives

et al. 2022

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Clostridioides difficile, the most common cause of nosocomial diarrhea, has been continuously reported as a worldwide problem in healthcare settings. Additionally, the emergence of hypervirulent strains of C. difficile has always been a critical concern and led to continuous efforts to develop more accurate diagnostic methods for detection of this recalcitrant pathogen. Currently, the diagnosis of C. difficile infection (CDI) is based on clinical manifestations and laboratory tests for detecting the bacterium and/or its toxins, which exhibit varied sensitivity and specificity. In this regard, development of rapid diagnostic techniques based on antibodies has demonstrated promising results in both research and clinical environments. Recently, application of recombinant antibody (rAb) technologies like phage display has provided a faster and more cost-effective approach for antibody production. The application of rAbs for developing ultrasensitive diagnostic tools ranging from immunoassays to immunosensors, has allowed the researchers to introduce new platforms with high sensitivity and specificity. Additionally, DNA encoding antibodies are directly accessible in these approaches, which enables the application of antibody engineering to increase their sensitivity and specificity. Here, we review the latest studies about the antibody-based ultrasensitive diagnostic platforms for detection of C. difficile bacteria, with an emphasis on rAb technologies.

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“…We define laboratory based molecular tests as the current standard diagnostic practice involving protocols and instrumentation not easily deployed in field testing. Such molecular tests provide accurate and early identification of plant pathogens in centralized laboratories using genetic and serological analyses, such as reverse-transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunoassay (ELISA) and gel-blots analysis. − These molecular diagnostic techniques can identify specific pathogens and, in the case of RT-PCR, can diagnose infections early in presymptomatic plants, due to superior sensitivity . However, these protocols and instruments cannot be used directly in the field and are often costly.…”

mentioning

confidence: 99%

Direct Detection of Tobacco Mosaic Virus in Infected Plants with SERS-Sensing Hydrogels

Kissell,

Liu,

Sheokand

et al. 2024

ACS Sens.

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The impact of plant pathogens on global crop yields is a major societal concern. The current agricultural diagnostic paradigm involves either visual inspection (inaccurate) or laboratory molecular tests (burdensome). While field-ready diagnostic methods have advanced in recent years, issues remain with detection of presymptomatic infections, multiplexed analysis, and requirement for in-field sample processing. To overcome these issues, we developed surface-enhanced Raman scattering (SERS)sensing hydrogels that detect pathogens through simple contact with a leaf. In this work, we developed a novel reagentless SERS sensor for the detection of tobacco mosaic virus (TMV) and embedded it in an optimized hydrogel material to produce sensing hydrogels. To test the diagnostic application of our sensing hydrogels, we demonstrate their use to detect TMV infection in tobacco plants. This technology has the potential to shift the current agricultural diagnostic paradigm by offering a field-deployable tool for presymptomatic and multiplexed molecular identification of pathogens.

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A new single-chain variable fragment (scFv) antibody provides sensitive and specific detection of citrus tristeza virus

Cited by 8 publications

References 44 publications

Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

Rapid-format recombinant antibody-based methods for the diagnosis of Clostridioides difficile infection: Recent advances and perspectives

Direct Detection of Tobacco Mosaic Virus in Infected Plants with SERS-Sensing Hydrogels

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