A new simple and rapid electrochemical method for the determination of hydroxyl radical generated by Fenton reaction and its application

Abstract

A simple and rapid electrochemical method to detect the hydroxyl radical is described. This method employed the reaction between.OH and dimethyl sulfoxide (DMSO) to generate quantitatively formaldehyde, which then reacted with hydrazine hydrochloride at pH 6.2. The product showed a second-order derivative cathodic wave with the peak potential of -1.08 V( vsSCE) by single sweep oscillopolarography. The electrochemical behavior of the product was investigated by single sweep oscillopolarography and cyclic voltammetry. The experimental conditions for the measurement were optimized and the scavenging activity of some flavonoids on hydroxyl radicals was studied.

Review of Methods to Determine Antioxidant Capacities

“…Though this method is handy and sensitive, the multiple reaction products make it complicated to quantitative detection of hydroxyl radical. Hantzsch reaction method and TBA method are simple and do not need expensive instruments; however, both of the reactions need high temperature (60-100°C) and long reaction time (30-60 min; Zou et al 2002). Moreover, the organic mobile phase of the HPLC assay, in which Á HO is generally detected by determining the dihydroxylated benzoic acid products derived from salicylic acid, will possibly cause environmental pollution and harm to health (Cheng et al 2003).…”

Selective Detection of Hydroxyl Radical Scavenging Capacity Based on Electrogenerated Chemiluminescence Detection Using Tris(2,2'-bipyridine)ruthenium(III) by Flow Injection Analysis

“…However, addition of extra H 2 O 2 may accelerate the production of · OH. 14,16) The results showed that the background emission increased with H 2 O 2 concentration in the range of 1-3 mM and remained constant thereafter. Therefore, 3 mM concentration of H 2 O 2 should be selected.…”

“…Commonly used methods for detecting · OH include electron spin resonance (ESR) [7][8][9] ; chemiluminescence 10,11) ; and high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection, 12) electrochemical detection (ED), [13][14][15] and fluorometric detection. 16,17) The ESR method has been widely accepted to measure · OH scavenging capacity, but it requires expensive instrumentation and cannot be readily used to obtain quantitative estimates of · OH adducts because the · OH spin is unstable and may react with other products.…”

Mapping of RNA–protein interactions

“…Originally, hydroxyl radical cleavage mapping had been used for DNA and macromolecule interactions determined by DNase I protected footprinting assay. Later chemical cleavage improvements to this technique were proposed by researchers [66][67][68][69][70]. Among these developments, Tullius and Dombroski [69,70] initiated a wonderful and still commonplace technique in which hydroxyl radicals are produced by reduction of H 2 O 2 by an EDTA-iron complex, cleaving DNA at positions accessible to the solvent without cleaving at the DNA-protein interactive sites.…”