A New Set of Nuclear Microsatellites for an Ecologically and Economically Important Conifer: the European Black Pine (Pinus nigra Arn.)

Giovannelli, Guia; Roig, Anne; Spanu, Ilaria; Vendramin, Giovanni G.; Fady, Bruno

doi:10.1007/s11105-017-1029-z

Cited by 10 publications

(18 citation statements)

References 43 publications

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“…Four paternally inherited cpSSRs, previously identified as polymorphic in black pine, were selected for this study (Appendix S1, Table S1.1 in the Supporting Information; Vendramin et al 1996). The 14 biparentally inherited nSSRs used were those characterized for black pine by Giovannelli et al (2017 ; Table S1.2 in Appendix S1). The 14 putatively adaptive nuclear genes selected for this study were those that could be transferred from P. taeda (Mosca et al 2012) in a preliminary test (Table S1.3 in Appendix S1).…”

Section: Genotyping and Sequencingmentioning

confidence: 99%

Recent, Late Pleistocene fragmentation shaped the phylogeographic structure of the European black pine (Pinus nigra Arnold)

Scotti‐Saintagne

Giovannelli

Roig

et al. 2019

Tree Genetics & Genomes

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Section: Genotyping and Sequencingmentioning

confidence: 99%

Recent, Late Pleistocene fragmentation shaped the phylogeographic structure of the European black pine (Pinus nigra Arnold)

Scotti‐Saintagne

Giovannelli

Roig

et al. 2019

Tree Genetics & Genomes

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“…The Portuguese P. nigra populations were planted 50-to 90-years ago (Dias et al 2018) with plant material of unknown origin and infraspecific taxonomy. Different molecular markers, including the ISSRs, have been used to characterize P. nigra populations (Liber et al 2003;Naydenov et al 2006;Afzal-Rafii and Dodd 2007;Rubio Moraga et al 2012;Lucas Borja et al 2013;Candel Pérez 2014;Š arac et al 2015;Bonavita et al 2016;Giovannelli et al 2017). The SCoT markers have been successfully used alone or combined with other markers for genetic diversity and fingerprinting in various species (Collard and Mackill 2009;Cabo et al 2014;Alikhani et al 2014;Zhang et al 2015), but have never been used in P. nigra.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Portuguese allochthonous populations of Pinus nigra using ISSRs and SCoTs and extrapolation of their infraspecific taxonomy

Dias

Lemos

Pavia

et al. 2019

Physiol Mol Biol Plants

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The Pinus nigra distribution in Portugal is restricted to six allochthonous populations with unknown origin and infraspecific taxonomy. This work intends to evaluate their genetic diversity, structure and relationships, and to infer about their infraspecific taxonomy by comparing molecular patterns produced by inter-simple sequence repeat and Start Codon Targeted markers among Portuguese and foreign samples with known taxonomy and provenance. 127 Portuguese P. nigra individuals were clustered per population. The genetic differentiation was higher within rather than among populations. The pooled molecular data indicated high genetic proximity among the Portuguese and foreign samples of subspecies laricio. However, the separate analysis per marker system demonstrated that two varieties of subspecies laricio (corsicana and calabrica) may have been used in the plantations of the Portuguese P. nigra stands performed in the last century. The genetic characterization and extrapolation of the intraspecific taxonomy of these populations provide useful information for forest management, afforestation and germplasm use.

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“…Besides, the information about intra-and inter-population, genetic diversity is also relevant for the maintenance of the stability of forest ecosystems, the adaptive potential to biotic and abiotic stresses (Muller-Starck et al 1992;Çengel et al 2012), and to assign high priority populations that deserve to be preserved and managed (Bonavita et al 2016). Knowledge of genetic diversity can give insights about the adaptive potential and/or the evolutionary history of a species, being particularly crucial in trees with a long life cycle (Hamrick 2004;Giovannelli et al 2017). The gathering of genetic diversity and structure information of forestry populations is helpful for the development of suited and effective strategies of conservation, management, germplasm use and genetic breeding (Frankham et al 2002;Hamrick 2004;Lučić et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Portuguese Pinus nigra J.F. Arnold populations: genetic diversity, structure and relationships inferred by SSR markers

Dias

Giovannelli

Fady

et al. 2020

Annals of Forest Science

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& Key message Pinus nigra J.F. Arnold has an ecological and economic interest in Europe, but many of the planted populations have an unknown origin and infraspecific taxonomy. Six Portuguese P. nigra populations characterised with microsatellites revealed high intra-population diversity structured into two clusters with low differentiation that might suggest two provenances or infraspecific taxa. Despite compared with foreign samples from different subspecies, we were not able to infer about the origin or infraspecific taxonomy of the Portuguese populations based on the pooled microsatellite data. & Context Many of the European Pinus nigra J.F. Arnold forests were planted with the material of unknown origin. Efforts have been made to determine their provenances and infraspecific taxonomy regarding their relevance for defining strategies of genetic resources conservation, germplasm use, forest management and genetic improvement. The Portuguese P. nigra populations are allochthonous, and their provenance and infraspecific taxonomy are unknown. & Aims With this work, we intended to characterise the Portuguese P. nigra populations regarding its genetic diversity, structure and relationships using simple sequence repeat (SSR) markers and/or to infer about its origin and infraspecific taxonomy by comparing the SSR patterns of foreign P. nigra samples of known taxonomic classification. & Methods A total of 224 Portuguese P. nigra individuals from six populations were characterised using 14 SSR markers developed specifically to different Pinus sp., including P. nigra, by other authors. & Results Thirteen SSR loci were selected and showed 100% of polymorphism among individuals. The genetic diversity was higher within (95%) rather than among (5%) populations. The Portuguese individuals were structured into two main clusters (K = 2) with low genetic differentiation (F ST = 0.04), and the foreign samples were genetically distant from the Portuguese individuals. & Conclusion The six Portuguese P. nigra populations revealed high genetic diversity and seemed to be structured into two main clusters with low differentiation suggesting two provenances or infraspecific taxa. Nonetheless, the comparative analyses with foreign samples did not allow a clear inference about its origin and/or infraspecific taxonomy. Additional foreign samples and/or molecular markers will be tested to pursue these goals.

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A New Set of Nuclear Microsatellites for an Ecologically and Economically Important Conifer: the European Black Pine (Pinus nigra Arn.)

Cited by 10 publications

References 43 publications

Recent, Late Pleistocene fragmentation shaped the phylogeographic structure of the European black pine (Pinus nigra Arnold)

Recent, Late Pleistocene fragmentation shaped the phylogeographic structure of the European black pine (Pinus nigra Arnold)

Genetic characterization of Portuguese allochthonous populations of Pinus nigra using ISSRs and SCoTs and extrapolation of their infraspecific taxonomy

Portuguese Pinus nigra J.F. Arnold populations: genetic diversity, structure and relationships inferred by SSR markers

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