A new set of microsatellite markers for the genetic characterization of Ceratocystis fimbriata, an economically important plant pathogen

Rizatto, Simone; Batista, Carlos Eduardo de Araújo; Bajay, Miklos Maximiliano; Sigrist, Mário Sérgio; Ito, Margarida Fumiko; Monteiro, Magali; Cavallari, Marcelo Mattos; Pinheiro, José Baldin; Zucchi, Maria Imaculada

doi:10.1007/s12686-009-9144-2

Cited by 2 publications

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“…However, when polymorphic loci are available, they can be very useful for genome mapping, and genetic diversity and population genetic studies [ 60 ]. Accordingly, SSRs have been used for the study of the genetic diversity of various plant pathogenic fungi including Ascochyta rabiei [ 61 ], Ceratocystis fimbriata [ 62 ], Macrophomina phaseolina [ 63 ], Puccinia graminis and P . triticina [ 6 , 64 , 65 ], Sclerotinia subarctica and S .…”

Section: Introductionmentioning

confidence: 99%

Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia

et al. 2016

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Twenty three polymorphic microsatellite markers were developed for citrus plant pathogenic fungus, Colletotrichum gloeosporioides, and were used to analyze genetic diversity and population structure of 163 isolates from four different geographical regions of Ethiopia. These loci produced a total of 118 alleles with an average of 5.13 alleles per microsatellite marker. The polymorphic information content values ranged from 0.104 to 0.597 with an average of 0.371. The average observed heterozygosity across all loci varied from 0.046 to 0.058. The gene diversity among the loci ranged from 0.106 to 0.664. Unweighted Neighbor-joining and population structure analysis grouped these 163 isolates into three major groups. The clusters were not according to the geographic origin of the isolates. Analysis of molecular variance showed 85% of the total variation within populations and only 5% among populations. There was low genetic differentiation in the total populations (FST = 0.049) as evidenced by high level of gene flow estimate (Nm = 4.8 per generation) among populations. The results show that Ethiopian C. gloeosporioides populations are generally characterized by a low level of genetic diversity. The newly developed microsatellite markers were useful in analyzing the genetic diversity and population structure of the C. gloeosporioides populations. Information obtained from this study could be useful as a base to design strategies for better management of leaf and fruit spot disease of citrus in Ethiopia.

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Section: Introductionmentioning

confidence: 99%

Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia

et al. 2016

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Analysis of microsatellite markers in the genome of the plant pathogen Ceratocystis fimbriata

et al. 2013

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Ceratocystis fimbriata sensu lato represents a complex of cryptic and commonly plant pathogenic species that are morphologically similar. Species in this complex have been described using morphological characteristics, intersterility tests and phylogenetics.Microsatellite markers have been useful to study the population structure and origin of some species in the complex. In this study we sequenced the genome of C. fimbriata. This provided an opportunity to mine the genome for microsatellites, to develop new microsatellite markers, and map previously developed markers onto the genome. Over 6000 microsatellites were identified in the genome and their abundance and distribution was determined. Ceratocystis fimbriata has a medium level of microsatellite density and slightly smaller genome when compared with other fungi for which similar microsatellite analyses have been performed. This is the first report of a microsatellite analysis conducted on a genome sequence of a fungal species in the order Microascales. Forty-seven microsatellite markers have been published for population genetic studies, of which 35 could be mapped onto the C. fimbriata genome sequence. We developed an additional ten microsatellite markers within putative genes to differentiate between species in the C. fimbriata s.l.complex. These markers were used to distinguish between 12 species in the complex.

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A new set of microsatellite markers for the genetic characterization of Ceratocystis fimbriata, an economically important plant pathogen

Cited by 2 publications

References 10 publications

Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia

Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia

Analysis of microsatellite markers in the genome of the plant pathogen Ceratocystis fimbriata

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