A New Serological Test (Inhibition Test) for Human Serum Globulin

A sequence of tests of minute human blood stains for human origin identification and ABO blood grouping

“…Immunoelectrosyneresis [10,11] and anti-human globulin inhibition test [12,13] are frequently used for identification of human origin of doubtful blood stains. In this paper, human blood was identified by the microprecipitation method which is able to detect several pg of hemoglobin in a single erythrocyte [14].…”

The Demonstration of Human Gm-Like Serum γ-Globulin Types Using Nonhuman Primate Reagents

“…For fuller details of the technique of the quantitative antiglobulin inhibition test, the original paper should be consulted [8],…”

“…This reagent was used for an inhibition test with rhesus monkey red cells coated with rhesus isoantibodies or baboon red cells coated with isoantibodies as indicator. With this test individual differences could be demon strated in human y-globulins comparable to the Gm types.The results to be described in this article were obtained as part of a study on the antiglobulin inhibition test [8]. The principle of this test is as follows: Antiglobulin immune serums are prepared by immunizing rabbits or nonhuman primates with human serum globulin or with the serum or purified serum globulin of a nonhuman primate animal, as the case may be.…”

A Two-Component System of Human Serum Agglutinating Gelatine-Coated Erythrocytes

“…Determination of human IgG in serum fractions was performed according to Wiener et al [22], Two-fold serial dilutions of a standard IgG preparation of known concentration and of the fraction under investigation were incubated with eight hemagglutinating doses antiserum against human IgG. Rh-positive red cells sensitized with anti-D antibodies were added and the inhibiting capacities of both preparations were compared.…”