A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Hollert, Henner; Keiter, Steffen; König, Natalie; Rudolf, Marc; Ulrich, Markus; Braunbeck, Thomas

doi:10.1007/bf02991051

Cited by 25 publications

(53 citation statements)

References 20 publications

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“…SPM extracts were also toxic when sampled at mean annual discharge directly after the flood event but became less toxic thereafter. Hollert et al (2003bHollert et al ( , 2007a showed that SPM from the 1998 flood event at the Neckar (HQ=20) caused NR 50 values for RTG-2 cells between 20-and 60-mg/mL test medium. These results were comparable to the toxicity caused by the 2004 flood SPM assessed in this study.…”

Section: Cytotoxic Effects Of Complex Samplesmentioning

confidence: 98%

Changes in toxicity and Ah receptor agonist activity of suspended particulate matter during flood events at the rivers Neckar and Rhine — a mass balance approach using in vitro methods and chemical analysis

Wölz

Engwall

Maletz

et al. 2008

Environ Sci Pollut Res

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As a consequence of climate change, flood events will increase in frequency and intensity at least in some regions such as Central Europe. Thus, it is crucial to identify the potential hazard of (re-)mobilized contaminants from reservoirs dislocated via floods and threatening especially aquatic organisms and cattle grazing in flood plains. Since other less persistent compounds seem to be more relevant to explain AhR-mediated activities in flood SPM, nonconventional PAHs and more polar compounds also need to be considered for risk assessment. Effect-directed analysis using broad-range fractionation methods taking into account compounds from polar to nonpolar should be applied for identification of pollutants causing biological effects, thus integrating biological and chemical parameters.

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Section: Cytotoxic Effects Of Complex Samplesmentioning

confidence: 98%

Changes in toxicity and Ah receptor agonist activity of suspended particulate matter during flood events at the rivers Neckar and Rhine — a mass balance approach using in vitro methods and chemical analysis

Wölz

Engwall

Maletz

et al. 2008

Environ Sci Pollut Res

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“…Even though the MariETT is a laboratory-based assay focusing on toxicological endpoints of a selected model species, future application is envisaged to include testing of ''natural'' samples such as stream water or sediment interstitial water. Similar to the zebrafish, Danio rerio embryo toxicity test (DarT) which also has started as a pure toxicological test with single substances but now is even used for natural sediment assessment (Hollert et al 2003;Hallare et al 2005a, b;Wu et al 2010), we see a great potential of the MariETT for ecotoxicological application in the future.…”

Section: Discussionmentioning

confidence: 97%

Metal sensitivity of the embryonic development of the ramshorn snail Marisa cornuarietis (Prosobranchia)

Sawasdee

Köhler

2010

Ecotoxicology

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We investigated the effects of metal ions on the embryonic development of the ramshorn snail, Marisa cornuarietis, by exposing embryos to varying concentrations of copper (0, 50, 100, and 250 μg Cu(2+)/L), lead (0, 5, 10, and 15 mg Pb(2+)/L), lithium (0, 1, 2.5, and 3 mg Li(+)/L), or palladium (0, 50, 100, and 500 μg Pd(2+)/L). Effects of these metals were examined by recording mortality, the rate of tentacles and eyes formation, heart rate, hatching success, and weight after hatching. Compared to the control, we found a significant delay in the formation of tentacles and eyes after treatment with 100 μg Cu(2+)/L, 15 mg Pb(2+)/L, 2.5 mg Li(+)/L or 500 μg Pd(2+)/L. The heart rate decreased significantly at 500 μg Pd(2+)/L. At 10 mg Pb(2+)/L, 2.5 mg Li(+)/L, or 500 μg Pd(2+)/L, hatching was delayed significantly; 50 μg Cu(2+)/L induced a significantly earlier hatching, and reduced body weight. The LC(50) values were calculated to be about 50 μg Cu(2+)/L, 500 μg Pd(2+)/L, 2500 μg Li(+)/L, and 10000 μg Pb(2+)/L. These results show that the embryonic development of M. cornuarietis is about as sensitive to copper and lithium, compared to the most sensitive fishes used in embryo toxicity testing. Even though the MariETT is a laboratory-based assay focusing on toxicological endpoints of a selected model species, future application is envisaged to include testing of "natural" samples such as stream water or sediment interstitial water.

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“…Sediments represent one of the ultimate sinks for organic and inorganic contaminants including heavy metals discharged into environment (Bettinetti et al 2003;Hollert et al 2003;Luoma and Bryan 1981). Sediments also play a significant role in the remobilization of contaminants (Zhigang and Gao 2007).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of heavy metal enrichment in Cochin estuary and its adjacent coast: multivariate statistical approach

Bindu

Deepulal

Gireeshkumar

et al. 2015

Environ Monit Assess

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Temporal and spatial variations of heavy metals in the Cochin estuary and its adjacent coastline during three seasons were studied to investigate the impact of anthropogenic heavy metal pollution. Total organic carbon, sand, silt, clay and 10 metals (Cd, Pb, Cr, Ni, Co, Cu, Zn, Mn, Mg and Fe) in the surface sediments were analysed. Multivariate statistical analyses like canonical correspondence analysis, principal component analysis and cluster analysis were used for source identification, integration of geochemical data and clustering of stations based on similarities. Enrichment factor, contamination factor and geoaccumulation index were used to assess the contamination level. From the study, it can be understood that estuary and coast are highly polluted especially with Cd, Zn, Pb and Ni. Anthropogenic influence of heavy metals was evidenced from both the principal component analysis and cluster analysis. Finer fractions (mud) of the sediment and the associated Fe oxy hydroxides might be playing major role in the transport of heavy metals in the system. Very high enrichment factor value observed suggested high anthropogenic pressure in the study area. All the stations in the northern part of the estuary showed very high enrichment factors indicating heavy load of Zn and Cd in this area which might have reached from the industrial area lying to the north side of the Cochin estuary. Pollution indices suggested that both the estuary and its adjacent coast were showing low contamination with respect to Cr, Mg, Mn and Fe; all other metals were causing low to extremely high levels of pollution in the study area.

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A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos

Cited by 25 publications

References 20 publications

Changes in toxicity and Ah receptor agonist activity of suspended particulate matter during flood events at the rivers Neckar and Rhine — a mass balance approach using in vitro methods and chemical analysis

Changes in toxicity and Ah receptor agonist activity of suspended particulate matter during flood events at the rivers Neckar and Rhine — a mass balance approach using in vitro methods and chemical analysis

Metal sensitivity of the embryonic development of the ramshorn snail Marisa cornuarietis (Prosobranchia)

Evaluation of heavy metal enrichment in Cochin estuary and its adjacent coast: multivariate statistical approach

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