A New Screening Method for the Detection of Antibiotic Residues in Meat and Poultry Tissues

Johnston, Richard H.; Reamer, R. H.; Harris, Elmer W.; Fugate, Harold G.; Schwab, Bernard

doi:10.4315/0362-028x-44.11.828

Cited by 37 publications

(16 citation statements)

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“…The possibility for preliminary identification from activity profiles was also explored for the US Food Safety and Inspection Service (USDA-FSIS) method [ 25 , 26 ]. This method consists of seven test plates and is used by the USDA-FSIS as a microbial confirmatory procedure for samples which tested positive in initial screening tests such as STOP [ 27 ], CAST [ 28 ], and FAST [ 29 ]. The method does not use the commonly applied K. rhizophila ATCC 9341, but uses two erythromycin- or (dihydro)streptomycin-resistant derivatives, which may improve the identification of macrolides and aminoglycosides.…”

Section: Methods Developmentmentioning

confidence: 99%

Microbial screening methods for detection of antibiotic residues in slaughter animals

Pikkemaat

2009

Anal Bioanal Chem

131

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Monitoring of food products from animal origin for the presence of antimicrobial residues is preferably done using microbial screening methods because of their high cost-effectiveness. Traditionally applied methods fail to detect the maximum residue limits which were established when EU Council Regulation 2377/90 came into effect. Consequently, during the last decade this has led to the development of improved microbial screening methods. This review provides an overview of the efforts expended to bring antibiotic screening methods into compliance with EU legislation. It can be concluded that the current situation is still far from satisfactory.FigureMicrobial inhibition assay for muscle samples

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Section: Methods Developmentmentioning

confidence: 99%

Microbial screening methods for detection of antibiotic residues in slaughter animals

Pikkemaat

2009

Anal Bioanal Chem

131

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“…Microbiological (bioassay) methods that are used for the detection of antibiotics in animal tissues rely on their ability to inhibit the growth of sensitive bacteria (Johnston et al, 1981). It is a simple inhibition test that uses one test bacterium and one medium.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of fluoroquinolone residual levels in chicken eggs by microbiological assay and confirmation by liquid chromatography

Cho

El‐Aty

Goudah

et al. 2007

Biomedical Chromatography

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The primary objective of this study was to develop a simple, rapid, and efficient method for the simultaneous determination of four fluoroquinolone residues, ciprofloxacin (CFX), danofloxacin (DFX), enrofloxacin (EFX) and norfloxacin (NFX), in chicken eggs. The samples were first monitored by microbiological assay using Escherichia coli as the reference organism, and were then quantified using HPLC with a fluorescence detector. Egg samples were extracted by the liquid-phase extraction process, and the analytes were analyzed via an ODS column using a mixture of acetonitrile and 0.4% phosphoric acid-0.4% triethylamine (15: 85, v/v) as a mobile phase (pH=2) without purification. The calibration curves were linear (r2>or=0.999) over a concentration range of 0.1-1.0 microg/mL. The majority of the mean recoveries at four different fortification levels, 0.1, 0.2, 0.5 and 1.0 ppm, ranged from 73.7+/-7.2% to 87.1+/-12.7%, and the repeatability (as the relative standard deviation) from three repetitive determinations of recovery was between 1.03 and 18.83%. The calculated limit of quantitation (LOQ) was 9 ppb for CFX, EFX and NFX and 0.6 ppb for DFX. Both the bioassay and HPLC methods were applied to 120 total egg samples collected from the six major cities in the Republic of Korea. The bioassay, showed that two samples were positive (i.e contained inhibiting substances). On the other hand, the results of HPLC only identified and quantified the residues of enrofloxacin (from 0.43 to 1.02 ppm) in three samples out of 120. We concluded that the bioassay can be used as a routine screening method for the presence of fluoroquinolones in chicken eggs, which can be confirmed and quantified using LC.

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“…In the 1970's, the USDA began testing for residual antibiotics in meat and poultry [10] by slightly modifying a FDA procedure used for testing antibiotic residues in milk. [25] The samples collected at the slaughter establishments were analyzed in a FSIS laboratory for violative levels of antibiotic residues.…”

Section: Discussionmentioning

confidence: 99%

“…They include the Swab Test on Premises, [9,10] the Calf Antibiotic and Sulfonamide Test [11] and the Fast Antimicrobial Screen Test, [12] which have been used for monitoring and surveillance testing. They include the Swab Test on Premises, [9,10] the Calf Antibiotic and Sulfonamide Test [11] and the Fast Antimicrobial Screen Test, [12] which have been used for monitoring and surveillance testing.…”

Section: Introductionmentioning

confidence: 99%

“…The performance of all these screen tests has been reported, [13 -15] their use reviewed [16,17] and their impact on the violation rate in food animal carcasses with agricultural residue has been reported. [10] In 1980, the test was modified for use in slaughter establishments by trained FSIS inspectors. [10] In 1980, the test was modified for use in slaughter establishments by trained FSIS inspectors.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Microbiological Screen Test Data for Antimicrobial Residues in Food Animals

Dey

Thaler

Gwozdz

2003

Journal of Environmental Science and Health, Part B

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This study analyzes the National Residue Program (NRP) of the Food Safety and Inspection Service (FSIS), United States Department of Agriculture (USDA), data for the years 1983-1998 to determine the effectiveness of all three microbiological screen tests that were developed and used by the FSIS to control antimicrobial residues in food animals. The Swab Test On Premises (STOP) was the first screen test introduced in slaughterhouses, followed by the Calf Antibiotic Sulfonamide Test (CAST) and the Fast Antimicrobial Screen Test (FAST). The data for STOP indicates that during 1983-1998, the rate of food animal carcasses with violative levels of antimicrobial residues reduced from 2.33% to 0.45% under the monitoring plan and under the surveillance plan, the rate reduced from 55.1% to 0.56%. Similarly, the data for CAST indicates that the rate of calf carcasses with violative levels of antimicrobial residue also declined significantly during those years. Because of its higher sensitivity and shorter analytical time, the use of FAST started in 1995. By 1999, it had practically replaced the use of STOP and CAST in bovine species. The use of only one test such as FAST instead of different tests has removed confusion for testing different species of food animals and thereby has enhanced the efficiency of the NRP.

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A New Screening Method for the Detection of Antibiotic Residues in Meat and Poultry Tissues

Cited by 37 publications

References 0 publications

Microbial screening methods for detection of antibiotic residues in slaughter animals

Microbial screening methods for detection of antibiotic residues in slaughter animals

Monitoring of fluoroquinolone residual levels in chicken eggs by microbiological assay and confirmation by liquid chromatography

Analysis of Microbiological Screen Test Data for Antimicrobial Residues in Food Animals

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