A new <scp>open‐source</scp> hardware device to measure vertical sperm motility and concentration

Arzaluz, Cindy Rivas; Ayala, Marı́a Elena; Martínez, Andrés

doi:10.1002/cyto.a.24343

Cited by 6 publications

(4 citation statements)

References 37 publications

(56 reference statements)

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“…We captured each image sequence with µManager version 1.4 software (Edelstein et al, 2014) at 60 frames per second (60 Hz) for two seconds. We looked at three to four fields per sample and analysed the image sequences using ImageJ software version 1.50d (Rasband, 1997) and the plugin developed by Wilson-Leedy and Ingermann (2007) and modified by Giaretta et al (2017). We modified the settings of the plugin (CASA_bgm) for use with boar sperm.…”

Section: Methodsmentioning

confidence: 99%

Effect of various pH levels on the sperm kinematic parameters of boars

Rivas¹,

Ayala²,

Martínez³

2023

SA J. An. Sci.

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The objective of this work was to identify the effects of pH on the structure of kinematic sperm subpopulations using commercial doses of boar semen diluted in Androstar Plus extender. We adjusted the pH value of the diluted semen samples to 7.4, 7.6, 7.8, and 8.0, and evaluated motility parameters using an open-source computer-assisted sperm analysis system. We evaluated the dataset of the kinematic parameters of the individual sperm for the effects of pH on the average values overall and on the subpopulations of sperm. To identify these subpopulations, we reduced the dimensionality of the dataset through principal component analysis followed by hierarchical clustering. We then compared the results for the kinematic parameters across the dataset and in the subpopulations with the average of the corresponding control (i.e., the basal pH value). We found that increasing the pH did not affect the proportion of motile sperm but did affect the manner in which they moved, rendering their movement more linear. The progressive motility increased relative to the control at all pH values above 7.1, while the proportion of sperm with low motility increased at pH 7.8. We distinguished three sperm subpopulations. The proportion of sperm in Subpopulation 1 increased at pH values of 7.8 and 8.0, with the sperm being characterized by low velocity and high values for linearity. The sperm in Subpopulation 3, by contrast, were relatively few but showed relatively high curvilinear velocity. In this study, we demonstrated the presence of subpopulations of boar sperm that responded differentially to changes in pH.

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Section: Methodsmentioning

confidence: 99%

Effect of various pH levels on the sperm kinematic parameters of boars

Rivas¹,

Ayala²,

Martínez³

2023

SA J. An. Sci.

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“…Moreover, CASA's versatility has been demonstrated by its use in identifying distinct subpopulations of motile spermatozoa in donkey ejaculates (Catalán et al 2020). According to Arzaluz et al (2021), CASA systems evaluate sperm motility in the horizontal plane on the monitor, providing precise details on sperm motion characteristics.…”

Section: Kinematics Of Fresh and Frozen Ram Spermmentioning

confidence: 99%

Quality and Protein Profiles in Local Indonesian Ram Sperm Before and After Cryopreservation

2024

Int J Vet Sci

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Sperm quality plays a crucial role in determining the success of fertilization. This study aimed to assess the quality of both fresh and frozen ram semen, as well as examine changes in semen protein content. Four local rams, aged 3-4 years, were selected for the study. Semen collection was conducted in the morning using an artificial vagina. The collected semen was divided into two parts: one for analyzing fresh semen and the other for freezing. The freezing process involved using a diluent consisting of Tris hydroxy amino methane, citric acid, egg yolk fructose, 20% egg yolk, and glycerol. Both fresh and frozen semen samples underwent analysis using the same method, including examination of concentration, motility, viability, and various kinematic motility parameters, such as VAP, VCL, VSL, LIN, and STR, with the assistance of CASA (AndroVision®). Following collection, both samples were centrifuged at 4500rpm for 10min, and the supernatant and precipitate were stored at -40°C until analysis. Protein concentration was determined using the Bradford kit, and semen protein profiles were evaluated through SDS-PAGE and Coomassie Blue staining. The study revealed a significant decrease in semen quality following cryopreservation (P<0.05). Protein analysis results indicated that fresh semen exhibited ten specific proteins, whereas frozen semen expressed six specific proteins. These findings suggested a correlation between protein content and sperm quality, with the cryopreservation process altering the composition of proteins in sperm.

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“…Portable analysis devices are beginning to be developed so that semen quality analysis can be performed directly at the shelter location to maintain its quality. An Arduino Uno-based device has been developed to analyze spermatozoa motility and concentration vertically, with the working principle of measuring absorbance using a TSL2561 luminosity sensor on semen in a cuvette [6]. The bull semen quality analysis device developed in this study was based on an Arduino Mega 2560 with four sensor systems to access semen quality parameter data including volume, viscosity, pH, and colour.…”

Section: Introductionmentioning

confidence: 99%

Automation of bull semen analysis through the development of a modular open technology device for cryopreservation

Prabaswara,

Aryawan,

Haikal

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Bull semen quality analysis is an important step before processing frozen semen for artificial insemination. Transportation from the shelter to the laboratory can reduce semen quality and kill the spermatozoa. This study aimed to develop a prototype that can automatically analyse bull semen quality using a modular open-technology device with multi-digital sensors that is comparable to standard laboratory measurements. The prototype components consisted of the following: (1) input: syringe as the semen container to be tested, do-it-yourself (DIY) water level sensor for volume measurement, Total Dissolve Solid (TDS) sensor for viscosity measurement, pH sensor for pH value measurement, and Light Dependent Resistor (LDR) for colour measurement; (2) process: Arduino Mega 2560 as a microcontroller; and (3) output: Liquid Crystal Display (LCD) 16x2 for displaying the measurement. The results showed that the coefficients of determination for the volume, TDS, and pH sensors were 1, 0.9993, and 0.9987, respectively. Data from the TDS and LDR sensor calibration results showed three viscosity levels (low: 300-599 ppm, moderate: 600-999 ppm, and high: 1.000-1.400 ppm) and three colour quality values (cloudy white: 40-59 Ω, milky white: 60-89 Ω, and creamy white: 90-130 Ω). The device developed was able to analyse semen quality in less than 5s and was displayed on an LCD screen.

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A new open‐source hardware device to measure vertical sperm motility and concentration

Cited by 6 publications

References 37 publications

Effect of various pH levels on the sperm kinematic parameters of boars

Effect of various pH levels on the sperm kinematic parameters of boars

Quality and Protein Profiles in Local Indonesian Ram Sperm Before and After Cryopreservation

Automation of bull semen analysis through the development of a modular open technology device for cryopreservation

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