A new role for OPG: Putting RANKL in its place

Ostrowski, Michael C.

doi:10.1002/jbmr.206

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…They showed that LAMP-2A has a critical role in recruitment of the co-factors synaptosomal associated protein-29 (SNAP-29) and vacuolar protein sorting-associated proteins 33a (Vps33a) which are important for the fusion of lysosomes and autophagosomes. Moreover, others have shown that most of the newly synthesized RANKL is not immediately transported to the plasma membrane, but stored in secretory lysosomes [ 19 , 38 , 39 ]. The latter authors hypothesized that two pathways exist for RANKL transport to the membrane: a direct minor pathway and an indirect major pathway via secretory lysosomes.…”

Section: Discussionmentioning

confidence: 99%

LAMP-2 Is Involved in Surface Expression of RANKL of Osteoblasts In Vitro

Jansen

Tigchelaar-Gutter

Hogervorst

et al. 2020

IJMS

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Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential. Osteoclast precursors express RANK on their membrane and RANKL is expressed by cells of the osteoblast lineage. Recently it has been suggested that the transport of RANKL to the plasma membrane is mediated by lysosomal organelles. We wondered whether LAMP-2 might play a role in transportation of RANKL to the plasma membrane of osteoblasts. To elucidate the possible function of LAMP-2 herein and in the formation of osteoclasts, we analyzed these processes in vivo and in vitro using LAMP-2-deficient mice. We found that, in the presence of macrophage colony stimulating factor (M-CSF) and RANKL, active osteoclasts were formed using bone marrow cells from calvaria and long bone mouse bone marrow. Surprisingly, an almost complete absence of osteoclast formation was found when osteoclast precursors were co-cultured with LAMP-2 deficient osteoblasts. Fluorescence-activated cell sorting FACS analysis revealed that plasma membrane-bound RANKL was strongly decreased on LAMP-2 deficient osteoblasts. These results suggest that osteoblastic LAMP-2 is required for osteoblast-induced osteoclast formation in vitro.

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Section: Discussionmentioning

confidence: 99%

LAMP-2 Is Involved in Surface Expression of RANKL of Osteoblasts In Vitro

Jansen

Tigchelaar-Gutter

Hogervorst

et al. 2020

IJMS

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“…On the other hand, cells have an important role because cells together with extracellular matrix (ECM) molecules act as biological triggers that will stimulate endogenous regeneration. The goal is to develop cells in the scaffold over a period of time which will then build a network of scaffolds / cells to be implanted in vivo 7,8 .…”

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confidence: 99%

OPG and RANKL Signal Transduction in Osteoblast Regulation Post Application Extract Collagen in Osteogenesis

Kurnia

Prahasanti

Hendro

et al. 2022

RJPT

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Background and Aim: The current goal of periodontal therapy is to achieve periodontal regeneration. Important factor for periodontal regeneration is to promote bone formation, nowadays used bone replacement. The used of xenograft materials as gold standard for periodontal tissue regeneration using type I collagen bone graft has been widely developed. The main organic component in fish scales is type I fibril collagen, which are same as with component in bones. Specific markers of bone formation are the expression of osteoblast, osteoclast, osteoprotegerin (OPG), and receptor activator of nuclear factor κB ligands (RANKL). The purpose of this study is to assess the expression of OPG and RANKL after application of extract collagen from gourami (Osphronemus gouramy) scales. Materials and Methods: Thirty-two experimental 3-month-old male Wistar albino rats (150g and 200g) were randomly divided into four groups: 7-day control group, 7-day fish collagen group, 14-day control group and 14-day fish collagen group. The sockets were filled with blood (control group), whereas 10 mg to 30 mg extract collagen was added until the sockets were fully occupied (treatment group). Results: The ANOVA test showed a significance level of 0.000 (p <0.05). Conclusion: Expression of OPG enhanced and expression of RANKL lowered after application of type I collagen from gourami scales, accelerating osteogenesis.

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A new role for OPG: Putting RANKL in its place

Cited by 2 publications

References 7 publications

LAMP-2 Is Involved in Surface Expression of RANKL of Osteoblasts In Vitro

LAMP-2 Is Involved in Surface Expression of RANKL of Osteoblasts In Vitro

OPG and RANKL Signal Transduction in Osteoblast Regulation Post Application Extract Collagen in Osteogenesis

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