A new role for complement C3: Regulation of antigen processing through an inhibitory activity

Villiers, Christian; Cretin, François; Lefebvre, N.; Marche, Patrice N.; Villiers, Marie‐Bernadette

doi:10.1016/j.molimm.2008.05.015

Cited by 5 publications

(5 citation statements)

References 47 publications

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“…The most affected gene affected by XPC supplementation, complement component 3 (C3), was sevenfold upregulated over the control group. C3 also plays a role in B-cell activation and is thought to facilitate adaptive immunity (12, 13). Several germline-encoded innate immune sensors are among the significantly modified genes of the XPC group, including toll-like receptors ( TLRs ), interferon induced with helicase C domain 1 ( IFIH1 ), and nucleotide-binding oligomerization domain containing 1 ( NOD1 ) (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory Effects of Saccharomyces cerevisiae Fermentation Product Supplementation on Immune Gene Expression and Lymphocyte Distribution in Immune Organs in Broilers

et al. 2017

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A study was conducted to evaluate the molecular and cellular immunomodulatory effects of a Saccharomyces cerevisiae fermentation product (Original XPC, Diamond V) in broilers. Our lab has previously demonstrated that broilers fed XPC generate faster and stronger antigen-specific humoral immune responses to Newcastle disease virus (NDV) vaccination. This study aims at investigating the mechanism behind this increased immunocompetence. One-day-old broilers were randomly assigned to one of two treatments: 1.25 kg/ton S. cerevisiae fermentation product (XPC treatment group) or control diet. Birds were vaccinated against NDV on day 1 (B1 strain) and day 21 (LaSota strain) post-hatch. Innate and adaptive immune-related gene expression profiles in central (thymus and bursa of Fabricius) and peripheral (spleen) immune organs were investigated at 14 and 28 days of age by qPCR array. Fold changes larger than 1.2 (P < 0.05) between treated and control were considered significant. Lymphocyte subpopulations in central and peripheral immune organs and blood leukocytes were analyzed by flow cytometry at 14, 21, 28, and 42 days of age. In the spleen, Th1 immune responses and antiviral genes, such as IFN-γ, and its downstream genes signal transducer and activator of transcription (STAT4) and NFκB, were significantly upregulated in the treated group by 14 days of age. In the thymus, genes belonging to different functional groups were influenced at different time points. Cytokine genes associated with lymphocyte maturation, differentiation, and proliferation, such as IL-1R, IL-2, and IL-15 were significantly upregulated in the treated group by 28 days of age. Genes preferentially expressed in the medulla of the thymus and mature thymocytes, such as Myxovirus resistance gene 1, interferon regulatory factor-1, interferon regulatory factor-7, and STAT1, were upregulated in the birds supplemented with XPC. Birds supplemented with XPC had significantly higher percentages of CD3+, CD4+, and CD8+ T-cells in the thymus at day 28 of age, indicating production of more mature T-cells, which was consistent with gene expression results. Results suggest that XPC supplementation primes broilers to become more immunocompetent, without compromising growth performance.

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Section: Resultsmentioning

confidence: 99%

Immunomodulatory Effects of Saccharomyces cerevisiae Fermentation Product Supplementation on Immune Gene Expression and Lymphocyte Distribution in Immune Organs in Broilers

et al. 2017

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“…It has been reported that C3b and C3c can also control antigen proteolysis, which could be related to C3-mediated enhancement of antigen presentation, thus, aiding in the establishment of a specific adaptive immune response 52 .…”

Section: Discussionmentioning

confidence: 99%

“…[49][50][51] It has been reported that C3b and C3c can also control antigen proteolysis, which could be related to C3mediated enhancement of antigen presentation, thus aiding in the establishment of a specific adaptive immune response. 52 Complement components C5b, C6, C7, C8 and C9 create the MAC. Out of these five proteins, we observed up-regulation of C6, C8 and C9, as well as C5, which is directly upstream of this complex.…”

Section: Discussionmentioning

confidence: 99%

“…Complement C3 also modulates adaptive immunity at various levels, including cytokine release, T-cell proliferation, regulatory T cell development and B cell activation/differentiation. − It has been reported that C3b and C3c can also control antigen proteolysis, which could be related to C3-mediated enhancement of antigen presentation, thus aiding in the establishment of a specific adaptive immune response …”

Section: Discussionmentioning

confidence: 99%

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Plasma Proteomic Analysis of Simian Immunodeficiency Virus Infection of Rhesus Macaques

et al. 2010

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Lentiviral replication in its target cells affects a delicate balance between cellular co-factors required for virus propagation and immunoregulation for host defense. To better elucidate cellular proteins linked to viral infection we tested plasma from rhesus macaques infected with the simian immunodeficiency viral strain SIVsmm9, prior to, 10 days (acute) and 49 weeks (chronic) after viral infection. Changes in plasma protein content were measured by quantitative mass spectrometry by isobaric Tags for Absolute and Relative Quantitation (iTRAQ) methods. An 81 and 232% increase in SERPINA1 was seen during acute and chronic infection, respectively. Interestingly, gelsolin, vitamin D binding protein and histidine rich glycoprotein were decreased by 45% in acute conditions but returned to baseline during chronic infection. When compared to uninfected controls, a 48–103% increase in leucine rich alpha 2-glycoprotein, vitronectin and ceruloplasmin was observed during chronic viral infection. Observed changes in plasma proteins expression likely represent a compensatory host response to persistent viral infection.

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“…These pathways converge to a unique sequence of events: the formation of the membrane attack complex (MAC) responsible for membrane lysis [6], [7]. In addition to its role in the innate immunity acting directly on microorganisms, the complement system plays an important role in the normal function of the adaptive immune system, contributing markedly to antigen presentation that makes the humoral response much more efficient [7]–[10].…”

Section: Introductionmentioning

confidence: 99%

The Role of Salivary and Intestinal Complement System Inhibitors in the Midgut Protection of Triatomines and Mosquitoes

et al. 2009

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Saliva of haematophagous arthropods contain biomolecules involved directly or indirectly with the haematophagy process, and among them are encountered some complement system inhibitors. The most obvious function for these inhibitors would be the protection of the midgut against injury by the complement. To investigate this hypothesis, Triatoma brasiliensis nymphs were forced to ingest human serum in conditions in which the protection of midgut by the inhibitors is bypassed. In these conditions, the anterior midgut epithelium was injured by the complement, causing cell death. Once some insects such as Aedes aegypti have no salivary inhibitors, we hypothesized the existence of intestinal inhibitors. The inhibitory activity was investigated in the intestine of A. aegypti as well as in the saliva and intestine of other three triatomine species (T. brasiliensis, T. infestans and Rhodnius prolixus) using an immunological method able to determine the level of deposition of some complement factors (C1q, C3b, or C4b) on the surface of complement activator molecules linked to microplates. This methodology permitted to identify which points along the activation phase of the complement cascade were inhibited. As expected, soluble contents of A. aegypti's intestine was capable to inhibit C3b deposition by the classical and alternative pathways. Saliva or soluble intestinal contents, obtained from triatomines were unable to inhibit C1q deposition by the classical pathway. C4b deposition by the classical pathway was inhibited by the intestinal contents from the three triatomines. On the other hand, only T. brasiliensis saliva inhibited C4b deposition. Both, saliva and intestinal contents from all triatomines were able to inhibit C3b deposition in the classical and alternative pathways. None of the material extracted from the intestinal cell membranes from the triatomines inhibited C3b deposition in the classical pathway. The existence of complement inhibitors may have important biological consequences which are discussed in detail.

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A new role for complement C3: Regulation of antigen processing through an inhibitory activity

Cited by 5 publications

References 47 publications

Immunomodulatory Effects of Saccharomyces cerevisiae Fermentation Product Supplementation on Immune Gene Expression and Lymphocyte Distribution in Immune Organs in Broilers

Immunomodulatory Effects of Saccharomyces cerevisiae Fermentation Product Supplementation on Immune Gene Expression and Lymphocyte Distribution in Immune Organs in Broilers

Plasma Proteomic Analysis of Simian Immunodeficiency Virus Infection of Rhesus Macaques

The Role of Salivary and Intestinal Complement System Inhibitors in the Midgut Protection of Triatomines and Mosquitoes

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