A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in
            <i>Arabidopsis</i>
            <i>thaliana</i>

Desikan, Radhika; Griffiths, Rachael; Hancock, John T.; Neill, Steven J.

doi:10.1073/pnas.252461999

Cited by 625 publications

(570 citation statements)

References 33 publications

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“…Our data also suggest that ABA and H 2 O 2 could induce the activity of NOS, which is primarily located in the microsomal fraction, in different subcellular compartments. In previous studies, however, it was reported that NR was the main source of NO production in response to ABA and H 2 O 2 in Arabidopsis guard cells [38,45]. The results described above suggest that there are different sources of NO in response to ABA and H 2 O 2 in guard cells and mesophyll cells.…”

Section: Discussionmentioning

confidence: 74%

Cross-talk between calcium-calmodulin and nitric oxide in abscisic acid signaling in leaves of maize plants

et al. 2008

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Section: Discussionmentioning

confidence: 74%

Cross-talk between calcium-calmodulin and nitric oxide in abscisic acid signaling in leaves of maize plants

et al. 2008

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“…However, the relationship between NO 3 2 and ABA has been opaque. Genetic analysis placed ABA signaling downstream of NO 3 2 perception in the regulation of root architecture (Signora et al, 2001;Zhang et al, 2007), but upstream of nitric oxide production, in several physiological processes (Desikan et al, 2002;León et al, 2014). The link between environmental NO 3 2 and the triggering of ABA signaling within the root, however, has just begun to be developed.…”

Section: Nomentioning

confidence: 99%

Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores

2016

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Abscisic acid (ABA) signaling plays a major role in root system development, regulating growth and root architecture. However, the precise localization of ABA remains undetermined. Here, we present a mechanism in which nitrate signaling stimulates the release of bioactive ABA from the inactive storage form, ABA-glucose ester (ABA-GE). We found that ABA accumulated in the endodermis and quiescent center of Arabidopsis thaliana root tips, mimicking the pattern of SCARECROW expression, and (to lower levels) in the vascular cylinder. Nitrate treatment increased ABA levels in root tips; this stimulation requires the activity of the endoplasmic reticulum-localized, ABA-GE-deconjugating enzyme b-GLUCOSIDASE1, but not de novo ABA biosynthesis. Immunogold labeling demonstrated that ABA is associated with cytoplasmic structures near, but not within, the endoplasmic reticulum. These findings demonstrate a mechanism for nitrate-regulated root growth via regulation of ABA accumulation in the root tip, providing insight into the environmental regulation of root growth.

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“…The concentration of the plant stress hormone ABA increases under drought and induces stomatal closure through second messengers, including ROS, cytosolic Ca 2+ and NO [20][21][22] . We measured stomatal responses to ABA, hydrogen peroxide (H 2 O 2 ), NO and repetitive Ca 2+ pulses (Fig.…”

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confidence: 99%

SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling

Vahisalu

Kollist

Wang

et al. 2008

Nature

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Stomatal pores, formed by two surrounding guard cells in the epidermis of plant leaves, allow influx of atmospheric carbon dioxide in exchange for transpirational water loss. Stomata also restrict the entry of ozone-an important air pollutant that has an increasingly negative impact on crop yields, and thus global carbon fixation 1 and climate change 2 . The aperture of stomatal pores is regulated by the transport of osmotically active ions and metabolites across guard cell membranes 3,4 . Despite the vital role of guard cells in controlling plant water loss 3,4 , ozone sensitivity 1,2 and CO 2 supply 2,5-7 , the genes encoding some of the main regulators of stomatal movements remain unknown. It has been proposed that guard cell anion channels function as important regulators of stomatal closure and are essential in mediating stomatal responses to physiological and stress stimuli 3,4,8 . However, the genes encoding membrane proteins that mediate guard cell anion efflux have not yet been identified. Here we report the mapping and characterization of an ozone-sensitive Arabidopsis thaliana mutant, slac1. We show that SLAC1 (SLOW ANION CHANNEL-ASSOCIATED 1) is preferentially expressed in guard cells and encodes a distant homologue of fungal and bacterial dicarboxylate/malic acid transport proteins. The plasma membrane protein SLAC1 is essential for stomatal closure in response to CO 2 , ©2008 Nature Publishing GroupCorrespondence and requests for materials should be addressed to J.K. (jaakko.kangasjarvi@helsinki.fi). † Present address: Division of Biology, Imperial College London, London SW7 2AZ, UK. * These authors contributed equally to this work Fig. 3d and Supplementary Fig. 6a. N.N. performed experiments in Fig. 3a and Supplementary Fig. 7. Y.-F.W. performed experiments in Fig. 4 and Supplementary Figs 8 and 9. J.K. and J.I.S. wrote the paper. All the authors discussed the results, and commented on and edited the manuscript.The primary microarray data reported has been deposited with the ArrayExpress database under accession number E-MEXP-1388.Reprints and permissions information is available at www.nature.com/reprints. 8,11 by mediating anion efflux and causing membrane depolarization, which controls K + efflux through K + channels. So far, none of the candidates for plant anion channels -the plant homologues to the animal CLC chloride channels -has been localized to the plasma membrane 10 , and the first plant CLC channel that was functionally characterized encodes a central vacuolar proton/nitrate exchanger 12 , rather than an anion channel. Thus, despite their proposed importance in several physiological and stress responses in plants 8,10,11 , the molecular identity of the guard cell plasma membrane proteins that mediate anion channel activity has remained unknown. NIH Public AccessIn a mutant screen for O 3 sensitivity, a series of Arabidopsis ethyl methanesulphonate (EMS) mutants called radical-induced cell death (rcd) was identified 13,14 . One of them, a recessive mutant originally referred ...

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A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsis thaliana

Cited by 625 publications

References 33 publications

Cross-talk between calcium-calmodulin and nitric oxide in abscisic acid signaling in leaves of maize plants

Cross-talk between calcium-calmodulin and nitric oxide in abscisic acid signaling in leaves of maize plants

Environmental Nitrate Stimulates Abscisic Acid Accumulation in Arabidopsis Root Tips by Releasing It from Inactive Stores

SLAC1 is required for plant guard cell S-type anion channel function in stomatal signalling

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