A new RNA picorna — Like virus in the cotton pink bollwormPectinophora gossypiella (Lep.: Gelechiidae) in Egypt

Monsarrat, Annie; Abol-Ela, S.; Abdelhamid, Ismail A.; Fédière, Gilles; Kuhl, G.; Husseini, M. El; Giannotti, J.

doi:10.1007/bf02372680

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…The high HaNV abundance in the intestinal tract of both adult males and females (>90%), which could explain the observed low vertical-transmission efficiency of HaNV. Aggregation of infection within the intestinal tract is similar to the distributions observed for other picorna-like viruses, such as the Drosophila Nora virus (Habayeb et al, 2009) and a picorna-like virus in Pectinophora gossypiella (Monsarrat et al, 1995). However, it is a different distribution pattern to that of SeNV, which showed homogenous distribution in midgut, fat body and hemolymph (Jakubowska et al, 2014), and that of Iflavirus HaIV and densovirus HaDV2 in cotton bollworm, both mainly distributed in the fat body (Yuan et al, 2017).…”

Section: Within-host Distribution Of Hanv Infectionsupporting

confidence: 70%

Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

Yang

et al. 2019

Journal of Invertebrate Pathology

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We characterize a novel picorna-like virus, named Helicoverpa armigera Nora virus (HaNV), with a genome length of 11,200 nts, the sequence of which was isolated from the lepidopteran host cotton bollworm Helicoverpa armigera, using RNA-Seq. Phylogenetic analysis, using the putative amino acid sequence of the conserved RNA-dependent RNA polymerase (RdRp) domain, indicated that HaNV clustered with Spodoptera exigua Nora virus, Drosophila Nora virus and Nasonia vitripennis virus-3 with a high bootstrap value (100%), which might indicate a new viral family within the order Picornavirales. HaNV was efficiently horizontally transmitted between hosts via contaminated food, and transmission was found to be dose-dependent (up to 100% efficiency with 10 9 viral copy number/µl). HaNV was also found to be transmitted vertically from parent to offspring, mainly through transovum transmission (virus contamination on the surface of the eggs), but having a lower transmission efficiency (around 43%). Infection distribution within the host was also investigated, with HaNV mainly found in only the gut of both adult moths and larvae (>90%). Moreover, our results showed that HaNV appears not to be an overtly pathogenic virus to its host.

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Section: Within-host Distribution Of Hanv Infectionsupporting

confidence: 70%

Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

Yang

et al. 2019

Journal of Invertebrate Pathology

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“…In this study, we found the PBWs were infected by multiple RNA viruses. Picorna-like virus isolated from the pink bollworm population of Egypt was previously reported [8]. An Egyptian isolate of picorna-like virus caused severe pathogenetic impacts on the insects, specifically infection of the virus, resulting in death of the diseased larvae [8].…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the DNA virus, a small RNA virus (picoRNA-like virus) infecting pink bollworm larvae and adults and causing disease symptoms and even death of the infected larvae was isolated from cotton fields of Egypt. This virus infected midgut cells and could be vertically transmitted from infected adults to offspring [8]. However, genomic sequences of the RNA virus have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Novel RNA Viruses from the Transcriptome of Pheromone Glands in the Pink Bollworm Moth, Pectinophora gossypiella

Dou

Liu

Soroker

et al. 2021

Insects

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In this study, we analyzed the transcriptome obtained from the pheromone gland isolated from two Israeli populations of the pink bollworm Pectinophora gossypiella to identify viral sequences. The lab population and the field samples carried the same viral sequences. We discovered four novel viruses: two positive-sense single-stranded RNA viruses, Pectinophora gossypiella virus 1 (PecgV1, a virus of Iflaviridae) and Pectinophora gossypiella virus 4 (PecgV4, unclassified), and two negative-sense single-stranded RNA viruses, Pectinophora gossypiella virus 2 (PecgV2, a virus of Phasmaviridae) and Pectinophora gossypiella virus 3 (PecgV3, a virus of Phenuiviridae). In addition, sequences derived from two negative-sense single-stranded RNA viruses that belong to Mononegavirales were found in the data. Analysis of previous transcriptome sequencing data derived from the midgut of pink bollworm larvae of a USA population only identified PecgV1, but no other viruses. High viral sequence coverages of PecgV1 and PecgV4 were observed in both field and lab populations. This is the first report of viral sequences discovered from the pink bollworm. Results from this investigation suggest that the pink bollworm harbors multiple viruses. Further investigation of the viral pathogens may help to develop novel pest management strategies for control of the pink bollworm.

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“…In Egypt, cotton (Gossypium barbadens L.) is attacked by various pests during the different stages of its development; amongst, the most dangerous one is the pink bollworm P. gossypiella (PBW) (Salama, 1983). In Egypt, cotton field represents about 1/6 of the total cultivated area; heavy losses are yearly recorded due to the attack of PBW (Monsarrat et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

The Release of the Parasitoid Trichogramma evancens to Control the Pink Bollworm Pectinophora gossypiella (Saunders) and the Side-Effect of Certain Insecticides on It

2012

Alexandria Science Exchange Journal: An International Quarterly

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The present study was conducted to evaluate the effectiveness of the parasitoid Trichogramma evenecens (Hymenoptera, Trichogrammatidae) as a potential biological control agent upon the laid eggs of the pink bollworm Pectinophora gossypiella (Saunders) (PBW) in cotton fields. The effect of releasing Trichogramma evenecens, the application of the insecticide spinetoram (Radiant ® ) and their combination on the yield of cotton was considered. Meanwhile, the side-effect of certain insecticides on the parasitoid Trichogramma evanescens was involved. The toxic side-effects of these insecticides that have been recommended for controlling insect-pests attacking cotton plants namely gama-cyhalothrin, chlorpyrifos, spinetoram, methoxyfenozide and thiamethoxam were tested on the parasitized eggs of the Angoumois grain moth Sitotroga cerealella (Olvier).The results showed that the parasitoid T. evancens efficiently parasitized PBW Pectinophora gossypiella eggs. Parasitism percentage and the cotton yield were greatly affected by releasing T. evenecens at the rate of 33000 parasites/fed./release; thus decreasing the infestation of PBW and consequently increasing cotton yield. The efficiency of spinetoram was compared with the release of T. evanescens when they were separately used or when they were combined. The combination of releasing T. evanescens and application of spinetoram was superior to control PBW. Herein, the highest rate of infestation was recorded in the untreated check (9.3 and 15.5%); gradually decreased by the release of T. evanescens alone up to 3.4 and 5.0%, spinetoram alone (2.8 and 3.2%) and spinetoram with T. evanescens release (1.2 and 2.1%) in 2009 and 2010, respectively. The increase of cotton yield was the highest in case of releasing T. evanescens with the application of spinetoram during both seasons (8.5 and 8.2 Ken. / Fed., respectively). Chlorpyrifos and gamacyhalothrin were extremely toxic and adversely affected the exposed parasitized eggs of the grain moth S. cerealella (Olvier) since they caused complete inhibition (100%) of adults emergence in the treated parasitized egg of 48, 96 and 192 hrs old post-parasitisation, while more than 80% inhibition of adult emergence from the eggs of 24 hrs old was occurred. Methoxyfenozide was the least toxic and safer compound compared with the other tested ones.

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A new RNA picorna — Like virus in the cotton pink bollwormPectinophora gossypiella (Lep.: Gelechiidae) in Egypt

Cited by 8 publications

References 21 publications

Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

Discovery and characterization of a novel picorna-like RNA virus in the cotton bollworm Helicoverpa armigera

Novel RNA Viruses from the Transcriptome of Pheromone Glands in the Pink Bollworm Moth, Pectinophora gossypiella

The Release of the Parasitoid Trichogramma evancens to Control the Pink Bollworm Pectinophora gossypiella (Saunders) and the Side-Effect of Certain Insecticides on It

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