We have determined the mitochondrial genome of Reticulitermes speratus kyushuensis Morimoto, 1968. The total length of the R. speratus kyushuensis is 15,898 bp with 65.3% A þ T content. It consists of 13 PCGs, 22 tRNA, and 2 rRNA genes and an A þ T-rich control region. All the protein-coding genes used ATN as start codon. But the stop codons were TAA, TAG, and an incomplete termination codon (T) abutting an adjacent tRNA gene. The A þ T-rich control region was 1105 bp in length with 67.8% A þ T content.