A new recirculating enzymatic membrane reactor for ester synthesis in ionic liquid/supercritical carbon dioxide biphasic systems

Hernandez, Francisco Jose Arraez; Ríos, Antonia Pérez de los; Gómez, Demetrio; Rubio, Manuel Prieto; Víllora, Gloria

doi:10.1016/j.apcatb.2006.04.009

Cited by 77 publications

(28 citation statements)

References 21 publications

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“…On the other hand, the combination of SC-CO 2 and IL could also achieve higher reaction rate than IL alone by decreasing the viscosity of IL and enhancing the mass transfer [104]. Therefore, enzymes showed higher activity and stability in the mixture solvents of IL and SC-CO 2 than the corresponding single medium.…”

Section: Biocatalysis In Combined Mixture Solvents Of Ionic Liquid Anmentioning

confidence: 88%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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Abstract:Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water-dependent side reactions. It has been demonstrated that enzyme has high activity and stability in non-aqueous media, and the variation of enzyme activity is attributed to its conformational modifications. This review comprehensively addresses the stability and activity of the intact enzymes in various non-aqueous systems, such as organic solvents, ionic liquids, sub-/super-critical fluids and their combined mixtures. It has been revealed that critical factors such as Log P, functional groups and the molecular structures of the solvents define the microenvironment surrounding the enzyme molecule and affect enzyme tertiary and secondary structure, influencing enzyme catalytic properties. Therefore, it is of high importance for biocatalysis in non-aqueous media to elucidate the links between the microenvironment surrounding enzyme surface and its stability and activity. In fact, a better understanding of the correlation between different non-aqueous environments and enzyme structure, stability and activity can contribute to identifying the most suitable reaction medium for a given biotransformation.

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Section: Biocatalysis In Combined Mixture Solvents Of Ionic Liquid Anmentioning

confidence: 88%

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

et al. 2016

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“…Lipase has shown good behaviour as a catalyst in nonconventional media (Paiva et al, 2002;Hernández et al, 2006). Among the ILs used for lipase-catalysed reactions, those containing alkylimidazolium cations are showed to be one of the most interesting.…”

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confidence: 99%

Stability of hydrolase enzymes in ionic liquids

et al. 2009

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In this work we attempted to evaluate the stability of penicillin G acylase (PGA) from Escherichia coli in their native form and free Candida antarctica lipase B (CaLB) in ionic liquids (ILs) at low water content. The hydrolysis of penicillin G to 6-aminopenicillanic acid (6-APA), and phenyl acetic acid (PAA) catalysed by PGA and the synthesis of butyl butyrate from vinyl butyrate and 1-butanol catalysed by CaLB were chosen as activity tests. The influence of these new solvents on enzyme stability was studied by incubating the enzyme (PGA or CaLB) in ILs based on dialkylimidazolium cations associated with perfluorinated and dicyanamide anions at a given temperature. Stability studies indicate that CaLB and PGA exhibited greater stability in water-immiscible ILs than in water-miscible ILs. Specifically, native PGA shows greater stability in IL media than in organic solvents.

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“…132 Ionic liquids increased the selectivity and activity of the enzymatic membrane process. 140 The cross-flow filtration technique is commonly used to adsorb enzymes onto the HFM surfaces. The technique circulates the enzymes solution only in one direction: from the shell-side to the lumen-side or vice versa as shown in Fig.…”

Section: Further Needsmentioning

confidence: 99%

Enzymatic membrane reactors: the determining factors in two separate phase operations

Agustian¹,

Kamaruddin²,

Bhatia³

2011

J of Chemical Tech & Biotech

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A two separate phase-enzymatic membrane reactor is an attractive process since it has a large interfacial area and exchange surfaces, simultaneous reaction and separation and other benefits. Many factors influence its successful operation, and these include characteristics of the enzyme, membrane, circulating fluids and reactor operations. Although the operating conditions are the main factor, other factors must be considered before, during or after its application. At the initial stage of reactor development, the solubility of substrates and products, type of operation, membrane material and size, enzyme preparation and loading procedure, and cleanliness of the recirculated fluids should be specified. The immobilization site, reactor arrangement, dissolved or no-solvent operation, classic or emulsion operation and immobilized or suspended enzyme(s) are determined later. Some factors still need further studies. Utilization of the technology is described for use from multigram-to plant-scale capacity to process racemic and achiral compounds. The racemates were resolved primarily by kinetic resolution, but dynamic kinetic resolution has been exploited. The technology focused on hydrolytic reactions, but esterification processes were also exploited.

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A new recirculating enzymatic membrane reactor for ester synthesis in ionic liquid/supercritical carbon dioxide biphasic systems

Cited by 77 publications

References 21 publications

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

Enzyme Stability and Activity in Non-Aqueous Reaction Systems: A Mini Review

Stability of hydrolase enzymes in ionic liquids

Enzymatic membrane reactors: the determining factors in two separate phase operations

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