A New Rat Model of Reversible Global Cerebral Ischemia

Abstract

A new rat model of global cerebral ischemia-reperfusion was proposed via reversible occlusion of the major vessels originating from the aortic arch and supplying the brain. This technique can be used for the search and study of exogenous (pharmacological) and endogenous methods of brain protection from ischemia-reperfusion injury.

Evaluation of Survival and Neurological Deficit in Rats in the New Model of Global Transient Cerebral Ischemia

“…On day 1 after GTCI, 30.4% animals died in the experimental group, which roughly corresponds to survival described by the authors of the model [1]. Mortality increased to 43.5% on day 3 and to 52.2% on day 5 (Table 1).…”

“…GTCI reperfusion model was reproduced by the method of [1] in our modifi cation. The rats anesthetized with chloral hydrate (450 mg/kg intraperitoneally) were intubated without tracheal injury and then breathed spontaneously, because approach to blood vessels supplying the brain used by us excluded opening of the pleural cavity.…”

“…The new way of GTCI modeling in rats by reversible occlusion of the great arteries branching from the aortic arch and supplying the brain was proposed as an alternative method [1]. In anesthetized rats, the great vessels extending from the aortic arch were clipped via the chest approach.…”

“…At the same time, it is quite traumatic due to damage to the pleural cavity leading to pneumothorax. Mortality in a group of sham-operated animals refl ects the severity of the surgery [1].…”

“…The aim of this work was to improve GTCI model [1], namely to reduce surgical trauma, and to assess survival and neurological defi cits in the animals after GTCI simulation by the proposed method.…”