A New Rapid Quantitative Test for Fecal Calprotectin Predicts Endoscopic Activity in Ulcerative Colitis

Abstract

FC determined by QPOCT was an accurate surrogate marker of "endoscopic remission" in UC and presented a good correlation with the FC-ELISA test.

High Within-day Variability of Fecal Calprotectin Levels in Patients with Active Ulcerative Colitis

“…This FC-QPOCT testing demonstrated a good correlation with the conventional enzymelinked immunosorbent assay test for FC determination 20,21 even for Crohn's disease and UC. 2,22 Because all patients had an active disease at the time of sample collection, the high-range FC-QPOCT kit was initially used for FC measurement in all samples. All samples scoring .1800 mg/kg were performed again after sample dilution to obtain their exact value.…”

The Relation Between Fecal Calprotectin and the Rate of Clinical Activity of Ulcerative Colitis

“…In China, Jong's test was based on Nycotest Phical ELISA (Nycomed, Norway) kit (18). And in another study FC was measured by both Calpro Elisa and Buhlmann Elisa and it was concluded that Calpro Elisa is a more accurate method (19). Iglesias studies were done using Rapid test.…”

Inflammatory bowel diseases: from pathogenesis to laboratory testing

“…Three types of FC-POCT (qualitative, semi-quantitative and quantitative) are available -and good correlations between the latter and ELISAs have been reported [54,[65][66][67][68]. However, it has yet to be established whether FC-POCT results call for ELISA confirmation, and the way in which quality should be monitored.…”

“…Calprotectin, a 36.5 kDa hetero-trimer, consisting of one S100A8 light chain and two S100A9 heavy chains belonging to the S100 calcium binding proteins family, is present in the cytoplasm of granulocytes and accounts for about 60% of soluble proteins; it is secreted extracellularly from stimulated neutrophils and monocytes or released by cell disruption or death [44]. Both lactoferrin and calprotectin have antimicrobial properties: histidine-based zinc-binding sequences (His-X-X-X-His motif) are involved in the antimicrobial activity of calprotectin, while the amino-terminal domain (lactoferricin) liberated by pepsin Røseth et al [47] CD and UC 77%/100% Solem et al [48] CD 54%/75% D'Incà et al [49] CD 77%/80% 81%/80% D'Incà et al [49] UC 75%/60% 78%/70% Sipponen et al [50] CD 77%/100% 87%/100% Schoepfer et al [51] UC 86%-93%/71%-88% Schoepfer et al [52] CD 89%/58% 68%/58% Sipponen et al [53] CD 80%/67% 80%/89% Lobatòn et al [54] UC 74%/90% 84%/38%…”

“…Table 2 reports the sensitivity and specificity of lactoferrin, calprotectin and of CRP in discriminating between active and inactive IBDs [47][48][49][50][51][52][53][54]. High levels of calprotectin/lactoferrin are also predictive of relapse for both UC and CD [55,56].…”