A new rapid micromethod for the assay of phenobarbital from dried blood spots by LC‐tandem mass spectrometry

Marca, Giancarlo la; Malvagia, Sabrina; Filippi, Luca; Luceri, Francesca; Moneti, Gloriano; Guerrini, Renzo

doi:10.1111/j.1528-1167.2009.02204.x

Cited by 50 publications

(30 citation statements)

References 12 publications

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“…The acceptable LLOQ is defi ned as the lowest analyte concentration that gives accuracy within ±20% (bias) of the nominal value and precision ≤20% (CV) from six replicates for a predefi ned dynamic range. Considering the limited blood volume taken from a punch, a larger punch or multiple punches make the lower concentration of analyte measurable (Alfazil and Anderson, 2008;Garcia Boy et al, 2008;Burhenne et al, 2008;Gempel et al, 2000;Higashi et al, 2008;la Marca et al, 2008bla Marca et al, , 2009Liang et al, 2009). On the other hand, a larger punch from a small spot might result in a better reproducibility and a small punch from a large area might be one of the sources of poor assay precision due to the chromatographic eff ect (Hoogtanders et al, 2007).…”

Section: Assay Performancementioning

confidence: 99%

“…Second, it off ers a simpler storage and easier transfer because there is no requirement for freezers or dry ice in most applications. DBS can be conveniently collected by patients themselves or guardians with minimum training and sent by mail to the assigned laboratory, avoiding unnecessary costs (Garcia Boy et al, 2008;la Marca et al, 2009). Third, DBS reduces the infection risk of HIV/AIDS and other infectious pathogens to a minimum (Parker and Cubit, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

535

428

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The collection of whole blood samples on paper, known as dried blood spot (DBS), dates back to the early 1960s in newborn screening for inherited metabolic disorders. DBS off ers a number of advantages over conventional blood collection. As a less invasive sampling method, DBS off ers simpler sample collection and storage and easier transfer, with reduced infection risk of various pathogens, and requires a smaller blood volume. To date, DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecules. Despite the increasing popularity of DBS-LC-MS/MS, the method has its limitations in assay sensitivity due to the small sample size. Sample quality is often a concern. Systematic assessment on the potential impact of various blood sample properties on accurate quantifi cation of analyte of interest is necessary. Whereas most analytes may be stable on DBS, unstable compounds present another challenge for DBS as enzyme inhibitors cannot be conveniently mixed during sample collection. Improvements on the chemistry of DBS card are desirable. In addition to capturing many representative DBS-LS-MS/MS applications, this review highlights some important aspects of developing and validating a rugged DBS-LC-MS/MS method for quantitative analysis of small molecules along with DBS sample collection, processing and storage.

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Section: Assay Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

535

428

View full text Add to dashboard Cite

show abstract

“…Some studies recently evaluated the use of DBS for therapeutic drug monitoring (TDM) and toxicology in adults [9,10]. Some studies, also from our group, applied the DBS technique to evaluate drug concentrations in newborns, infants and children [11][12][13][14][15]. These reports showed that the accuracy of TDM studies using DBS is comparable to that of traditional TDM studies on plasma which require larger blood volumes.…”

Section: Introductionmentioning

confidence: 66%

Development of an UPLC–MS/MS method for the determination of antibiotic ertapenem on dried blood spots

Marca

Giocaliere

Villanelli

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tErtapenem (Invanz ® ) is a newly developed carbapenem ␤-lactam antimicrobial agent. The drug usage in pediatric age needs an accurate drug monitoring for effective patient management. The aim of this study was to evaluate the use of dried blood spot (DBS) specimens to measure ertapenem concentration during treatment. The analysis was performed by UPLC-MS/MS operating in multiple reaction monitoring (MRM) mode. The calibration curve in matrix was linear in the concentration range of 0.5-100 mg/L with correlation coefficient value higher than 0.997. Performance parameters of this method like lower limit of detection (LLOD, 0.2 mg/L), lower limit of quantification (LLOQ, 0.5 mg/L), matrix effect (20%), intra-and inter-day imprecision (CV within than 15%) and accuracy (between 94 and 155%) of drug concentrations have been evaluated. The drug stability at different temperatures was tested for one month, to evaluate the risks of sample delivery at different climatic conditions.The reported method allows now ertapenem analysis and offers many advantages for patients including the possibility of collecting samples at home.This new assay is both precise and accurate and is especially suitable for therapeutic drug monitoring and pharmacokinetic studies in neonates in whom obtaining larger blood samples is not convenient or possible.

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“…An attracting and potential method to easily obtain samples for PK studies and TDM in children is collection of whole blood samples onto filter paper (dried blood spot or DBS) [7][8][9][10][11][12]. DBS are commonly used for neonatal screening of metabolic diseases, cystic fibrosis and hypothyroidism in micro-blood samples.…”

Section: Introductionmentioning

confidence: 99%

“…DBS are commonly used for neonatal screening of metabolic diseases, cystic fibrosis and hypothyroidism in micro-blood samples. Some studies recently evaluated the use of DBS for TDM and toxicology in adults [7][8][9][10][11][12]. Some authors, also from our group, applied the DBS technique to evaluate drug concentrations in newborns, infants and children [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive LC–MS/MS method for the analysis of antibiotic linezolid on dried blood spot

Marca

Villanelli

Malvagia

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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a b s t r a c tLinezolid is a new drug from the oxazolidinone class of antibiotics used against mycobacteria and multidrug resistant (MDR) Gram-positive bacterial infections, which may are also glycopeptide-resistant. The drug usage in pediatric age needs an accurate drug monitoring for effective patient management. The aim of this study was to evaluate the use of dried blood spot (DBS) specimens to determinate linezolid levels during treatment. Advantages of DBS include short collection time, low invasiveness, ease and low cost of sample collection, transport and storage. The analysis was performed in LC-MS/MS operating in positive ion mode and multiple reaction monitoring (MRM) mode. The calibration curve in matrix was linear in the concentration range of 1-100 mg/L with correlation coefficient value of 0.9987. Intraday and interday coefficients of variation were within 3.6% and 13.0%, respectively. We also tested the thermal and temporal drug stability in dried blood spots at four different temperatures to evaluate the risks of sample delivery in different conditions. The short term stability studies showed that linezolid concentration remained stable for at least one month under all the conditions tested.This new assay has favorable characteristics being highly precise and accurate and allows a fast linezolid analysis with a total run time 22 min long, in gradient analysis. Concentration data for plasma and DBS samples from patients after treatment were compared showing a good correlation.Correlation between DBS data and serum samples measured by HPLC-UV was satisfactory. The benefit for patients is the ability to monitor the treatment with a simple and convenient sample collection at home.

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A new rapid micromethod for the assay of phenobarbital from dried blood spots by LC‐tandem mass spectrometry

Cited by 50 publications

References 12 publications

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Development of an UPLC–MS/MS method for the determination of antibiotic ertapenem on dried blood spots

Rapid and sensitive LC–MS/MS method for the analysis of antibiotic linezolid on dried blood spot

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