A new pyrrole alkaloid isolated fromArum palaestinum Boiss. and its biological activities

Abstract

The phytochemical analysis of the ethyl acetate fraction of Arum palaestinum Boiss. (Araceae) led to the isolation and identification of a new polyhydroxy alkaloid compound; (S)-3,4,5-trihydroxy-1 H-pyrrol-2(5H)-one (1), and other five known compounds; caffeic acid (2), isoorientin (3), luteolin (4) and vicenin 11 (5), as well as the rare compound 3,6,8-trimethoxy, 5,7,3',4'-tetrahydroxy flavone (6). The structural elucidations of all the compounds were based on spectroscopic data (1H- and 13C-NMR, DEPT, HSQC, HMBC and NOE difference techniques) and comparison with literature data. Investigation of the antioxidant activity of the ethyl acetate fraction indicated its strong scavenging capacity for 1,1 -diphenyl-2-picrylhydrazyl (DPPH) radicals (SC50 3.1+/-0.82 microg/mL). Moreover, the treatment of different human cancer cell lines with the ethyl acetate fraction led to dose-dependant suppression in the proliferation of both breast carcinoma cells (MCF-7; IC50 59.09+/-4.1 microg/mL) and lymphoblastic leukemia cells (1301; IC50 53.1+/-2.9 microg/mL); however, it was found to have no effect on the growth of hepatocellular carcinoma cells (Hep G2).

A new antioxidant compound fromCapparis spinosa

“…The antioxidant capacity of these compounds was carried out by determination photometrically through their scavenging activity against the stable artificial free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH•), using the method as described previously (El-Desouky et al, 2007) with slight modification. In brief, 200 μL of 0.208 mM DPPH solution (in ethanol) was added to an Eppendorf tube containing a 200 μL aliquot of different concentrations sample (in water).…”

Arum: a plant genus with great medicinal potential

“…But the isolation and characterization of these compounds from Arum started relatively late. 89 The polyhydroxy alkaloid that was isolated in the same year, 90 provides an interesting starting material for synthetic purposes. Two other new alkaloids were reported later, and they have even simpler structures.…”

“…But studying other reports show that this compound is present in A. palaestinum, and many of its derivatives. 90 …”

GC/MS Analysis of the Volatile Constituents from Arum Cyreniacum Flowers

“…Fifty-six compounds were identified from the odour of A. maculatum. The major components being: 2-heptanone, indole, p.cresol, Also, several ketones and esters were detected and the majority of the compounds were terpenoids [8]. By reviewing the available literature, it was found nothing about the chemical constituents of the volatiles from the flowers of Arum cyrenicum so the aim of this study is the isolation and identification of volatile constituents from the Arum cyrenicum flowers using solvent extraction.…”

“…The phytochemicals like volatile oil, terpenes, flavonoids, lectins and alkaloids were studied in Arum genus. Kite et al [8][9][10] studied the odours of many Arum species and they identified 36 compounds, in which butanoïc acid esters, 1-decene, terpenes (citronellene and its derivatives), p-cresol, methyl salicylate, indole and 2-heptanone are common compounds. Some species appear clearly different like A. creticum or A. palaestinum, whose rotten fruit odour is due to benzyl alcohol and ethyl acetate.…”