A new potentiometric sensor for the determination of α-amylase activity

Sakač, Nikola; Sak-Bosnar, Milan; Horvat, Marija; Madunić-Čačić, Dubravka; Széchenyi, Aleksandar; Kovács, Barna

doi:10.1016/j.talanta.2010.11.053

Cited by 27 publications

(12 citation statements)

References 26 publications

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“…These assays are mainly spectrophotometric, and require tedious sample preparation and are often high in cost. Sakač et al 52 present a theoretical approach to measuring α-amylase using a potentiometric sensor. The sensor design employed a platinum redox electrode and would be able to measure triiodide released from α-amylase promoted starch degradation.…”

Section: Potentiometric Sensorsmentioning

confidence: 99%

Electrochemical Sensors and Biosensors

et al. 2011

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By using a solid KCl melt in conjunction with an Ag/AgCl component, Vanau et al. 8 were able to fabricate an SSRE designed specifically for beverage industry applications. They found that this SSRE had a wide range of pH use, stable potentials, and small drift potentials (1 mV at room temperature over 3 months time). The reported stability and lifetime of this reference electrode make it particularly applicable for the food industry. In addition to food industry, engine diagnostics are utilizing SSRE to assess efficiency. Oxygen sensors have long been utilizing yttria-stabilized zirconia-based potentiometric components. Recently, Mn-based oxides have been studied to find a suitable SSRE to ensure accurate on-board diagnosis for engines. Miura et al. 9 found Mn 2 O 3 -sensing electrodes to function with excellent sensitivity and has great potential for miniaturization.Rius-Ruiz et al. 10 recently reported a carbon nanotube (CNT)-based SSRE. The most successful design tested utilized a photopolymerized n-butyl acrylate polymer in conjuction with SWCNTs, acting as the transducer layer. This type of transducer layer was often superior to alternative solid transducers, and the resulting SSRE proved insensitive to room lighting. The superior characteristics of this reference electrode as well as its ease of fabrication allow for potential widespread usage in a multitude of systems.Other Papers of Interest. To ensure reference electrodes keep up with current miniaturization trends, it has become challenging to find suitable materials for all potential applications. One such challenging arena is that of steel corrosion monitoring. Muralidharan et al. 11 developed a NiFe 2 O 4 reference electrode due to its superior fabrication cost and ease and its resistance to corrosion. The resulting electrode exhibited stability and low polarization currents in a calcium hydroxide solution, which is commonly used in concrete environments.Shibata et al. 12 examined the stability of an Ag/AgCl reference electrode with the novel salt bridge 1-methyl-3-octylimidazolium bis(trifluoromethanesulfonyl)-amide. This bridge was proposed and found to be superior in order to avoid the interference found using alternate ionic liquid salt bridges, such as KCl, while experimenting in phthalate buffer. Further evaluation of stability is necessary; however, the initial findings suggest this ionic liquid salt bridge is promising. Huang et al. 13 recently developed a working system using a Nafion strip membrane as an ion-conducting bridge, allowing for electrochemical measurements in pure water. By using this bridge, it is possible to avoid potential leaching contaminants and gain higher accuracy.Measuring the electrodeposition of boron melts is a challenge to researchers due to the unknown chemistry of KF-KCl-KBrF 4 . Pal et al. 14 fabricated an Ag/AgCl reference electrode that was found to be reversible and to have suitable stability and nonpolarizability. This will allow for the first available measurements of KF-KCl-KBrF 4 chemistry at high tempera...

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Section: Potentiometric Sensorsmentioning

confidence: 99%

Electrochemical Sensors and Biosensors

et al. 2011

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“…Since the majority of the reported methods are cumbersome, less reliable, and take a lot of time to perform, most of these papers are based on modifying the available methods to increase accuracy and ease of use. In the literature, several papers describing different kinds of analytical techniques to determine α ‐amylase activity can be found . For example, techniques such as spectrometry, fluorometry, amperometry, electrophoresis, isoelectric focusing, chromatography, and immunological methods have been adapted to detect α ‐amylase activity.…”

Section: Introductionmentioning

confidence: 99%

“…In the literature, several papers describing different kinds of analytical techniques to determine α ‐amylase activity can be found . For example, techniques such as spectrometry, fluorometry, amperometry, electrophoresis, isoelectric focusing, chromatography, and immunological methods have been adapted to detect α ‐amylase activity. In addition, several methods have emerged that use different substrates, including starch, amylose, amylopectin, and some chemically modified derivatives of polymers and maltooligosaccharides of varying chain length linked to a chromophore, such as 4‐nitrophenyl or 2‐chloro‐4‐nitrophenyl .…”

Section: Introductionmentioning

confidence: 99%

Critical review on conventional spectroscopic α‐amylase activity detection methods: merits, demerits, and future prospects

et al. 2020

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α‐Amylase is an endoenzyme that catalyses the hydrolysis of internal α‐l,4 glycosidic linkages in polysaccharides to produce maltose, maltotriose, and α‐limit dextrins. It is widely used in the laboratorial and industrial workflow for several applications. There are several methods utilizing different techniques and substrates to assess α‐amylase activity, among which the spectroscopic methods have found widespread applicability due to their ease of use and cost‐effectiveness. Depending upon the reaction principle, these assays are classified into four groups: reducing sugar, enzymatic, chromogenic, and amyloclastic methods. Despite the presence of numerous methods, there is no general reliable method to assess α‐amylase activity. Each method is shown to have its own merits and demerits. Many improvements have been made to make the available methods more accurate, reliable, and easy. This communication briefly discusses the basic reaction mechanisms and critically reviews the advantages and shortcomings associated with each method. Further recommendations are made for future development. © 2020 Society of Chemical Industry

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“…Recently, several interesting high-throughput screening approaches have been presented, like droplet-based microfluidic system for rapid encapsulation, cultivation, assaying and sorting of yeast cells expressing heterologous hydrolytic enzymes (Beneyton et al 2017), protocols for high-throughput cultivation screening with real-time data acquisition (Back et al 2016), fluorescence-activated cell sorting (FACS) of cell-surface displayed protein mutants (Olsen et al 2000; Mattanovich and Borth 2006), or miniaturized potentiometric sensors (Sakač et al 2011) or immunosensors (Della Ventura et al 2017) for the determination of enzymatic activity. These techniques exhibit superiority in terms of high-throughput character, time-efficiency, accuracy, and precision; however, they require specialized equipment or specific structure of the DNA constructs, which might be considered as a kind of limitation.…”

Section: Introductionmentioning

confidence: 99%

Rapid micro-assays for amylolytic activities determination: customization and validation of the tests

Borkowska

Białas

Kubiak

et al. 2019

Appl Microbiol Biotechnol

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High-throughput function-based screening techniques remain the major bottleneck in the novel biocatalysts development pipeline. In the present study, we customized protocols for amylolytic activity determination (Somogyi-Nelson and starch-iodine tests) to micro-volume thermalcycler-based assays (linearity range 60–600 μM of reducing sugar, R2 = 0.9855; 0–2 mg/mL of starch, R2 = 0.9921, respectively). Exploitation of a thermalcycler enabled rapid and accurate temperature control, further reduction of reagents and samples volumes, and limited evaporation of the reaction mixtures, meeting several crucial requirements of an adequate enzymatic assay. In the optimized micro-volume Somogyi-Nelson protocol, we were able to reduce the time required for high-temperature heating sixfold (down to 5 min) and further increase sensitivity of the assay (tenfold), when compared to the previous MTP-based protocol. The optimized microassays have complementary scope of specificities: micro-starch-iodine test for endoglucanases, micro-Somogyi-Nelson test for exoglucanases. Due to rapid, micro-volume and high-throughput character, the methods can complement toolbox assisting development of novel biocatalysts and analysis of saccharides-containing samples.Electronic supplementary materialThe online version of this article (10.1007/s00253-018-09610-0) contains supplementary material, which is available to authorized users.

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A new potentiometric sensor for the determination of α-amylase activity

Cited by 27 publications

References 26 publications

Electrochemical Sensors and Biosensors

Electrochemical Sensors and Biosensors

Critical review on conventional spectroscopic α‐amylase activity detection methods: merits, demerits, and future prospects

Rapid micro-assays for amylolytic activities determination: customization and validation of the tests

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