A New Plant Expression System for Producing Pharmaceutical Proteins

Abd-Aziz, Nazrin; Tan, Boon Chin; Rejab, Nur Ardiyana; Othman, Rofina Yasmin; Khalid, Norzulaani

doi:10.1007/s12033-020-00242-2

Cited by 13 publications

(9 citation statements)

References 73 publications

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“…Third, although in this study we only used N. benthamiana , we anticipate that the agromonas assay can be easily adapted to plant species suitable for agroinfiltration, such as potato (Du et al ., 2014), tobacco (Van der Hoorn et al ., 2000), pea (Guy et al ., 2016), Medicago (Picard et al ., 2013), grapefruit (Figueiredo et al ., 2011), lettuce (Chen et al ., 2016), tomato (Wroblewski et al ., 2009), flax (Dodds et al ., 2006), cassava (Zeng et al ., 2019), strawberry (Guidarelli and Baraldi, 2015) and Mucuna bracteate (Abd‐Aziz et al ., 2020). This facilitates studies of immunity in other plant species using corresponding P. syringae pathovars.…”

Section: Discussionmentioning

confidence: 99%

Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves

et al. 2021

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Section: Discussionmentioning

confidence: 99%

Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves

et al. 2021

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“…Other options include using cost-effective expression systems for high yields of folded NIPs — e.g. transient [ 84 , 85 ] or stable [ 86 ] plant systems allow for folding and multimeric protein assembly. With the latter systems, the yields and quality of expressed NIPs could be better than those stemming from the traditionally used microbial hosts.…”

Section: Progress In Biotechnologies Enhances Definitions Of Transpormentioning

confidence: 99%

Plant transporters involved in combating boron toxicity: beyond 3D structures

Hrmová

Gilliham

Tyerman

2020

Biochemical Society Transactions

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Membrane transporters control the movement and distribution of solutes, including the disposal or compartmentation of toxic substances that accumulate in plants under adverse environmental conditions. In this minireview, in the light of the approaching 100th anniversary of unveiling the significance of boron to plants (K. Warington, 1923; Ann. Bot.37, 629) we discuss the current state of the knowledge on boron transport systems that plants utilise to combat boron toxicity. These transport proteins include: (i) nodulin-26-like intrinsic protein-types of aquaporins, and (ii) anionic efflux (borate) solute carriers. We describe the recent progress made on the structure–function relationships of these transport proteins and point out that this progress is integral to quantitative considerations of the transporter's roles in tissue boron homeostasis. Newly acquired knowledge at the molecular level has informed on the transport mechanics and conformational states of boron transport systems that can explain their impact on cell biology and whole plant physiology. We expect that this information will form the basis for engineering transporters with optimised features to alleviate boron toxicity tolerance in plants exposed to suboptimal soil conditions for sustained food production.

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“…For instance, the organized head structure of Lactuca sativa (lettuce) allows for more effective transient transformation than the unorganized leaves of Nicotiana tabacum (tobacco) [24] . Also, Mucuna bracteata leaves allow for a higher level of transient expression of recombinant proteins than Nicotiana benthamiana leaves [25] . However, the advantage of Nicotiana spp.…”

Section: Introductionmentioning

confidence: 99%

Transient recombinant expression of highly immunogenic CagA, VacA and NapA in Nicotiana benthamiana

Barzigar¹,

Haraprasad

Kumar³

et al. 2022

Biotechnology Reports

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Highlights A platform for rapid expression of genes through agro-infiltration is a suitable alternative to the laborious process of generating stable transgenic lines for producing recombinant immunogenic antigens in plants. This study has successfully developed and demonstrated a reproducible transformation protocol for rapid expression of Helicobacter pylori recombinant CagA, VacA and NapA antigens in Nicotiana benthamiana . This is the first study of recombinant expression CagA, VacA and NapA gene of Helicobacter pylori in Nicotiana benthamiana via syringe-assisted A grobacterium infiltration. A syringe infiltration of a four to five weeks old Nicotiana benthamiana plant with Agrobacterium tumefaciens subtype EHA105 was optimal to produce the maximum mRNA levels of CagA, VacA and NapA mRNA in leaf at the third-day post-Agro-infiltration.

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A New Plant Expression System for Producing Pharmaceutical Proteins

Cited by 13 publications

References 73 publications

Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves

Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves

Plant transporters involved in combating boron toxicity: beyond 3D structures

Transient recombinant expression of highly immunogenic CagA, VacA and NapA in Nicotiana benthamiana

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