A new pilot-scale fermentation mode enhances Euglena gracilis biomass and paramylon (β-1,3-glucan) production

Wu, Mingcan; Qin, Huan; Deng, Jiuqi; Liu, Yongqi; Lei, Anping; Zhu, Hongxiang; Hu, Zhangli; Wang, Jiangxin

doi:10.1016/j.jclepro.2021.128996

Cited by 32 publications

(20 citation statements)

References 40 publications

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“…The Euglena gracilis CCAP 1224/5Z powders (autotrophic Euglena as “AE,” heterotrophic Euglena as “HE” afterward) were supplied by Yougetiancheng Biotechnology Co., (Yiwu, China) ( 15 ). The nutrients in the Euglena powders, such as paramylon, total proteins, lipids, amino acids, vitamins, and minerals, were determined using standard methods.…”

Section: Methodsmentioning

confidence: 99%

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Dai

He²,

Chen³

et al. 2022

Front. Nutr.

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Euglena gracilis, a single-celled microalga with various trophic growth styles under different cultivation conditions, contains nutrients, such as ß-1,3-glucans, essential amino acids, fatty acids, vitamins, and minerals. It has recently attracted attention as a new health food. Among them, ß-1,3-glucans, paramylon of Euglena, is an insoluble dietary fiber and is well known as an immune booster, attenuator of obesity and diabetes, reducer of acute liver injury, and suppressor of atopic dermatitis, and other chronic inflammatory disorders. Recently, evidence has appeared for the positive health effects of foods, food ingredients, or biochemical compounds derived from several other microalgae, such as Chlorella, Spirulina, Dunaliella, Phaeodactylum, and Pavlova. Until most recently, the prebiotic activity of Euglena and paramylon was reported. Emerging prospects of microalgae as prebiotics were well summarized, but the mechanisms behind the bacterial growth promotion by microalgae are not elucidated yet. Thus, we evaluated the prebiotic prospects of both autotrophic and heterotrophic Euglena on six different Lactobacillus. What’s more, the stimulated mechanism was revealed by bacterial culture medium metabolomic analysis. This study could widen the knowledge about the prebiotic activity of Euglena as a next-generation prebiotic and other microalgae-derived compounds as potential health foods.

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Section: Methodsmentioning

confidence: 99%

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Dai

He²,

Chen³

et al. 2022

Front. Nutr.

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“…For in situ microbial population analysis, the top 10 with the most abundance were analyzed at the phylum level and relative abundance histograms and cluster heatmaps were created. For laboratory microbial population analysis, the sample data were analyzed using the principal component analysis (PCA) ( Wu et al, 2021 ). The microbial communities within the individual sample and group were analyzed at the phylum level.…”

Section: Methodsmentioning

confidence: 99%

Euendolithic Cyanobacteria and Proteobacteria Together Contribute to Trigger Bioerosion in Aquatic Environments

Huang²,

Wang³

et al. 2022

Front. Microbiol.

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Shellfish, mussels, snails, and other aquatic animals, which assimilate limestone (calcium carbonate, CaCO3) to build shells and skeletons, are effective carbon sinks that help mitigate the greenhouse effect. However, bioerosion, the dissolution of calcium carbonate and the release of carbon dioxide, hinders carbon sequestration process. The bioerosion of aquatic environments remains to be elucidated. In this study, the bioerosion of Bellamya spp. shells from the aquatic environment was taken as the research object. In situ microbial community structure analysis of the bioerosion shell from different geographical locations, laboratory-level infected culture, and validated experiments were conducted by coupling traditional observation and 16S rRNA sequencing analysis method. Results showed that bioeroders can implant into the CaCO3 layer of the snail shell, resulting in the formation of many small holes in the shell, which reduced the shell’s density and made the shell fragile. Results also showed that bioeroders were distributed in two major phyla, namely, Cyanobacteria and Proteobacteria. Cluster analysis showed that Cyanobacteria sp. and two unidentified genera (Burkholderiaceae and Raistonia) were the key bioeroders. Moreover, results suggested that the interaction of Cyanobacteria and other bacteria promoted the biological function of “shell bioerosion.” This study identified the causes of “shell bioerosion” in aquatic environments and provided some theoretical basis for preventing and controlling it in the aquatic industry. Results also provided new insights of cyanobacterial bioerosion of shells and microalgae carbon sequestration.

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“…Paramecium was cultivated in hay infusion [ 70 , 71 , 72 ]. Euglena was cultivated in solution according to Wu [ 73 ]. Pentatrichomonas hominis was isolated from stool samples and kept in Pahm solution according to Chomicz et al [ 74 ]…”

Section: Methodsmentioning

confidence: 99%

“…Paramecium was cultivated in hay infusion [70][71][72]. Euglena was cultivated in solution according to Wu [73]. Pentatrichomonas hominis was isolated from stool samples and kept in Pahm solution according to Chomicz et al [74] The gregarines were isolated from cockroaches and treated with the mixtures at different concentrations after being placed on a watch glass in Ringer's solution.…”

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confidence: 99%

Novel Formula of Antiprotozoal Mixtures

et al. 2022

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Antimicrobial resistance (AMR) is becoming more common in both bacteria and pathogenic protozoa. Therefore, new solutions are being sought as alternatives to currently used agents. There are many new ideas and solutions, especially compounds of natural origin, including essential oils. In the present study, the antiprotozoal activity of a mixture of essential oils (eucalyptus, lavender, cedar and tea tree), organic acids (acetic acid, propionic acid and lactic acid) and metal ions (Cu, Zn, Mn) were tested. As a model, protozoans were selected: Euglena gracilis, Gregarina blattarum, Amoeba proteus, Paramecium caudatum, Pentatrichomonas hominis. The tested concentrations of mixtures were in the range of 0.001–1.5%. The analyses show unexpected, very strong protozoicidal activity of combinations, presenting the synergy of compounds via determination of LD50 and LD100 values. Obtained mixtures showed significantly higher activity against protozoans, compared to chloramphenicol and metronidazole. Most of the analyzed samples show high antiprotozoal activity at very low concentration, in the range of 0.001–0.009%. The most effective combinations for all analyzed protozoans were the cedar essential oil and tea tree essential oil with a mixture of acids and manganese or zinc ions. Innovative combinations of essential oils, organic acids and metal ions are characterized by very high antiprotozoal activity at low doses, which, after further investigation, can be applicable for control of protozoan pathogens.

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A new pilot-scale fermentation mode enhances Euglena gracilis biomass and paramylon (β-1,3-glucan) production

Cited by 32 publications

References 40 publications

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Euglena gracilis Promotes Lactobacillus Growth and Antioxidants Accumulation as a Potential Next-Generation Prebiotic

Euendolithic Cyanobacteria and Proteobacteria Together Contribute to Trigger Bioerosion in Aquatic Environments

Novel Formula of Antiprotozoal Mixtures

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