A New Phenotypic Lexicon for Accelerated Translation

MacRae, Calum A.

doi:10.1161/circulationaha.114.014067

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…The subset of disease models for which a strong disease-specific phenotype is uncovered in the Cell Painting assay are then systematically screened against a drug-repurposing library to identify drugs that can reduce the strength of the disease phenotype and thus rescue the putative disease-specific features of the profile. Ultimately, the goal is to find new indications for existing drugs; this general approach (using an assay of three stains rather than the Cell Painting assay) has already been used successfully to identify potential new uses of known drugs for the treatment of cerebral cavernous malformation, a hereditary stroke syndrome 32,33 .…”

Section: Introductionmentioning

confidence: 99%

Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes

et al. 2016

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In morphological profiling, quantitative data are extracted from microscopy images of cells to identify biologically relevant similarities and differences among samples based on these profiles. This protocol describes the design and execution of experiments using Cell Painting, a morphological profiling assay multiplexing six fluorescent dyes imaged in five channels, to reveal eight broadly relevant cellular components or organelles. Cells are plated in multi-well plates, perturbed with the treatments to be tested, stained, fixed, and imaged on a high-throughput microscope. Then, automated image analysis software identifies individual cells and measures ~1,500 morphological features (various measures of size, shape, texture, intensity, etc.) to produce a rich profile suitable for detecting subtle phenotypes. Profiles of cell populations treated with different experimental perturbations can be compared to suit many goals, such as identifying the phenotypic impact of chemical or genetic perturbations, grouping compounds and/or genes into functional pathways, and identifying signatures of disease. Cell culture and image acquisition takes two weeks; feature extraction and data analysis take an additional 1-2 weeks.

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Section: Introductionmentioning

confidence: 99%

Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes

et al. 2016

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show abstract

“…We then screen a drug-repurposing library to identify drugs that can reduce the strength of the disease phenotype and thus rescue the putative disease-specific features of the profile. Ultimately, the goal is to find new indications for existing drugs; this general approach (using an assay of three stains rather than the Cell Painting assay) has already been used successfully to identify potential new uses of known drugs for the treatment of cerebral cavernous malformation, a hereditary stroke syndrome 31,32 .…”

Section: Applications Of the Methodsmentioning

confidence: 99%