A new pharmacological testing method. Different effects of levamisole and the serum of mice orally treated with levamisole on mitogenic activity of lipopolysaccharide.

Abstract

The direct addition of levamisole to murine splenic lymphocytes had no effect on the mitogenic activity of lipopolysaccharide. However, the addition of serum of mice orally treated with levamisole increased the mitogenic activity, and this increased activity using serum was similar to the result obtained in an in vivo experiment. These results suggest that the new in vitro experimental method using serum may be able to reproduce the in vivo effect of drugs.

Bushen Ningxin Decoction pharmacological serum promotes the proliferation and suppresses the apoptosis of murine osteoblasts through MAPK pathway

“…Henceforth, it was also confirmed that the direct addition of levamisole to murine splenic lymphocytes had no effect on the mitogenic activity of LPS. However, the addition of serum from mice orally treated with levamisole increased the mitogenic activity, and this increased activity using serum was similar to the result obtained in an in vivo experiment (Amagaya et al, 1989). These suggest that the new in vitro experimental method using serum may be able to reproduce the in vivo effect of drugs and the new pharmacological testing method pro- Fig.…”

Liuwei Dihuang decoction facilitates the induction of long-term potentiation (LTP) in senescence accelerated mouse/prone 8 (SAMP8) hippocampal slices by inhibiting voltage-dependent calcium channels (VDCCs) and promoting N-methyl-d-aspartate receptor (NMDA) receptors

“…The disruption of gene expression can be corrected by LW in the hippocampal formation of SAMP8 (Cheng et al, 2007). Serum pharmacology has proven to be an effective method to study TCM prescriptions (Amagaya et al, 1989;Iwama et al, 1987;Zhang et al, 2000). According to the concept of serum pharmacology, serum from animals administered a TCM prescription orally is widely used to uncover the mechanisms of TCM prescriptions that contain too many unidentified compounds.…”

Effects of Liuwei Dihuang decoction on ion channels and synaptic transmission in cultured hippocampal neuron of rat

“…In recent years, serum pharmacology was put forward and proved to be effective in pharmacological research on TCM prescription (Iwama et al, 1987;Amagaya et al, 1989;Zhang et al, 2000). According to the concept of serum pharmacology, serum from animals administered with TCM prescription orally is widely used to uncover the mechanism of TCM prescription that content too many unidentified compounds.…”